BD, 567110, BD Pharmingen™ Purified NA/LE Hamster Anti-Mouse CD28

Alternative Name: Cd28; CD28 antigen; T-cell-specific surface glycoprotein CD28
Reactivity: Mouse (QC Testing)
Isotype: Syrian Hamster IgG2, λ1
Immunogen: Mouse EL-4 (T-cell lymphoma) Cells
Application: Flow cytometry (Routinely Tested), Immunohistochemistry-frozen (Tested During Development), (Co)-stimulation (Reported)
Concentration: 1.0 mg/ml
RRID: AB_2916447
Storage Buffer: No azide/low endotoxin: Aqueous buffered solution containing protein stabilizer, no preservative, sterile filtered(0.2µm pore size membrane). Endotoxin level is ≤0.1 EU/µg (≤0.01 ng/µg) of protein as determined by the LAL assay.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
Recommended Assay Procedures: Recommended Assay Procedures Flow Cytometry: For multicolor staining of cell suspensions from peripheral lymphoid tissues (e.g detection of T lymphocytes and/or NK cells), investigators are encouraged to utilize Purified Rat Anti-Mouse CD16/CD32 [Mouse BD Fc Block™] (Cat. No. 553141/553142) to minimize non-specific binding.  In addition, since CD28 may be expressed at low density on resting peripheral T lymphocytes, investigators may wish to amplify the staining signal by using a biotinylated second-step antibody followed by a "bright" third-step reagent, such as PE Streptavidin (Cat. No. 554061).  If Mouse BD Fc Block™ is used, investigators need to be aware of and account for any potential crossreactivity that may occur between the second-step or third-step reagents chosen with the Mouse BD Fc Block™.  Biotin Mouse Anti-Syrian Hamster IgG2 (Cat. No. 554029) would be a suggested second-step antibody for investigators to consider utilizing.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. An isotype control should be used at the same concentration as the antibody of interest. Please refer to http://regdocs.bd.com to access safety data sheets (SDS). Although hamster immunoglobulin isotypes have not been well defined, BD Biosciences Pharmingen has grouped Armenian and Syrian hamster IgG monoclonal antibodies according to their reactivity with a panel of mouse anti-hamster IgG mAbs. A table of the hamster IgG groups, Reactivity of Mouse Anti-Hamster Ig mAbs, may be viewed at http://www.bdbiosciences.com/documents/hamster_chart_11x17.pdf. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.