Biolegend, 102722, PerCP/Cyanine5.5 anti-mouse CD38 Antibody, 100μg

CD38 is a 45 kD type II transmembrane glycoprotein also known as T10. It is an ADP-ribosyl hydrolase expressed at variable levels on hematopoietic cells and in some non-hematopoietic tissues (such as brain, muscle, and kidney). In humans, it is expressed at high levels on plasma cells and activated T and B cells, natural killer (NK) lymphocytes, myeloblasts, and erythroblasts. By functioning as both a cyclase and a hydrolase, CD38 mediates lymphocyte activation, adhesion, and the metabolism of cADPR and NAADP. CD31 is the ligand of CD38.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse bone marrow pre-B cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser: Blue Laser (488 nm)
Product Citations: Apte SH, et al. 2020. Clin Transl Immunology. 9:e1209. PubMed Hu Q, et al. 2022. Cell Rep. 40:111035. PubMed Kodali S, et al. 2022. J Immunol. 208:1085. PubMed Lee JL, et al. 2022. Aging Cell. 21:e13692. PubMed Fallet B, et al. 2020. Cell Rep. 30:1013. PubMed Krueger CC, et al. 2019. Front Immunol. 10:1831. PubMed Kräutler NJ, et al. 2020. Cell Reports. 30(4):997-1012.e6.. PubMed Muri J, et al. 2020. Cell Reports. 29(9):2731-2744.e4.. PubMed Chang X, et al. 2022. Front Immunol. 12:827256. PubMed
RRID: AB_2563332 (BioLegend Cat. No. 102721) AB_2563333 (BioLegend Cat. No. 102722)
Structure: ADP-ribosyl hydrolase, 42 kD
Distribution: B cells, NK cells, T cell subset, brain, muscle, kidney
Function: Regulation of cellular activation/proliferation, adhesion, metabolism of cADPR and NAADP
Ligand/Receptor: CD31, hyaluronic acid
Antigen References: 1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Shubinsky G, et al. 1997. Immunity 7:315. 3. Cesano A, et al. 1998. J. Immunol. 160:1106. 4. Cockayne DA, et al. 1998. Blood 92:1324.
Gene ID: 12494
UniProt: View information about CD38 on UniProt.org
Clone: 90
Regulatory Status: RUO
Other Names: T10
Isotype: Rat IgG2a, κ
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.