Biolegend, 101613, PE/Cyanine7 anti-mouse CD23 Antibody, 25μg

CD23 is a 45 kD protein also known as low affinity IgE Fc receptor, FcεRII, BLAST-2, Ly-42, or B6. It is a member of the Ig family, expressed on conventional B (but not B-1) cells and follicular dendritic cells. CD23 responds to high levels of IgE by downregulating IgE secretion.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Complex of IgE with Fcε receptor isolated from the mouse B hybridoma cell line O1.2B2
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: The B3B4 antibody is useful for blocking IgE activity in vivo. Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunofluorescence microscopy, in vitro and in vivo blocking of ligand binding2-4, immunohistochemical staining of acetone-fixed frozen sections5, and spatial biology (IBEX)7,8.
Application References(PubMed link indicates BioLegend citation): Waldschmidt TJ, et al. 1988. J. Immunol. 140:2148. (IP) Rao M, et al. 1987. J. Immunol. 138:1845. (Block) Oshiba A, et al. 1997. J. Immunol. 159:4056. (Block) Dasic G, et al. 1999. Eur. J. Immunol. 29:2957. (Block) Maeda K, et al. 1992. J. Immunol. 148:2340. (IHC) Craig VJ, et al. 2011. Cancer Res. 71:3616. PubMed Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations: Meyer SN, et al. 2020. Immunity. 51(3):535-547. PubMed Masle-Farquhar E, et al. 2023. Front Immunol. 14:1095257. PubMed Gómez-Díaz C, et al. 2021. iScience. 24:103241. PubMed Holodick N, et al. 2014. J Immunol. 192:2432. PubMed Onodera T, et al. 2016. J Immunol. 196: 4172 - 4184. PubMed Hutter K, et al. 2020. FEBS J. . PubMed Sangesland M, et al. 2020. Cell Reports. 32(8):108065. PubMed Schoeler K, et al. 2019. FEBS J. 10.1111/febs.14934. PubMed Chiu Y, et al. 2014. J Immunol. 193:2207. PubMed Holodick N, et al. 2016. J Immunol. 196: 4348 - 4357. PubMed Wang YH, et al. 2019. Front Immunol. 10:1909. PubMed Riedel R, et al. 2020. Nat Commun. 11:2570. PubMed Lehrke MJ, et al. 2021. Elife. 10:. PubMed Kimura S, et al. 2020. Am J Transplant. 20:977. PubMed Qiu Z, et al. 2020. Cancer Genet. 245:6. PubMed Fiala G, et al. 2015. J Exp Med. 212: 1693-1708. PubMed Vijay R, et al. 2020. Nat Immunol. 21:790. PubMed Hodgson R, et al. 2022. Commun Biol. 5:1216. PubMed
RRID: AB_2103037 (BioLegend Cat. No. 101613) AB_2103036 (BioLegend Cat. No. 101614)
Structure: C-lectin family, type II transmembrane glycoprotein, 45 kD
Distribution: B cells, follicular dendritic cells
Function: Regulates B cell activation
Ligand/Receptor: IgE, CD19/CD21/CD81
Cell Type: B cells, Dendritic cells
Biology Area: Immunology
Molecular Family: CD Molecules, Fc Receptors
Antigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Delespesse G, et al. 1992. Immunol. Rev. 125:77. 3. Flores-Romo L, et al. 1993. Science 261:1038.
Gene ID: 14128
UniProt: View information about CD23 on UniProt.org
Clone: B3B4
Regulatory Status: RUO
Other Names: FcεRII, IgE Fc Receptor, BLAST-2, Ly-42, B6
Isotype: Rat IgG2a, κ