BD, 559438, BD Pharmingen™ Purified NA/LE Rat Anti-Mouse CD1d

Alternative Name: Cd1d1; Cd1.1; Cd1a; Cd1d; Ly-38
Reactivity: Mouse (QC Testing)
Isotype: Rat LEW, also known as Lewis IgG2b, κ
Immunogen: Mouse Cd1.1 cDNA-transfected RMA-S mouse T lymphoma and L929 cells
Application: Flow cytometry (Routinely Tested), Blocking, Immunoprecipitation (Reported)
Concentration: 1.0 mg/ml
RRID: AB_397242
Storage Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
Recommended Assay Procedures: Recommended Assay Procedures Reported applications include immunoprecipitation, blocking of in vitro antigen-mediated stimulation of NK1+T cells, blocking of in vitro responses of some T cells to CD1d1, (possibly to the β2m-associated form of CD1d, but not to β2m-independent CD1d4), and in vivo inhibition of TGF-β2 production. mAb 3C11 (Cat. no. 559871) has been reported to inhibit responses of some T cells to both β2m-associated and β2m-independent forms of CD1d.