Biolegend, 102410, APC anti-mouse CD31 Antibody, 100μg

CD31 is a 130-140 kD glycoprotein, also known as platelet endothelial cell adhesion molecule (PECAM-1) and EndoCAM. It is a member of the Ig superfamily, expressed on endothelial cells, platelets, granulocytes, monocytes/macrophages, dendritic cells, and T and B cell subsets, and is critical for cell-cell interactions. The primary ligands for CD31 have been reported to be CD38 and the vitronectin receptor (α v β 3 integrin, CD51/CD61). Other reported functions of CD31 are neutrophil emigration to sites of inflammation and angiogenesis.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: C3H/HeJ mouse hematopoietic progenitor cell line 3
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Red Laser (633 nm)
Application Notes: Anti-mouse CD31 clones 390 and MEC13.3 bind to their respective non-overlapping epitopes in IgD2 of CD31.8 Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro and in vivo blocking of CD31-mediated cell-cell interactions1-4, and immunohistochemical staining5,6,7 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections. Special Note: This antibody is not recommended for formalin-fixed paraffin-embedded sections. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 102412).
Application References(PubMed link indicates BioLegend citation): Baldwin HS, et al. 1994. Development 120:2539. (IP, Block) DeLisser HM, et al. 1997. Am. J. Pathol. 151:671. (Block) Rosenblum WI, et al. 1996. Stroke 27:709. (Block) Iguchi A, et al. 1997. Cell Struct. Funct. 22:357. (Block) Wyder L, et al. 2000. Cancer Res. 60:4682. (IHC) Wiewrodt R, et al. 2002. Blood 99:912. (IHC) McQualter JL, et al. 2009. Stem Cells. 27:623. (IHC) PubMed Chacko AM, et al. 2012. PLoS One 7:e34958. Greineder CF, et al. 2013. PLoS One. 14:80110. PubMed
Product Citations: Hudson WH, et al. 2020. Immunity. 51(6):1043-1058.e4.. PubMed Chang J, et al. 2023. Research (Wash D C). 6:0120. PubMed Kalgudde Gopal S, et al. 2023. Nat Commun. 14:3020. PubMed Yang D, et al. 2022. Cell. 185:1905. PubMed Ferrao Blanco MN, et al. 2022. Br J Pharmacol. 179:2771. PubMed Kozuki S, et al. 2022. Genes Cells. 27:192. PubMed Lloyd-Lewis B, et al. 2022. Cell Rep. 38:110461. PubMed Tsumita T, et al. 2022. Int J Cancer. 151:944. PubMed Kuo CS, et al. 2022. Elife. 11: . PubMed Gao Q, et al. 2019. J Exp Med. 216:688. PubMed Wang J, et al. 2019. Front Immunol. 9:3157. PubMed Leimkühler NB, et al. 2020. Cell Stem Cell. 28:637. PubMed Cen B, et al. 2021. Oncogene. 40:5984. PubMed Yeo S, et al. 2016. Cancer Res . 76: 3397 - 3410. PubMed Park M, et al. 2021. BMB Rep. 54:470. PubMed Srivastava N, et al. 2020. FASEB J. 34:12805. PubMed Hesse J, et al. 2021. eLife. 10:00. PubMed He W et al. 2018. Immunity. 49(6):1175-1190 . PubMed Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed Pal B, et al. 2017. Nat Commun.. 10.1038/s41467-017-01560-x. PubMed Zhang B, et al. 2021. Nat Biomed Eng. 5:1288. PubMed Quintana E, et al. 2012. Sci Transl Med. 149:159. PubMed Linehan JL et al. 2018. Cell. 172(4):784-796 . PubMed Eskiocak U, et al. 2016. Nat Commun. 7:12336. PubMed Chauveau A, et al. 2020. Immunity. 52:794. PubMed Zeng Z, et al. 2022. Oncogene. :. PubMed Chu X, et al. 2021. Front Cell Dev Biol. 9:671841. PubMed Sarvi S et al. 2018. Cell chemical biology. 25(12):1456-1469 . PubMed Long JT, et al. 2022. Elife. 11:. PubMed Zhang Z, et al. 2021. Exp Dermatol. 30:102. PubMed Yang T, et al. 2022. Antioxidants (Basel). 11:. PubMed Ko HJ, et al. 2020. Front Immunol. 1897:11. PubMed Maroni G, et al. 2019. Int J Mol Sci. 20. PubMed Carmody C et al. 2019. Endocrinology. 160(5):1205-1222 . PubMed Fu NY, et al. 2018. Dev Cell. 47:629. PubMed Yeo SK, et al. 2020. eLife. 9:e58810.. PubMed Wong NR, et al. 2021. Immunity. 54:2072. PubMed Santini MP, et al. 2020. Cell Rep. 30:555. PubMed Wang X, et al. 2018. Nat Commun. 9:551. PubMed Hendee K, et al. 2021. Aging (Albany NY). 13:7781. PubMed Singhal P, et al. 2016. Proc Natl Acad Sci U S A. 113: 122 - 127. PubMed DeVilbiss AW, et al. 2021. eLife. 10:00. PubMed Shen B, et al. 2019. Elife. 8:e42274. PubMed Kalinina A, et al. 2022. PLoS One. 17:e0250752. PubMed Phansalkar R, et al. 2021. Elife. 10:. PubMed Kim KS, et al. 2022. JCI Insight. 7:. PubMed Ouadah Y, et al. 2019. Cell. 179:403. PubMed Nagatake T, et al. 2021. Int Immunol. 33:171. PubMed Pal B, et al. 2021. Breast Cancer Res. 23:69. PubMed Dumm RE, et al. 2020. Cell Reports. 32(9):108103.. PubMed Longchamp A, et al. 2018. Cell. 173:117. PubMed
RRID: AB_312904 (BioLegend Cat. No. 102409) AB_312905 (BioLegend Cat. No. 102410)
Structure: Ig superfamily, 130-140 kD
Distribution: Endothelial cells, platelets, granulocytes, monocytes/macrophages, dendritic cells, T and B cell subsets
Function: Adhesion
Ligand/Receptor: CD38, αV/β3 integrin
Cell Type: B cells, Dendritic cells, Endothelial cells, Granulocytes, Macrophages, Monocytes, Neutrophils, Platelets, T cells
Biology Area: Angiogenesis, Cell Adhesion, Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. DeLisser HM, et al. 1994. Immunol. Today 15:490. 3. Newman PJ, et al. 1990. Science 247:1219.
Gene ID: 18613
UniProt: View information about CD31 on UniProt.org
Clone: 390
Regulatory Status: RUO
Other Names: PECAM-1, EndoCAM
Isotype: Rat IgG2a, κ