Biolegend, 102720, Pacific Blue™ anti-mouse CD38 Antibody, 100μg

CD38 is a 45 kD type II transmembrane glycoprotein also known as T10. It is an ADP-ribosyl hydrolase expressed at variable levels on hematopoietic cells and in some non-hematopoietic tissues (such as brain, muscle, and kidney). In humans, it is expressed at high levels on plasma cells and activated T and B cells, natural killer (NK) lymphocytes, myeloblasts, and erythroblasts. By functioning as both a cyclase and a hydrolase, CD38 mediates lymphocyte activation, adhesion, and the metabolism of cADPR and NAADP. CD31 is the ligand of CD38.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse bone marrow pre-B cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Violet Laser (405 nm)
Product Citations: Chen Q, et al. 2022. Cell Rep. 39:110990. PubMed Schaefer-Babajew D, et al. 2023. Nature. 613:735. PubMed Hägglöf T, et al. 2023. Cell. 186:147. PubMed Kodali S, et al. 2022. J Immunol. 208:1085. PubMed Pack AD, et al. 2021. Cell Reports. 36:109586. PubMed Onodera T, et al. 2019. J Immunol. 203:3282. PubMed Chen M, et al. 2016. J Immunol . 194: 2607-15. PubMed Viant C, et al. 2021. J Exp Med. 218:. PubMed Wang JC, et al. 2022. Elife. 11:. PubMed Ma K, et al. 2022. iScience. 25:104347. PubMed Yang P et al. 2019. Cell Syst. 8(5):427-445 . PubMed Ghosh D, et al. 2016. J Immunol. 197: 1788 - 1800. PubMed Adachi Y, et al. 2019. Nat Commun. 10:3883. PubMed Lutz J, et al. 2015. Nat Commun. 6: 8575. PubMed Viant C, et al. 2020. Cell. 183:1298. PubMed Adachi Y, et al. 2015. J Exp Med. 212: 1709-1723. PubMed Viant C, et al. 2021. STAR Protocols. 2(2):100389. PubMed Jang A, et al. 2021. Front Immunol. 12:701066. PubMed
RRID: AB_10613289 (BioLegend Cat. No. 102719) AB_10613468 (BioLegend Cat. No. 102720)
Structure: ADP-ribosyl hydrolase, 42 kD
Distribution: B cells, NK cells, T cell subset, brain, muscle, kidney
Function: Regulation of cellular activation/proliferation, adhesion, metabolism of cADPR and NAADP
Ligand/Receptor: CD31, hyaluronic acid
Antigen References: 1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Shubinsky G, et al. 1997. Immunity 7:315. 3. Cesano A, et al. 1998. J. Immunol. 160:1106. 4. Cockayne DA, et al. 1998. Blood 92:1324.
Gene ID: 12494
UniProt: View information about CD38 on UniProt.org
Clone: 90
Regulatory Status: RUO
Other Names: T10
Isotype: Rat IgG2a, κ