BD, 560818, BD Phosflow™ PE Mouse anti-IκBα

Alternative Name: MAD-3; I-kappa-B-alpha; IkB-alpha; NFKBIA; NF-kappa-B inhibitor alpha
Reactivity: Human (QC Testing)
Isotype: Mouse IgG1
Immunogen: Human IκBα aa. 145-302 Recombinant Protein
Application: Intracellular staining (flow cytometry) (Routinely Tested)
Vol. Per Test: 5 µl
RRID: AB_2033975
Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
Recommended Assay Procedures: Recommended Assay Procedures This PE-conjugated antibody is suitable for intracellular staining of cell lines and primary cells using BD Cytofix™ Fixation Buffer or BD Phosflow™ Lyse/Fix Buffer. Although this antibody can be used with BD Phosflow™ Perm/Wash Buffer I or BD Phosflow™ Perm Buffers II, III or IV, it performs optimally when used with BD Phosflow™ Perm Buffer II.