BD, 561734, BD Pharmingen™ PE Rat Anti-Mouse CD184

Alternative Name: CXCR4, C-X-C chemokine receptor type 4; Fusin; LESTR; PB-CKR; Sdf1r
Reactivity: Mouse (QC Testing)
Isotype: Rat IgG2b, κ
Immunogen: GST-NCXCR4 fusion protein
Application: Flow cytometry (Routinely Tested)
Concentration: 0.2 mg/ml
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
Recommended Assay Procedures: Recommended Assay Procedures Clone 2B11/CXCR4 has been reported to perform optimally when allowed to stain for 45 minutes.  In addition, investigators are highly encouraged to titrate the antibody up to saturating levels for optimal performance, minimizing the risk for dim staining.  Please refer to http://www.bdbiosciences.com/support/resources/ for additional resources and protocols.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors. An isotype control should be used at the same concentration as the antibody of interest.