Biolegend, 101908, FITC anti-mouse CD25 Antibody, 500μg

CD25 is a 55 kD glycoprotein, also known as the low affinity IL-2Rα, Ly-43, p55, or Tac. It is expressed on activated T and B cells, thymocyte subset, pre-B cells, and T regulatory cells. In association with CD122 (IL-2Rβ) and CD132(common γ chain), CD25 forms the high affinity signaling IL-2 receptor.
500μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: IL-2-dependent BALB/c mouse helper T-cell clone HT-2
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Additional reported applications (for the relevant formats) include: in vitro blocking of IL-2 binding to low- and high-affinity receptors1,2, and immunohistochemical staining of acetone-fixed frozen sections. 3C7 antibody recognizes different epitope of PC61 antibody (Cat. No. 102002). The Ultra-LEAF™Purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 101925 and 101926).
Application References(PubMed link indicates BioLegend citation): Ortega RG, et al. 1984. J. Immunol. 133:1970. (Block) Moreau JL, et al. 1987. Eur. J. Immunol. 17:929. (Block)
Product Citations: Santharam MA, et al. 2023. Int J Mol Sci. 24:. PubMed Chen N, et al. 2021. Front Immunol. 12:756423. PubMed Qi X, et al. 2022. FASEB J. 36:e22250. PubMed Zhao Z, et al. 2022. Adv Sci (Weinh). 9:e2201293. PubMed Glaubitz J, et al. 2023. Gut. :. PubMed Krummey SM, et al. 2020. Cell Reports. 30(5):1282-1291.e5.. PubMed Mulder R, et al. 2017. Front Immunol. 1.464583333. PubMed Li Y, et al. 2020. Cell Rep. 30:1753. PubMed Wang Y, et al. 2016. Sci Rep. 6:31881. PubMed Dolgova EV, et al. 2022. Int J Mol Sci. 23:. PubMed Levine LS, et al. 2021. Immunity. 54(4):829-844.e5. PubMed Fujita Y et al. 2018. Cell reports. 24(12):3296-3311 . PubMed Bennion BG, et al. 2020. Cell Reports. 31(11):107771. PubMed Yang K, et al. 2022. J Clin Invest. 132:. PubMed Carbonnelle D, et al. 2009. J Pharmacol Exp Ther. 331:771. PubMed Snell LM, et al. 2018. Immunity. 49:678. PubMed Liu W, et al. 2019. Epigenetics. 1.192361111. PubMed Jiang Y, et al. 2020. Adv Mater. 32:e2001808. PubMed Yan J, et al. 2020. Cell Rep. 107820:31. PubMed Huang J, et al. 2014. J Immunol. 192:1972. PubMed Li D, et al. 2022. Emerg Microbes Infect. 11:2248. PubMed van Geffen C, et al. 2021. Front Immunol. 12:695933. PubMed Shi R, et al. 2020. J Interferon Cytokine Res. 40:188. PubMed Yang FM, et al. 2022. Front Immunol. 13:918241. PubMed
RRID: AB_961210 (BioLegend Cat. No. 101907) AB_961212 (BioLegend Cat. No. 101908)
Structure: Forms high affinity IL-2R with IL-2Rβ (CD122) and IL-2Rγ (CD132), 55 kD
Distribution: Activated T cells and B cells, thymocyte subset, pre-B cells, T regulatory cells
Function: IL-2 receptor, signal transduction
Ligand/Receptor: IL-2
Cell Type: B cells, T cells, Tregs
Biology Area: Immunology
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors
Antigen References: 1. Taniguchi T, et al. 1993. Cell 73:5. 2. Waldmann TA. 1991. J. Biol. Chem. 266:2681. 3. Read S, et al. 2000. J. Exp. Med. 192:295. 4. Lowenthal JW, et al. 1985. J. Immunol. 135:3988.
Gene ID: 16184
UniProt: View information about CD25 on UniProt.org
Clone: 3C7
Regulatory Status: RUO
Other Names: IL-2Rα, Ly-43, p55, Tac
Isotype: Rat IgG2b, κ