Biolegend, 101913, Purified anti-mouse CD25 (Maxpar® Ready) Antibody, 100μg

CD25 is a 55 kD glycoprotein, also known as the low affinity IL-2Rα, Ly-43, p55, or Tac. It is expressed on activated T and B cells, thymocyte subset, pre-B cells, and T regulatory cells. In association with CD122 (IL-2Rβ) and CD132(common γ chain), CD25 forms the high affinity signaling IL-2 receptor.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: IL-2-dependent BALB/c mouse helper T-cell clone HT-2
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 1.0 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested CyTOF® - Verified
Recommended Usage: This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling using Maxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies.
Application Notes: Additional reported applications (for the relevant formats) include: in vitro blocking of IL-2 binding to low- and high-affinity receptors1,2, and immunohistochemical staining of acetone-fixed frozen sections. 3C7 antibody recognizes different epitope of PC61 antibody (Cat. No. 102002). The Ultra-LEAF™Purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 101925 and 101926).
Additional Product Notes: Maxpar® is a registered trademark of Standard BioTools Inc.
Application References(PubMed link indicates BioLegend citation): Ortega RG, et al. 1984. J. Immunol. 133:1970. (Block) Moreau JL, et al. 1987. Eur. J. Immunol. 17:929. (Block)
Product Citations: Kobayashi A, et al. 2021. Front Immunol. 12:650856. PubMed Hezaveh K, et al. 2022. Immunity. 55:324. PubMed Waickman AT, et al. 2017. Cytokine. 99:266. PubMed Wan Mohd Zawawi WFA, et al. 2021. Sci Rep. 11:10278. PubMed Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed Wang W, et al. 2018. Cancer Cell. 34:757. PubMed Wang Y, et al. 2021. Cancer Cell. 39:1375. PubMed Düsedau HP, et al. 2019. Glia. 67:193. PubMed Chen S, et al. 2018. Immunohorizons. 0.345138889. PubMed Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed Zhao L, et al. 2018. Nat Med. 24:1536. PubMed Stacey MA, et al. 2017. J Clin Invest. 127:1463. PubMed Tan Z, et al. 2020. Mol Ther Oncolytics. 16:302. PubMed Zhong X, et al. 2020. Proc Natl Acad Sci U S A. 8563:117. PubMed Crowell PD et al. 2019. Cell Rep. 28(6):1499-1510 . PubMed Jordan S, et al. 2020. Cell. 178(5):1102-1114.e17.. PubMed Scortegagna M, et al. 2020. Nat Commun. 11:99. PubMed Link CWM, et al. 2020. Front Immunol. 11:596772. PubMed Jun JC, et al. 2017. PLoS One. 12:e0180688. PubMed Cané S, et al. 2021. J Immunother Cancer. 9:. PubMed
RRID: AB_2562798 (BioLegend Cat. No. 101913)
Structure: Forms high affinity IL-2R with IL-2Rβ (CD122) and IL-2Rγ (CD132), 55 kD
Distribution: Activated T cells and B cells, thymocyte subset, pre-B cells, T regulatory cells
Function: IL-2 receptor, signal transduction
Ligand/Receptor: IL-2
Cell Type: B cells, T cells, Tregs
Biology Area: Immunology
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors
Antigen References: 1. Taniguchi T, et al. 1993. Cell 73:5. 2. Waldmann TA. 1991. J. Biol. Chem. 266:2681. 3. Read S, et al. 2000. J. Exp. Med. 192:295. 4. Lowenthal JW, et al. 1985. J. Immunol. 135:3988.
Gene ID: 16184
UniProt: View information about CD25 on UniProt.org
Clone: 3C7
Regulatory Status: RUO
Other Names: IL-2Rα, Ly-43, p55, Tac
Isotype: Rat IgG2b, κ
Q: Can I obtain CyTOF data related to your Maxpar® Ready antibody clones?
A: We do not test our antibodies by mass cytometry or on a CyTOF machine in-house. The data displayed on our website is provided by Fluidigm®. Please contact Fluidigm® directly for additional data and further details.

http://techsupport.fluidigm.com/
Q: Can I use Maxpar® Ready format clones for flow cytometry staining?
A: We have not tested the Maxpar® Ready antibodies formulated in solution containing EDTA for flow cytometry staining. While it is likely that this will work in majority of the situations, it is best to use the non-EDTA formulated version of the same clone for flow cytometry testing. The presence of EDTA in some situations might negatively affect staining.
Q: I am having difficulty observing a signal after conjugating a metal tag to your Maxpar® antibody. Please help troubleshoot.
A: We only supply the antibody and not test that in house. Please contact Fluidigm® directly for troubleshooting advice: http://techsupport.fluidigm.com/
Q: Is there a difference between buffer formulations related to Maxpar® Ready and purified format antibodies?
A: The Maxpar® Ready format antibody clones are formulated in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. The regular purified format clones are formulated in solution that does not contain any EDTA. Both formulations are however without any extra carrier proteins.