Biolegend, 101915, PE/Cyanine7 anti-mouse CD25 Antibody, 25μg

CD25 is a 55 kD glycoprotein, also known as the low affinity IL-2Rα, Ly-43, p55, or Tac. It is expressed on activated T and B cells, thymocyte subset, pre-B cells, and T regulatory cells. In association with CD122 (IL-2Rβ) and CD132(common γ chain), CD25 forms the high affinity signaling IL-2 receptor.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: IL-2-dependent BALB/c mouse helper T-cell clone HT-2
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications (for the relevant formats) include: in vitro blocking of IL-2 binding to low- and high-affinity receptors1,2, and immunohistochemical staining of acetone-fixed frozen sections. 3C7 antibody recognizes different epitope of PC61 antibody (Cat. No. 102002). The Ultra-LEAF™Purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 101925 and 101926).
Application References(PubMed link indicates BioLegend citation): Ortega RG, et al. 1984. J. Immunol. 133:1970. (Block) Moreau JL, et al. 1987. Eur. J. Immunol. 17:929. (Block)
Product Citations: Schroeter CB, et al. 2022. J Neuroinflammation. 19:270. PubMed Swan SL, et al. 2023. Front Immunol. 14:1085547. PubMed Lin YN, et al. 2022. Oncoimmunology. 11:2027136. PubMed Hickey A, et al. 2021. Front Microbiol. 12:653587. PubMed Sun CC, et al. 2020. Genome Med. 0.553472222. PubMed Zirngibl F, et al. 2021. J Immunother Cancer. 9:. PubMed MacDonald A, et al. 2021. Front Immunol. 12:755995. PubMed Yang K, et al. 2022. J Clin Invest. 132:. PubMed Lou Y, et al. 2021. Int J Mol Sci. 22:. PubMed Gong N, et al. 2020. Nat Nanotechnol. 1.35625. PubMed Ouyang S, et al. 2019. J Immunol. 202:1441. PubMed Waight JD, et al. 2018. Cancer Cell. 33:1033. PubMed Kim SI, et al. 2020. Molecular Cancer Therapeutics. 20(1):173-182. PubMed Young JS, et al. 2018. Front Immunol. 9:1385. PubMed Wang Y, et al. 2022. Nat Cell Biol. 24:230. PubMed
RRID: AB_2616761 (BioLegend Cat. No. 101915) AB_2616762 (BioLegend Cat. No. 101916)
Structure: Forms high affinity IL-2R with IL-2Rβ (CD122) and IL-2Rγ (CD132), 55 kD
Distribution: Activated T cells and B cells, thymocyte subset, pre-B cells, T regulatory cells
Function: IL-2 receptor, signal transduction
Ligand/Receptor: IL-2
Cell Type: B cells, T cells, Tregs
Biology Area: Immunology
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors
Antigen References: 1. Taniguchi T, et al. 1993. Cell 73:5. 2. Waldmann TA. 1991. J. Biol. Chem. 266:2681. 3. Read S, et al. 2000. J. Exp. Med. 192:295. 4. Lowenthal JW, et al. 1985. J. Immunol. 135:3988.
Gene ID: 16184
UniProt: View information about CD25 on UniProt.org
Clone: 3C7
Regulatory Status: RUO
Other Names: IL-2Rα, Ly-43, p55, Tac
Isotype: Rat IgG2b, κ