Biolegend, 101604, Biotin anti-mouse CD23 Antibody, 500μg

CD23 is a 45 kD protein also known as low affinity IgE Fc receptor, FcεRII, BLAST-2, Ly-42, or B6. It is a member of the Ig family, expressed on conventional B (but not B-1) cells and follicular dendritic cells. CD23 responds to high levels of IgE by downregulating IgE secretion.
500μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Complex of IgE with Fcε receptor isolated from the mouse B hybridoma cell line O1.2B2
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for other applications.
Application Notes: The B3B4 antibody is useful for blocking IgE activity in vivo. Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunofluorescence microscopy, in vitro and in vivo blocking of ligand binding2-4, immunohistochemical staining of acetone-fixed frozen sections5, and spatial biology (IBEX)7,8.
Application References(PubMed link indicates BioLegend citation): Waldschmidt TJ, et al. 1988. J. Immunol. 140:2148. (IP) Rao M, et al. 1987. J. Immunol. 138:1845. (Block) Oshiba A, et al. 1997. J. Immunol. 159:4056. (Block) Dasic G, et al. 1999. Eur. J. Immunol. 29:2957. (Block) Maeda K, et al. 1992. J. Immunol. 148:2340. (IHC) Craig VJ, et al. 2011. Cancer Res. 71:3616. PubMed Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations: Lino AC et al. 2018. Immunity. 49(1):120-133 . PubMed Yang Y, et al. 2021. Nat Commun. 12:525. PubMed Hrdinka M, et al. 2016. PLoS One. 11: 0162863. PubMed Rohrbeck L, et al. 2020. Immunol Cell Biol. . PubMed Muri J, et al. 2020. Cell Reports. 29(9):2731-2744.e4.. PubMed Werner A, et al. 2021. iScience. 24:103076. PubMed
RRID: AB_312828 (BioLegend Cat. No. 101603) AB_312829 (BioLegend Cat. No. 101604)
Structure: C-lectin family, type II transmembrane glycoprotein, 45 kD
Distribution: B cells, follicular dendritic cells
Function: Regulates B cell activation
Ligand/Receptor: IgE, CD19/CD21/CD81
Cell Type: B cells, Dendritic cells
Biology Area: Immunology
Molecular Family: CD Molecules, Fc Receptors
Antigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Delespesse G, et al. 1992. Immunol. Rev. 125:77. 3. Flores-Romo L, et al. 1993. Science 261:1038.
Gene ID: 14128
UniProt: View information about CD23 on UniProt.org
Clone: B3B4
Regulatory Status: RUO
Other Names: FcεRII, IgE Fc Receptor, BLAST-2, Ly-42, B6
Isotype: Rat IgG2a, κ
Q: How many biotin molecules are per antibody structure?
A: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.