Biolegend, 100614, Alexa Fluor® 647 anti-mouse CD5 Antibody, 100μg

CD5 is a 67 kD protein, also known as Lyt-1, Ly-1, T1, Tp67, or Ly-12. It is a member of the scavenger receptor cysteine-rich protein superfamily (SRCR) and primarily expressed on thymocytes, T cells, and B-1 cells. Although mature α/β T cells express high levels of CD5, very few γ/δ T cells express this antigen. The interaction of CD5 with CD72, gp35-37, TCR, or BCR is involved in T and B cell activation.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse thymus or spleen
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation1, and immunohistochemistry2 of acetone-fixed frozen tissue sections, zinc-fixed paraffin-embedded sections and formalin-fixed paraffin-embedded sections.
Application References(PubMed link indicates BioLegend citation): Ledbetter JA, et al. 1979. Immunol. Rev. 47:63. (IP) Ledbetter JA, et al. 1980. J. Exp. Med. 152:280. (FC, IHC) Bourdeau A, et al. 2007. Blood doi:10.1182/blood-2006-08-044370.
Product Citations: Kaku H, et al. 2014. J Immunol. 193:5904. PubMed Piper CJM, et al. 2020. Cell Reports. 29(7):1878-1892.e7.. PubMed Lopez DA, et al. 2022. Cell Rep. 41:111677. PubMed Rosser EC, et al. 2020. Cell Metabolism. 31(4):837-851. PubMed Smith LK, et al. 2021. Elife. 10:. PubMed
RRID: AB_493168 (BioLegend Cat. No. 100613) AB_2075301 (BioLegend Cat. No. 100614)
Structure: Member of the scavenger receptor cysteine-rich protein superfamily (SRCR), 67 kD
Distribution: Thymocytes, T cells, B-1 cells
Function: Negative regulator of T-B cell interaction
Ligand/Receptor: CD72, gp35-37
Cell Type: B cells, T cells, Thymocytes
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Kipps TJ. 1988. Adv. Immunol. 47:117. 3. Antin JH, et al. 1985. J. Immunol. 136:505. 4. Tarakhovsky A, et al. 1995. Science 269:535.
Gene ID: 12507
UniProt: View information about CD5 on UniProt.org
Clone: 53-7.3
Regulatory Status: RUO
Other Names: Lyt-1, Ly-1, T1, Tp67, Ly-12
Isotype: Rat IgG2a, κ