Biolegend, 102124, PE/Dazzle™ 594 anti-mouse CD28 Antibody, 100μg

CD28 is a 44 kD glycoprotein, also known as Tp44 or T44. It is a member of the Ig superfamily, expressed on thymocytes, most peripheral T cells, and NK cells. In association with CD80 (B7-1) and CD86 (B7-2), CD28 acts as the second signal for T and NK cell activation and proliferation. The 37.51 antibody has been reported to augment in vitro T cell proliferation and cytokine production, and promote CTL development.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Syrian Hamster
Immunogen: C57BL/6 mouse T-cell lymphoma EL-4
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Dazzle™ 594 under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * PE/Dazzle™ 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro costimulation of T and NK cells1, in vitro blocking of allogeneic mixed leukocyte response and inhibition of MHC-unrestricted CTL cytotoxicity3,4, in vitro induction of thymocyte differentiation2,5-9,11, and immunohistochemical staining of acetone-fixed frozen sections. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) (Cat. No. 102116).
Application References(PubMed link indicates BioLegend citation): Gross JA, et al. 1992. J. Immunol. 149:380. (IP, Costim) Cibotti R, et al. 1997. Immunity 6:245. (Costim) Masten BJ, et al. 1997. Am. J. Respir. Cell Mol. Biol. 16:335. (Block) Nishio M, et al. 1996. J. Immunol. 157:4347. (Block) Zhang N and He Y-W, 2005. J. Exp. Med. 202:395. (Costim) Terrazas LI, et al. 2005. Intl. J. Parasitology. 35:1349. (Costim) Perchonock CE, et al. 2006. Mol Cell Biol. 26(16):6005. (Costim) Wang W, et al. 2007. J. Immunol. 178:4885. (Costim) Pua HH, et al. 2007. J. Exp. Med. 204:25. (Costim) Perchonock CE, et al. 2007. J. Immunol. 179:1768. Barbi J, et al. 2007. Blood 110:2215. Milpied P, et al. 2011. Blood 118:2993. PubMed Cunningham NR, et al. 2011. Int Immunol. 23:693. PubMed Crispin JC, et al. 2012. J. Immunol. 188:3567. PubMed Li CR, et al. 2014. J Immunol. 192:1425. PubMed Blankenhaus B, et al. 2014. PLoS Pathog. 10:1003913. PubMed
Product Citations: Kelsey E Sivick et al. 2018. Cell reports. 25(11):3074-3085 . PubMed
RRID: AB_2629549 (BioLegend Cat. No. 102123) AB_2629550 (BioLegend Cat. No. 102124)
Disclaimer: This product may be used for research purposes only. It is not licensed for resale and may only be used by the buyer. This product may not be used and is not licensed for clinical assays where the results of such assays are provided as a diagnostic service. If a diagnostic or therapeutic use is anticipated then a license must be requested from the University of California. The availability of such diagnostic and therapeutic use license(s) cannot be guaranteed from the University of California.
Structure: Ig superfamily, 44 kD
Distribution: Thymocytes, CD4+, CD8+ peripheral T cells, NK cells
Function: Costimulates T and NK cells
Ligand/Receptor: CD80 (B7-1), CD86 (B7-2)
Cell Type: NK cells, T cells, Thymocytes, Tregs
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Lenschow DJ, et al. 1996. Annu. Rev. Immunol. 14:233. 3. Gross JA, et al. 1992. J. Immunol. 149:380.
Gene ID: 12487
UniProt: View information about CD28 on UniProt.org
Clone: 37.51
Regulatory Status: RUO
Other Names: Tp44, T44
Isotype: Syrian Hamster IgG