Biolegend, 100603, Biotin anti-mouse CD5 Antibody, 50μg

CD5 is a 67 kD protein, also known as Lyt-1, Ly-1, T1, Tp67, or Ly-12. It is a member of the scavenger receptor cysteine-rich protein superfamily (SRCR) and primarily expressed on thymocytes, T cells, and B-1 cells. Although mature α/β T cells express high levels of CD5, very few γ/δ T cells express this antigen. The interaction of CD5 with CD72, gp35-37, TCR, or BCR is involved in T and B cell activation.
50μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse thymus or spleen
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.06 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation1, and immunohistochemistry2 of acetone-fixed frozen tissue sections, zinc-fixed paraffin-embedded sections and formalin-fixed paraffin-embedded sections.
Application References(PubMed link indicates BioLegend citation): Ledbetter JA, et al. 1979. Immunol. Rev. 47:63. (IP) Ledbetter JA, et al. 1980. J. Exp. Med. 152:280. (FC, IHC) Bourdeau A, et al. 2007. Blood doi:10.1182/blood-2006-08-044370.
Product Citations: Mirchandani AS, et al. 2022. Nat Immunol. 23:927. PubMed Tomiaki C, et al. 2022. Front Immunol. 13:1014462. PubMed Giannou AD, et al. 2023. Immunity. 56:125. PubMed Wu J, et al. 2022. Nat Commun. 13:7321. PubMed Sasse C, et al. 2022. Pathogens. 11:. PubMed Park JG, et al. 2021. iScience. 24(9):102941. PubMed Yamamoto K, et al. 2021. Cell Reports. 36(8):109576. PubMed Li Q et al. 2018. Immunity. 48(2):258-270 . PubMed Fukushima T, et al. 2019. Cell Rep. 29:4144. PubMed Dey A et al. 2019. The EMBO journal. 38(7) pii: e100293. PubMed She L, et al. 2021. JCI Insight. 6:e143509. PubMed He J, et al. 2020. Cell Reports. 29(9):2718-2730.e6.. PubMed Hurrell BP, et al. 2019. Cell Rep. 29:4509. PubMed Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed Comazzetto S et al. 2018. Cell stem cell. 24(3):477-486 . PubMed Zhang Z, et al. 2019. Haematologica. 4.339583333. PubMed Galle-Treger L, et al. 2020. J Allergy Clin Immunol. 145:502. PubMed Zeis P, et al. 2020. Immunity. 53:775. PubMed Rohrbeck L, et al. 2020. Immunol Cell Biol. . PubMed Bajaña S, et al. 2022. iScience. 25:103732. PubMed Miyauchi K, et al. 2021. Nat Commun. 12:3789. PubMed Mohrin M, et al. 2021. Aging Cell. 20:e13313. PubMed Rossnagl S, et al. 2016. PLoS Biol. 14: 1002562. PubMed Young K, et al. 2021. Cell Stem Cell. . PubMed
RRID: AB_312732 (BioLegend Cat. No. 100603) AB_312733 (BioLegend Cat. No. 100604)
Structure: Member of the scavenger receptor cysteine-rich protein superfamily (SRCR), 67 kD
Distribution: Thymocytes, T cells, B-1 cells
Function: Negative regulator of T-B cell interaction
Ligand/Receptor: CD72, gp35-37
Cell Type: B cells, T cells, Thymocytes
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Kipps TJ. 1988. Adv. Immunol. 47:117. 3. Antin JH, et al. 1985. J. Immunol. 136:505. 4. Tarakhovsky A, et al. 1995. Science 269:535.
Gene ID: 12507
UniProt: View information about CD5 on UniProt.org
Clone: 53-7.3
Regulatory Status: RUO
Other Names: Lyt-1, Ly-1, T1, Tp67, Ly-12
Isotype: Rat IgG2a, κ
Q: How many biotin molecules are per antibody structure?
A: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.