Biolegend, 100216, Alexa Fluor® 700 anti-mouse CD3 Antibody, 100μg

CD3, also known as T3, is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 is composed of CD3ε, δ, γ and ζ chains. It forms a TCR complex by associating with TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: γδTCR-positive T-T hybridoma D1
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The CD3 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported application (for relevant formats) include: spatial biology (IBEX)1,2.
Application References(PubMed link indicates BioLegend citation): Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations: Hirai T, et al. 2020. Immunity. 54(1):84-98.e5. PubMed Cignarella F et al. 2018. Cell metabolism. 27(6):1222-1235 . PubMed Kwong Chung CK, et al. 2017. Immunology. 150:221. PubMed Ou W, et al. 2023. Nat Commun. 14:392. PubMed Ke F, et al. 2023. Elife. 12:. PubMed Saha S, et al. 2023. Sci Rep. 13:4609. PubMed Ozga AJ, et al. 2022. Immunity. 55:82. PubMed Chae CS, et al. 2022. Cancer Discov. 12:1904. PubMed Steudler J, et al. 2022. Glia. 70:2045. PubMed Gary EN, et al. 2022. Cell Rep Med. 3:100693. PubMed Giannou AD, et al. 2023. Immunity. 56:125. PubMed Briukhovetska D, et al. 2023. Immunity. 56:143. PubMed Feng J, et al. 2022. Immunity. 55:405. PubMed Abdul-Careem MF, et al. 2012. PLoS One. 7:e32821. PubMed Wojno E, et al. 2011. J Immunol. 187:266. PubMed Roco JA et al. 2019. Immunity. 51(2):337-350 . PubMed Hirata Y et al. 2018. Cell stem cell. 22(3):445-453 . PubMed Groenen AG, et al. 2022. J Lipid Res. 63:100167. PubMed Fisher E, et al. 2021. Front Immunol. 11:602254. PubMed Turner JS et al. 2018. Cell reports. 25(6):1395-1403 . PubMed Delás MJ, et al. 2019. Cell Rep. 27:719. PubMed Jiang W, et al. 2022. Front Immunol. 13:878832. PubMed Toomer G, et al. 2022. Viruses. 14:. PubMed Chow MT et al. 2019. Immunity. 50(6):1498-1512 . PubMed Zhang C, et al. 2020. Cell Metabolism. 31(1):148-161.e5.. PubMed Zhang H, et al. 2020. Cancer Cell. 37(1):37-54.e9.. PubMed Ma YV, et al. 2021. MAbs. 13:2003281. PubMed Teng ZX, et al. 2022. J Inflamm Res. 15:3187. PubMed Yu M, et al. 2021. J Clin Invest. 131:. PubMed Yang Y, et al. 2021. Nat Commun. 12:525. PubMed Gary EN, et al. 2021. iScience. 24(7):102699. PubMed Willingham SB, et al. 2018. Cancer Immunol Res. 6:1136. PubMed Jiang H, et al. 2021. Oncoimmunology. 10:1943180. PubMed Leonard JD et al. 2017. Immunity. 47(1):107-117 . PubMed Miska J et al. 2019. Cell reports. 27(1):226-237 . PubMed Watanabe K, et al. 2018. JCI Insight. 3. PubMed Zhang L, et al. 2021. Methods Mol Biol. 2388:175. PubMed Levescot A, et al. 2021. J Clin Invest. 131:. PubMed Everard A, et al. 2014. Nat Commun. 5:5648. PubMed Tyagi AM, et al. 2021. eLife. 10:00. PubMed Leylek R, et al. 2019. Cell Rep. 29:3736. PubMed Progatzky F, et al. 2021. Nature. 599:125. PubMed Gary E, et al. 2020. Vaccine. 3821:38. PubMed Li Q, et al. 2019. Front Pharmacol. 10:786. PubMed , et al. 2021. Eur J Immunol. 51:2708. PubMed Zeng Q, et al. 2022. Front Immunol. 13:740805. PubMed Reese B, et al. 2020. J Immunol. 205:3218. PubMed La Rose AM, et al. 2021. Mol Metab. 53:101265. PubMed Sharma M, et al. 2020. Circ Res. 127:335. PubMed Li A, et al. 2020. J Virol. 94:00:00. PubMed Kaur A, et al. 2019. Cancer Discov. 9:64. PubMed Kiniry BE, et al. 2018. J Immunol. 200:1876. PubMed Liu S, et al. 2020. Cell Host & Microbe. 26(6):779-794.e8.. PubMed Kuhn JA, et al. 2021. Elife. 10:. PubMed Green WD, et al. 2022. J Leukoc Biol. 111:147. PubMed Wu S, et al. 2022. BMC Immunol. 23:17. PubMed Mantilla-Rojas C, et al. 2022. Cancers (Basel). 14:. PubMed Bendtsen SK, et al. 2022. Oncoimmunology. 11:2026020. PubMed Koelwyn GJ, et al. 2020. Nat Med. 1452:26. PubMed Bruder J, et al. 2017. Mol Ther Methods Clin Dev. 10.1016/j.omtm.2017.08.003. PubMed Echevarría-Vargas IM, et al. 2018. EMBO Mol Med. 10:e8446. PubMed Toshiro Hirai et al. 2019. Immunity. 50(5):1249-1261 . PubMed Moguche AO et al. 2017. Cell host & microbe. 21(6):695-706 . PubMed Wuggenig P, et al. 2020. Commun Biol. 3:130. PubMed Ryan NM, et al. 2022. Front Immunol. 13:932742. PubMed Gomez S, et al. 2022. J Immunother Cancer. 10:. PubMed Dulken BW, et al. 2019. Nature. 571:205. PubMed Williams JB, et al. 2020. Nat Commun. 0.876388889. PubMed Rosenbaum SR, et al. 2020. Cell Rep. 30:510. PubMed Godwin MS, et al. 2019. JCI Insight. 4:e126070. PubMed Dahlgren MW et al. 2019. Immunity. 50(3):707-722 . PubMed Agashe VV, et al. 2018. J Immunol. 201:772. PubMed Zeis P, et al. 2020. Immunity. 53:775. PubMed Jaako P, et al. 2016. Leukemia. 10.1038/leu.2016.159. PubMed Moufarrij S, et al. 2020. Sci Rep. 10:3470. PubMed Muchenditsi A, et al. 2017. Am J Physiol Gastrointest Liver Physiol. 313:G39. PubMed Contreras NA, et al. 2019. PLoS Pathog. 15:e1007890. PubMed Zaitseva M, et al. 2017. Antiviral Research. 10.1016/j.antiviral.2017.05.002. PubMed Tulyeu J, et al. 2021. Curr Protoc. 1:e283. PubMed Godwin MS, et al. 2021. Am J Physiol Lung Cell Mol Physiol. 320:L393. PubMed Di Pilato M, et al. 2021. Cell. 184(17):4512-4530.e22. PubMed Lang JF et al. 2019. Nat Commun. 10(1):3415 . PubMed Voeller J, et al. 2019. J Immunother Cancer. 0.530555556. PubMed Chao JL, et al. 2021. Cell Rep Med. 2:100399. PubMed Fedele C, et al. 2021. J Exp Med. 218: . PubMed Mykkänen O, et al. 2014. PLoS One. 9:114790. PubMed Platteel ACM, et al. 2018. Front Immunol. 1.544444444. PubMed Li K, et al. 2020. Nat Commun. 0.795138889. PubMed Säwen P et al. 2018. eLife. 7 pii: e41258. PubMed Sharma M, et al. 2019. Immunometabolism. 1. PubMed Seenappa LM, et al. 2022. NPJ Vaccines. 7:128. PubMed Schulze J, et al. 2021. Stroke. 52:2939. PubMed Spencer AJ, et al. 2022. EBioMedicine. 77:103902. PubMed Chavez JS, et al. 2022. Cells. 11:. PubMed Kong IY, et al. 2020. Cell Rep. 33:108290. PubMed Goldberg EL, et al. 2021. Cell Metabolism. :. PubMed Hebbandi Nanjundappa R, et al. 2017. Cell. 171:655. PubMed Rezende RM, et al. 2019. J Immunol. 203:2621. PubMed Parigger T, et al. 2021. Int J Mol Sci. 22:. PubMed Hendin HE, et al. 2022. NPJ Vaccines. 7:42. PubMed Ye Y, et al. 2020. Genome Med. 0.557638889. PubMed Samuelson DR, et al. 2021. Commun Biol. 4:997. PubMed Jiang W, et al. 2021. Cell Rep. 37:110112. PubMed Chang MH, et al. 2021. Cell Rep. 37:109902. PubMed Gu Z, et al. 2021. Nat Genet. 53:672. PubMed
RRID: AB_493696 (BioLegend Cat. No. 100215) AB_493697 (BioLegend Cat. No. 100216)
Structure: Ig superfamily, CD3/TCR, 20 kD
Distribution: Thymocytes (differentiation dependent), mature T cells, NK-T cells
Function: Antigen recognition, TCR signal transduction, T cell activation
Ligand/Receptor: Peptide antigen/MHC-complex
Antigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Davis MM. 1990. Annu. Rev. Biochem. 59:475. 3. Weiss A, et al. 1994. Cell 76:263.
Gene ID: 12502
UniProt: View information about CD3 on UniProt.org
Clone: 17A2
Regulatory Status: RUO
Other Names: T cell antigen receptor complex, T3
Isotype: Rat IgG2b, κ