Biolegend, 100246, PE/Dazzle™ 594 anti-mouse CD3 Antibody, 100μg

CD3, also known as T3, is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 is composed of CD3ε, δ, γ and ζ chains. It forms a TCR complex by associating with TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: γδTCR-positive T-T hybridoma D1
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Dazzle™ 594 under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * PE/Dazzle™ 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported application (for relevant formats) include: spatial biology (IBEX)1,2.
Application References(PubMed link indicates BioLegend citation): Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations: Bajaj R, et al. 2022. Cell Rep. 40:111429. PubMed Krummey SM, et al. 2020. Cell Reports. 30(5):1282-1291.e5.. PubMed Ma F, et al. 2020. Cell Death Dis. 1.172222222. PubMed Schmidleithner L et al. 2019. Immunity. 50(5):1232-1248 . PubMed Starkl P, et al. 2020. Immunity. 53(4):793-804.e9. PubMed Guo HF, et al. 2021. Commun Biol. 4:482. PubMed Paiva RS, et al. 2021. Eur J Immunol. 51:1968. PubMed He W et al. 2018. Immunity. 49(6):1175-1190 . PubMed Taniguchi H, et al. 2022. Cell Rep. 39:110814. PubMed Lu X, et al. 2020. Sci Transl Med. 12:. PubMed Lu S, et al. 2020. Arterioscler Thromb Vasc Biol. 40:394. PubMed Liang J, et al. 2020. Sci Adv. 6:eabc3646. PubMed Tan X, et al. 2021. Sci Adv. 7: . PubMed Padhye A, et al. 2019. Sci Rep. 9:4819. PubMed
RRID: AB_2565882 (BioLegend Cat. No. 100245) AB_2565883 (BioLegend Cat. No. 100246)
Structure: Ig superfamily, CD3/TCR, 20 kD
Distribution: Thymocytes (differentiation dependent), mature T cells, NK-T cells
Function: Antigen recognition, TCR signal transduction, T cell activation
Ligand/Receptor: Peptide antigen/MHC-complex
Antigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Davis MM. 1990. Annu. Rev. Biochem. 59:475. 3. Weiss A, et al. 1994. Cell 76:263.
Gene ID: 12502
UniProt: View information about CD3 on UniProt.org
Clone: 17A2
Regulatory Status: RUO
Other Names: T cell antigen receptor complex, T3
Isotype: Rat IgG2b, κ