Biolegend, 100201, Purified anti-mouse CD3 Antibody, 50μg

CD3, also known as T3, is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 is composed of CD3ε, δ, γ and ζ chains. It forms a TCR complex by associating with TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex
50μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: γδTCR-positive T-T hybridoma D1
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested IHC-F - Verified IP, ICC - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. For immunohistochemistry on frozen tissue sections, a concentration of 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported application (for relevant formats) include: spatial biology (IBEX)1,2.
Application References(PubMed link indicates BioLegend citation): Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations: Pietras E, et al. 2016. Nat Cell Biol. 10.1038/ncb3346. PubMed Abdel-Gadir A, et al. 2019. Nat Med. 25:1164. PubMed Hamada T, et al. 2008. J Immunol. 180:1843. PubMed Robles-Valero J, et al. 2022. Mol Oncol. 16:3533. PubMed Mammadli M, et al. 2023. Int J Mol Sci. 24:. PubMed Kang YA, et al. 2023. J Exp Med. 220:. PubMed Garo LP, et al. 2021. Nat Commun. 12:2419. PubMed Bernstein DL, et al. 2020. Brain Behav Immun. 87:543. PubMed Liu CJ, et al. 2020. Neuro Oncol. 22:1276. PubMed Doll JR, et al. 2020. PLoS Pathog. 16:e1008296. PubMed Li TT, et al. 2021. BMC Immunol. 22:55. PubMed Lu X, et al. 2021. J Exp Med. 218: . PubMed Cautivo KM, et al. 2022. Immunity. 55:254. PubMed Valet C, et al. 2022. J Clin Invest. 132: . PubMed Campisi L, et al. 2022. Nature. 606:945. PubMed Halin C, et al. 2022. EMBO J. 41:e111528. PubMed Moore AR, et al. 2022. Cell Rep. 41:111651. PubMed Zhao J, et al. 2022. J Clin Invest. 132: . PubMed Glaubitz J, et al. 2023. Gut. :. PubMed Fueyo-González F, et al. 2022. Immunity. 55:459. PubMed Potempa M, et al. 2022. J Immunol. 208:1742. PubMed Gern BH, et al. 2021. Cell Host Microbe. 29(4):594-606.e6. PubMed Kulkarni NN, et al. 2020. J Invest Dermatol. 140:645. PubMed Boyd DF, et al. 2020. Nature. 587:466. PubMed Xiao J, et al. 2012. Arterioscler Thromb Vasc Biol. 32:386. PubMed Bergstrom K, et al. 2008. Infect Immun. 76:796. PubMed Mazor R, et al. 2018. Proc Natl Acad Sci U S A. 115:E733. PubMed Burrello C, et al. 2018. Front Med (Lausanne). 5:21. PubMed Hawse WF, et al. 2019. J Immunol. 203:2771. PubMed Zhao J, et al. 2021. JCI Insight. 6:. PubMed Zhang Z, et al. 2020. J Immunol. 204:3400. PubMed Xu L, et al. 2017. Int J Biol Macromol. . 10.1016/j.ijbiomac.2017.07.090. PubMed Kim CJ, et al. 2018. Immunity. 49:1034. PubMed Wang W, et al. 2018. Cancer Cell. 34:757. PubMed Pack AD, et al. 2021. Cell Reports. 36:109586. PubMed Plumlee CR, et al. 2020. Cell Host Microbe. 29(1):68-82.e5. PubMed Rasoulouniriana D, et al. 2019. J Clin Invest. 129:4151. PubMed Thulasingam S, et al. 2019. Int J Mol Sci. 20. PubMed Haque M, et al. 2021. Viruses. 13:. PubMed Wabitsch S, et al. 2021. STAR Protoc. 2:100517. PubMed Schafflick D, et al. 2020. Nat Commun. 247:11. PubMed Kerdidani D, et al. 2019. Nat Commun. 10:1405. PubMed Hayashi Y, et al. 2017. J Cancer. 8:2478. PubMed Düsedau HP, et al. 2019. Glia. 67:193. PubMed Liu T, et al. 2020. Sci Adv. 6:eaay1497. PubMed Go DM, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:715. PubMed Boothby IC, et al. 2021. Nature. 599:667. PubMed Glaubitz J, et al. 2022. Nat Commun. 13:4502. PubMed Chen Y, et al. 2018. Oncoimmunology. 7:e1388484. PubMed Child KM et al. 2018. The Journal of Neuroscience. 38(31):6806-6824 . PubMed Recino A, et al. 2019. Gene Ther. 26:40. PubMed Mayer KA, et al. 2021. FASEB J. 35:e21217. PubMed Jiang P, et al. 2021. Nat Methods. 18:1181. PubMed Zhang Z, et al. 2021. Oncoimmunology. 10:1912472. PubMed Adamson SE, et al. 2018. Arterioscler Thromb Vasc Biol. 38:1020. PubMed Shimokawa C et al. 2017. Immunity. 46(5):863-874 . PubMed Yoshihara T, et al. 2020. Gut Microbes. 11:1385. PubMed Yan J, et al. 2020. Cancer Immunol Res. 8:356. PubMed Chen L, et al. 2020. Front Immunol. 11:584458. PubMed Karhadkar TR, et al. 2021. PLoS One. 16:e0245924. PubMed Fatkhullina AR et al. 2018. Immunity. 49(5):943-957 . PubMed He LD, et al. 2021. EMBO Rep. 22:e52196. PubMed Forsyth KS, et al. 2020. PLoS Pathog. 16:e1008685. PubMed Srey MT, et al. 2020. PLoS Negl Trop Dis. 14:e0008842. PubMed Kopec A, et al. 2014. Toxicol Sci. 141:515. PubMed Mainini F, et al. 2018. Nucleic Acid Ther. 28:225. PubMed Mao FY, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:395. PubMed Zareie P, et al. 2017. J Neuroinflammation. 0.630555556. PubMed Wei S, et al. 2019. Virulence. 0.588888889. PubMed Miret JJ, et al. 2019. J Immunother Cancer. 7:32. PubMed Bittner-Eddy PD, et al. 2020. Front Immunol. 11:677. PubMed Dooley K, et al. 2021. Mol Ther. 29:1729. PubMed Liu M, et al. 2018. Diabetologia. 61:2333. PubMed Davies CL, et al. 2019. Front Immunol. 10:1048. PubMed Shin J, et al. 2018. Diabetes. 67:1068. PubMed Schuler CF, et al. 2020. Allergy. 75:2279. PubMed Lim CS, et al. 2020. Front Immunol. 11:1186. PubMed You X, et al. 2022. Front Genet. 12:790990. PubMed Karhadkar TR, et al. 2020. bioRxiv. . PubMed Clark K, et al. 2019. Front Immunol. 10:2355. PubMed Gilfillan CB, et al. 2020. Eur J Immunol. 50:505. PubMed Fitzpatrick E, et al. 2017. PLoS One. 10.1371/journal.pone.0169273. PubMed Karhadkar TR, et al. 2020. Am J Physiol Lung Cell Mol Physiol. 318:L165. PubMed Sugita S, et al. 2017. Arthritis Res Ther. 19:176. PubMed Patterson AR, et al. 2019. J Allergy Clin Immunol. 143:245. PubMed Williams SK, et al. 2018. Sci Rep. 8:13628. PubMed Bartolacci C, et al. 2018. Cancer Immunol Res. 1.281944444. PubMed Zelco A, et al. 2020. Front Cell Neurosci. 0.75625. PubMed Morgado P, et al. 2011. Infect Immun. 79:4401. PubMed Chen X, et al. 2019. Methods Mol Biol. 2048:131. PubMed Kawabe T, et al. 2017. Sci Immunol. 2:eaam9315. PubMed Moon H, et al. 2019. Nat Commun. 10:2225. PubMed Levy BD, et al. 2020. J Allergy Clin Immunol. 145:335. PubMed Flietner E, et al. 2022. Sci Rep. 12:10616. PubMed Pan S, et al. 2021. Front Cell Dev Biol. 9:629306. PubMed Fernández-Orth J, et al. 2020. Eur J Immunol. . PubMed Akiel M, et al. 2017. Cancer Res. 77:4014. PubMed von Roemeling CA, et al. 2020. Nat Commun. 11:1508. PubMed Unsinger J, et al. 2021. J Leukoc Biol. 109:697. PubMed Brownlie D, et al. 2021. J Immunother Cancer. 9:. PubMed Gaspar M, et al. 2020. Cancer Immunol Res. 0.875694444. PubMed Tilley S, et al. 2007. J Immunol . 178:3208. PubMed Kang LJ, et al. 2020. Sci Rep. 10:5603. PubMed Liu J, et al. 2022. Stem Cell Res Ther. 13:247. PubMed Link CWM, et al. 2020. Front Immunol. 11:596772. PubMed Di Dalmazi G, et al. 2019. J Immunol. 202:1350. PubMed Lee YJ, et al. 2018. Front Microbiol. 9:83. PubMed Mohrin M, et al. 2021. Aging Cell. 20:e13313. PubMed Heib T, et al. 2021. Cell Reports. 35(6):109102. PubMed He Y, et al. 2021. eLife. 10:00. PubMed Englezou PC, et al. 2018. Mol Ther Nucleic Acids. 12:118. PubMed Katsuyama T, et al. 2019. J Clin Invest. 129:5411. PubMed Yamashita M, et al. 2019. Cell Stem Cell. 25:357. PubMed Diao J, et al. 2018. J Immunol. 201:1306. PubMed Pushalkar S, et al. 2018. Cancer Discov. 0.613194444. PubMed Gonzales C, et al. 2016. PLoS One. 11:e0164557. PubMed Zhang J, et al. 2019. Onco Targets Ther. 12:4985. PubMed Rastelli D, et al. 2021. J Clin Invest. :e150789. PubMed Moretti FA, et al. 2021. Sci Rep. 11:23221. PubMed Song M, et al. 2020. Nat Commun. 11:6298. PubMed Robles-Valero J, et al. 2021. EMBO J. 40:e108125. PubMed Lei F, et al. 2012. J Vis Exp. 63: 3986. PubMed Yadav A, et al. 2009. Am J Physiol Renal Physiol. 298:734. PubMed Shao Y, et al. 2017. Onco Targets Ther. 10:2675. PubMed Egaña–Gorroño L, et al. 2019. Atherosclerosis. 289:184. PubMed Regan–Komito D, et al. 2017. Front Immunol. 1.459027778. PubMed He X, et al. 2017. Cancer Biol Ther. 0.815277778. PubMed Kästele V, et al. 2021. Mucosal Immunol. 14:717. PubMed
RRID: AB_312658 (BioLegend Cat. No. 100201) AB_312659 (BioLegend Cat. No. 100202)
Structure: Ig superfamily, CD3/TCR, 20 kD
Distribution: Thymocytes (differentiation dependent), mature T cells, NK-T cells
Function: Antigen recognition, TCR signal transduction, T cell activation
Ligand/Receptor: Peptide antigen/MHC-complex
Antigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Davis MM. 1990. Annu. Rev. Biochem. 59:475. 3. Weiss A, et al. 1994. Cell 76:263.
Gene ID: 12502
UniProt: View information about CD3 on UniProt.org
Clone: 17A2
Regulatory Status: RUO
Other Names: T cell antigen receptor complex, T3
Isotype: Rat IgG2b, κ