Biolegend, 100307, PE anti-mouse CD3ε Antibody, 50μg

CD3ε is a 20 kD transmembrane protein, also known as CD3 or T3. It is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3ε forms a TCR complex by associating with the CD3δ, γ and ζ chains, as well as the TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex.
50μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Armenian Hamster
Immunogen: H-2Kb-specific mouse cytotoxic T lymphocyte clone BM10-37
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Clone 145-2C11 is useful for in vitro blocking of target-specific CTL-mediated cell lysis1, as well as T cell activation assays, inducing proliferation and cytokine production1,2,7,12,16. It also induces apoptosis in immature thymocytes32, and in vivo T cell depletion8-10. Additional reported applications (for relevant formats of this clone) include: immunoprecipitation1, immunohistochemical staining14,15 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, Western blotting4, complement-mediated cytotoxicity6, in vitro and in vivo stimulation of T cells1,2,7,12,16, immunofluorescent staining5, and in vivo T cell depletion8-10. The 145-2C11 antibody has been reported to block the binding of 17A2 antibody to CD3 epsilon-specific T cells11. Clone 145-2C11 is not recommended for formalin-fixed paraffin embedded sections. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 100314). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 100340) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
Application References(PubMed link indicates BioLegend citation): Leo O, et al. 1987. P. Natl. Acad. Sci. USA 84:1374. (IP, Activ, Block) Kruisbeek AM, et al. 1991. In Current Protocols in Immunology. 3.12.1. (Activ) Duke RC, et al. 1995. Current Protocols in Immunology. 3.17.1. Salvadori S, et al. 1994. J. Immunol. 153:5176. (WB) Payer E, et al. 1991. J. Immunol. 146:2536. (IF) Jacobs H, et al. 1994. Eur. J. Immunol. 24:934. (CMCD) Vossen ACTM, et al. 1995. Eur. J. Immunol. 25:1492. (Activ) Henrickson M, et al. 1995. Transplantation 60:828. (Deplete) Kinnaert P, et al. 1996. Transpl. Int. 9:386. (Deplete) Han WR, et al. 1999. Transpl. Immunol. 7:207. (Deplete) Miescher GC, et al. 1989. Immunol. Lett. 23:113. (Block) Terrazas LI, et al. 2005. Intl. J. Parasitology. 35:1349. (Activ) Ko SY, et al. 2005. J. Immunol. 175:3309. Podd BS, et al. 2006. J. Immunol. 176:6532. (IHC-F) Tilley SL, et al. 2007. J. Immunol. 178:3208. (IHC-F) Wang W, et al. 2007. J. Immunol. 178:4885. (Activ) Xiao S, et al. 2007. J. Exp. Med. 204:1691. Chappaz S, et al. 2007. Blood doi:10.1182/blood-2007-02-074245. (FC) PubMed. Curtsinger JM, et al.2005. J. Immunol. 175:4392. PubMed Guo Y, et al. 2008. Blood 112:480. PubMed Kenna TJ, et al. 2008. Blood 111:2091. Perchonock CE, et al. 2007. J. Immunol. 179:1768. PubMed Perchonock GE, et al. 2006. Mol. Cell. Biol. 26:6005. PubMed Kanaya T, et al. 2008. Am. J. Physiol. Gastrointest. Liver Physiol. 295:G273. PubMed de Koning BA, et al. 2006. Int. Immunol. 18:941. PubMed Schulteis RD, et al. 2008. Blood 295:G273. PubMed Qi Q, et al. 2009. Blood 114:564. PubMed Helmersson S, et al. 2013. Am J Pathol. 9440:123. Pubmed Wu S, et al. 2014. Clin Vaccine Immunol. 21:156. PubMed Yan J, et al. 2014. Vaccine. 32:2833. PubMed Guiterrez DA, et al. 2014. Diaebetes. 63:3827. PubMed Shi YF, et al. 1991. J Immunol. 146:3340. (Apop)
Product Citations: Guo H, Cooper S, Friedman A, et al. 2017. PLoS One. 10.1371/journal.pone.0150809. PubMed Chang YS, et al. 2020. Int J Mol Sci. 21:00. PubMed Logan K Smith et al. 2018. Immunity. 48(2):299-312 . PubMed Wu J et al. 2017. Immunity. 47(6):1114-1128 . PubMed Jayachandran R, et al. 2019. Immunity. 50:152. PubMed Sierro F, et al. 2017. Immunity. 47:374. PubMed Yu X, et al. 2020. Nat Commun. 11:1110. PubMed Olofsen PA, et al. 2022. Cells. 11:. PubMed Wu Y, et al. 2023. Nat Cancer. 4:382. PubMed Sambandam A, et al. 2023. Heliyon. 9:e14238. PubMed Touré H, et al. 2023. PLoS Pathog. 19:e1011257. PubMed He Y, et al. 2023. Biomolecules. 13:. PubMed Gao Y, et al. 2023. iScience. 26:106729. PubMed Grigsby SM, et al. 2021. Cancers (Basel). 13:. PubMed Wang L, et al. 2022. ACS Pharmacol Transl Sci. 5:110. PubMed Iberg CA, et al. 2022. Cell Rep. 39:110657. PubMed Ocaña-Guzman R, et al. 2022. Cells. 11: . PubMed Wang R, et al. 2023. NPJ Vaccines. 8:6. PubMed Zhao K, et al. 2023. Front Immunol. 14:1101769. PubMed Hajjar S, et al. 2022. Cell Death Differ. 29:585. PubMed Ramdas B, et al. 2022. Mol Ther. 30:2505. PubMed Zong P, et al. 2022. Nat Cardiovasc Res. 1:344. PubMed Elazar A, et al. 2022. Elife. 11:. PubMed Thelin MA, et al. 2017. Diabetes. 66:2220. PubMed Taddeo A, et al. 2022. NPJ Vaccines. 7:82. PubMed MacDonald K, et al. 2014. J Immunol. 192:3180. PubMed Grødeland G, et al. 2020. Front Immunol. 11:431. PubMed Piepke M, et al. 2021. J Neuroinflammation. 18:265. PubMed Qin Y, et al. 2021. Cell Rep. 37:109968. PubMed Acharya N, et al. 2020. Immunity. 53(3):658-671.e6. PubMed Hiraishi Y, et al. 2018. Sci Rep. 8:18052. PubMed Leier A, et al. 2022. Mol Ther Nucleic Acids. 28:261. PubMed Motozono C, et al. 2014. Microbes Infect. 16:320. PubMed D Kim, S Lee, H Jun 2016. Immunol Cell Biol. 10.1038/icb.2016.98. PubMed Okuniewska M, et al. 2021. Cell Reports. 36(2):109368. PubMed Bartleson JM, et al. 2020. Nat Immunol. 1384:21. PubMed Dave K et al. 2017. eLife. 6 pii: e23382. PubMed LaFleur MW, et al. 2019. Nat Immunol. 20:1335. PubMed Wang G, et al. 2021. Nat Commun. 12:5733. PubMed Vazquez S, et al. 2015. Exp Hematol. 43: 578-585. PubMed Sarapulov AV, et al. 2020. Front Immunol. 11:599. PubMed Pan W et al. 2017. Immunity. 47(2):284-297 . PubMed Xu X, et al. 2021. J Nanobiotechnology. 19:376. PubMed Vitoriano-Souza J, et al. 2012. PLoS One. 7:e40745. PubMed Zhang B, et al. 2016. Mol Cell. 63: 976-89. PubMed Davidson S, et al. 2020. Cell Reports. 31(7):107628. PubMed Zhang B, et al. 2022. J Exp Med. 219:. PubMed Yoshida K, et al. 2022. Sci Rep. 12:17276. PubMed Flommersfeld S, et al. 2021. Immunity. :. PubMed Qiu Z, et al. 2016. Sci Rep. 6:31936. PubMed Gaudreault N, et al. 2013. Am J Pathol. 2:605. PubMed He L, et al. 2019. iScience. 0.790972222. PubMed Kotaki R, et al. 2020. Sci Rep. 10:13554. PubMed Ma J, et al. 2016. Proc Natl Acad Sci U S A. 113: 450 - 458. PubMed Ruer-Laventie J, et al. 2020. Bio Protoc. e3531:10. PubMed Wen Y, et al. 2020. Hypertension. 869:75. PubMed Ringel AE, et al. 2020. Cell. 183(7):1848-1866.e26. PubMed Siamishi I, et al. 2020. Cell Reports. 31(11):107756. PubMed Leal AS, et al. 2019. Sci Rep. 5.286111111. PubMed Qi S et al. 2016. eLife. 5 pii: e14756. PubMed Malik A et al. 2018. Immunity. 49(3):515-530 . PubMed Boulay A, et al. 2015. J Neurosci. 35:4427. PubMed Faust HJ, et al. 2020. J Clin Invest. 130:5493. PubMed Abe M, et al. 2020. Clin Exp Immunol. 303:199. PubMed LaFleur MW, et al. 2019. Nat Commun. 10:1668. PubMed Bourque J, et al. 2021. Heliyon. 7:e08311. PubMed Park HB, et al. 2020. Nat Microbiol. 1319:5. PubMed Kim P, et al. 2015. Infect Immun . 83: 2992 - 3002. PubMed Guo Y, et al. 2013. Infect Immun . 81:3923. PubMed Kretschmer L, et al. 2020. Nat Commun. 0.536805556. PubMed Chulpanova DS, et al. 2021. Biology (Basel). 10:. PubMed Chen RJ, et al. 2022. iScience. 25:105595. PubMed Ha TC, et al. 2020. Hum Gene Ther. 32:458. PubMed Abdelfattah N, et al. 2022. Nat Commun. 13:767. PubMed Shibuya M, et al. 2021. iScience. 24:103131. PubMed Ben-Yehuda H, et al. 2021. Mol Neurodegener. 16:39. PubMed Martin R, et al. 2014. PLoS One. 9:110609. PubMed Cecchinato V, et al. 2017. J Immunol. 198(1):184-195. PubMed Gomzikova MO, et al. 2020. Pharmaceutics. 12:00. PubMed Kristensen LK, et al. 2020. Mol Imaging Biol. 1.625694444. PubMed Wang L, et al. 2021. Sci Adv. 7:eabj4796. PubMed Unsinger J, et al. 2021. J Leukoc Biol. 109:697. PubMed Mah-Som AY, et al. 2021. Cell Reports. 35(9):109209. PubMed Bergquist M, et al. 2014. J Infect. 69:113. PubMed Kuo P, et al. 2016. J Am Heart Assoc. 5: 002610. PubMed Ji XJ, et al. 2019. Mediators Inflamm. 2019:2750528. PubMed Celis‐Gutierrez J et al. 2019. Cell Rep. 27(11):3315-3330 . PubMed Mohrin M, et al. 2021. Aging Cell. 20:e13313. PubMed Hara T, et al. 2021. Cancer Cell. 39(6):779-792.e11. PubMed Hu KH, et al. 2020. Nat Methods. 1.286805556. PubMed Simoni L, et al. 2020. Cell Rep. 33:108330. PubMed Celià-Terrassa T, et al. 2017. Nat Cell Biol. 19:711. PubMed Tang-Huau TL, et al. 2021. Viruses. 13: . PubMed Bansal K, et al. 2017. Nat Immunol. 18:263-273. PubMed Evavold CL, et al. 2021. Cell. 184(17):4495-4511.e19. PubMed Dyer DP et al. 2019. Immunity. 50(2):378-389 . PubMed Dourcy M, et al. 2020. Mucosal Immunol. 13:799. PubMed Avgustinova A, et al. 2021. Cell Stem Cell. . PubMed Campisi L, et al. 2016. Nat Immunol. 10.1038/ni.3512. PubMed Qi S, et al. 2020. Theranostics. 10:1814. PubMed Kim I et al. 2015. Brain and behavior. 5(12):e00403 . PubMed Kubli SP, et al. 2019. Nat Commun. 10:2678. PubMed Sethuraman SN, et al. 2020. Theranostics. 10:3397. PubMed Artinger M, et al. 2021. Int J Mol Sci. 22: . PubMed
RRID: AB_312672 (BioLegend Cat. No. 100307) AB_312673 (BioLegend Cat. No. 100308)
Structure: Ig superfamily, forms CD3/TCR complex with CD3δ, γ and ζ subunits and TCR (α/β and γ/δ), 20 kD
Distribution: Thymocytes (differentiation dependent), mature T cells, NK-T cells
Function: TCR signal transduction, T cell activation, antigen recognition
Ligand/Receptor: Peptide antigen/MHC-complex
Cell Type: NKT cells, T cells, Thymocytes, Tregs
Biology Area: Immunology
Molecular Family: CD Molecules, TCRs
Antigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Davis MM. 1990. Annu. Rev. Biochem. 59:475. 3. Weiss A, et al. 1994. Cell 76:263.
Gene ID: 12501
UniProt: View information about CD3epsilon on UniProt.org
Clone: 145-2C11
Regulatory Status: RUO
Other Names: CD3ε, T3, CD3
Isotype: Armenian Hamster IgG
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.