Biolegend, 100624, PerCP/Cyanine5.5 anti-mouse CD5 Antibody, 100μg

CD5 is a 67 kD protein, also known as Lyt-1, Ly-1, T1, Tp67, or Ly-12. It is a member of the scavenger receptor cysteine-rich protein superfamily (SRCR) and primarily expressed on thymocytes, T cells, and B-1 cells. Although mature α/β T cells express high levels of CD5, very few γ/δ T cells express this antigen. The interaction of CD5 with CD72, gp35-37, TCR, or BCR is involved in T and B cell activation.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse thymus or spleen
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation1, and immunohistochemistry2 of acetone-fixed frozen tissue sections, zinc-fixed paraffin-embedded sections and formalin-fixed paraffin-embedded sections.
Application References(PubMed link indicates BioLegend citation): Ledbetter JA, et al. 1979. Immunol. Rev. 47:63. (IP) Ledbetter JA, et al. 1980. J. Exp. Med. 152:280. (FC, IHC) Bourdeau A, et al. 2007. Blood doi:10.1182/blood-2006-08-044370.
Product Citations: Harsha Krovi S, et al. 2020. Nat Commun. 4.790277778. PubMed Simpson AP, et al. 2022. Cell Rep. 40:111099. PubMed Frisbee AL, et al. 2019. Nat Commun. 10:2712. PubMed Català C, et al. 2022. iScience. 25:105078. PubMed Kobayashi T, et al. 2019. Cell. 176:982. PubMed Fachi JL, et al. 2020. J Exp Med. 217:00:00. PubMed Bennion BG, et al. 2020. Cell Reports. 31(11):107771. PubMed Snyder LM, et al. 2022. Immunohorizons. 6:660. PubMed Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed McFarland AP, et al. 2021. Immunity. 54(6):1320-1337.e4. PubMed Uddin MJ, et al. 2022. Mucosal Immunol. 15:165. PubMed Gomez S, et al. 2022. J Immunother Cancer. 10:. PubMed Sakamoto K, et al. 2021. Immunity. 54:2321. PubMed Nasarre P, et al. 2021. Cancers (Basel). 13: . PubMed Kunishita Y, et al. 2020. Front Immunol. 11:98. PubMed Peng V, et al. 2022. Nat Immunol. 23:619. PubMed Teng F, et al. 2021. Cell Rep. 37:110051. PubMed Sakamoto K, et al. 2022. STAR Protoc. 3:101052. PubMed Borrelli MR, et al. 2020. Stem Cells Transl Med. 1.347916667. PubMed Goc J, et al. 2021. Cell. 184:5015. PubMed
RRID: AB_2563432 (BioLegend Cat. No. 100623) AB_2563433 (BioLegend Cat. No. 100624)
Structure: Member of the scavenger receptor cysteine-rich protein superfamily (SRCR), 67 kD
Distribution: Thymocytes, T cells, B-1 cells
Function: Negative regulator of T-B cell interaction
Ligand/Receptor: CD72, gp35-37
Cell Type: B cells, T cells, Thymocytes
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Kipps TJ. 1988. Adv. Immunol. 47:117. 3. Antin JH, et al. 1985. J. Immunol. 136:505. 4. Tarakhovsky A, et al. 1995. Science 269:535.
Gene ID: 12507
UniProt: View information about CD5 on UniProt.org
Clone: 53-7.3
Regulatory Status: RUO
Other Names: Lyt-1, Ly-1, T1, Tp67, Ly-12
Isotype: Rat IgG2a, κ
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.