BD, 563828, BD Pharmingen™ Q-VD-OPh, General Caspase Inhibitor

Application: Flow cytometry (Routinely Tested)
RRID: AB_2869523
Storage Buffer: Lyophilized in dimethyl sulfoxide (DMSO).
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Avoid multiple freeze-thaws of product. Store the lyophilized Q-VD-OPh inhibitor at –20°C. Reconstitute the Q-VD-OPh inhibitor in DMSO before use. The reconstituted Q-VD-OPh inhibitor may be stored in small aliquots at –20°C.
Recommended Assay Procedures: Recommended Assay Procedures The Q-VD-OPh inhibitor is designed to be used in both in vivo and in vitro cell based assays to measure the inhibition of apoptosis. Reconstitute 1.0 mg of the Q-VD-OPh inhibitor in DMSO. A 10 mM stock solution may be made by dissolving 1.0 mg of Q-VD-OPh in 195 µl DMSO. The final concentration of inhibitor may vary between experimental systems and investigators are encouraged to titrate the inhibitor for optimal performance. As a precautionary note, do not exceed a final DMSO concentration of 0.2% as higher levels may cause cellular toxicity and mask the effects of the caspase inhibitor.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.