Biolegend, 102414, Alexa Fluor® 488 anti-mouse CD31 Antibody, 100μg

CD31 is a 130-140 kD glycoprotein, also known as platelet endothelial cell adhesion molecule (PECAM-1) and EndoCAM. It is a member of the Ig superfamily, expressed on endothelial cells, platelets, granulocytes, monocytes/macrophages, dendritic cells, and T and B cell subsets, and is critical for cell-cell interactions. The primary ligands for CD31 have been reported to be CD38 and the vitronectin receptor (α v β 3 integrin, CD51/CD61). Other reported functions of CD31 are neutrophil emigration to sites of inflammation and angiogenesis.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: C3H/HeJ mouse hematopoietic progenitor cell line 3
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Blue Laser (488 nm)
Application Notes: Anti-mouse CD31 clones 390 and MEC13.3 bind to their respective non-overlapping epitopes in IgD2 of CD31.8 Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro and in vivo blocking of CD31-mediated cell-cell interactions1-4, and immunohistochemical staining5,6,7 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections. Special Note: This antibody is not recommended for formalin-fixed paraffin-embedded sections. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 102412).
Application References(PubMed link indicates BioLegend citation): Baldwin HS, et al. 1994. Development 120:2539. (IP, Block) DeLisser HM, et al. 1997. Am. J. Pathol. 151:671. (Block) Rosenblum WI, et al. 1996. Stroke 27:709. (Block) Iguchi A, et al. 1997. Cell Struct. Funct. 22:357. (Block) Wyder L, et al. 2000. Cancer Res. 60:4682. (IHC) Wiewrodt R, et al. 2002. Blood 99:912. (IHC) McQualter JL, et al. 2009. Stem Cells. 27:623. (IHC) PubMed Chacko AM, et al. 2012. PLoS One 7:e34958. Greineder CF, et al. 2013. PLoS One. 14:80110. PubMed
Product Citations: Pernet E, et al. 2023. Nature. 614:530. PubMed Gomez-Salinero JM, et al. 2022. Cell Stem Cell. 29:593. PubMed Kazakevych J, et al. 2020. Genome Biol. 21:64. PubMed Masschelein E, et al. 2020. Skelet Muscle. 10:21. PubMed Yip HYK, et al. 2021. STAR Protocols. 2(3):100765. PubMed Watanabe S, et al. 2015. J Virol. 89:5847. PubMed Hsieh C, et al. 2014. J Immunol. 193:3693. PubMed Cheng Q, et al. 2020. Nat Nanotechnol. 0.842361111. PubMed Thiriot A et al. 2017. BMC biology. 15(1):45 . PubMed Cohen SB et al. 2018. Cell host & microbe. 24(3):439-446 . PubMed Mizbani A, et al. 2016. Development. 143: 4137 - 4148. PubMed Yip HYK, et al. 2020. Molecular Cell. 80(2):279-295.e8. PubMed Melhem H, et al. 2022. Mucosal Immunol. 15:443. PubMed Kirkby N, et al. 2012. Proc Natl Acad Sci U S A. 109:17597. PubMed Fan Z, et al. 2021. Cell Metabolism. 33:1793. PubMed Amezcua Vesely MC, et al. 2020. Cell. 178(5):1176-1188.e15.. PubMed Kazakevych J, et al. 2019. Sci Rep. 9:10410. PubMed Losenkova K, et al. 2020. J Cell Sci. 133:00:00. PubMed Chojnacki A, et al. 2019. Commun Biol. 2:181. PubMed Namachivayam K, et al. 2020. Proc Natl Acad Sci U S A. 117:10958. PubMed Barry DM, et al. 2019. Nat Commun. 4.378472222. PubMed Lupan AM, et al. 2021. Aging (Albany NY). 13:15875. PubMed
RRID: AB_493409 (BioLegend Cat. No. 102413) AB_493408 (BioLegend Cat. No. 102414)
Structure: Ig superfamily, 130-140 kD
Distribution: Endothelial cells, platelets, granulocytes, monocytes/macrophages, dendritic cells, T and B cell subsets
Function: Adhesion
Ligand/Receptor: CD38, αV/β3 integrin
Cell Type: B cells, Dendritic cells, Endothelial cells, Granulocytes, Macrophages, Monocytes, Neutrophils, Platelets, T cells
Biology Area: Angiogenesis, Cell Adhesion, Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. DeLisser HM, et al. 1994. Immunol. Today 15:490. 3. Newman PJ, et al. 1990. Science 247:1219.
Gene ID: 18613
UniProt: View information about CD31 on UniProt.org
Clone: 390
Regulatory Status: RUO
Other Names: PECAM-1, EndoCAM
Isotype: Rat IgG2a, κ