Biolegend, 103105, PE anti-mouse CD45 Antibody, 50μg

CD45 is a 180-240 kD glycoprotein also known as the leukocyte common antigen (LCA), T200, or Ly-5. It is a member of the protein tyrosine phosphatase (PTP) family, expressed on all hematopoietic cells except mature erythrocytes and platelets. There are different isoforms of CD45 that arise from variable splicing of exons 4, 5, and 6, which encode A, B, and C determinants, respectively. CD45 plays a key role in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation state of the cell as well as cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.
50μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse thymus or spleen
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Clone 30-F11 reacts with all isoforms and both CD45.1 and CD45.2 alloantigens of CD45. Additional reported applications (for relevant formats) include: immunoprecipitation3, complement-dependent cytotoxicity1,5, immunohistochemistry (acetone-fixed frozen sections, zinc-fixed paraffin-embedded sections and formalin-fixed paraffin-embedded sections)4,6, Western blotting7, and spatial biology (IBEX)10,11. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 103163 and 103164).
Application References(PubMed link indicates BioLegend citation): Podd BS, et al. 2006. J. Immunol. 176:6532. (FC, CMCD) PubMed Haynes NM, et al. 2007. J. Immunol. 179:5099. (FC) Ledbetter JA, et al. 1979. Immunol. Rev. 47:63. (IP) Simon DI, et al. 2000. J. Clin. Invest. 105:293. (IHC) Seaman WE. 1983. J. Immunol. 130:1713. (CMCD) Cornet A, et al. 2001. P. Natl. Acad. Sci. USA 98:13306. (IHC) Tsuboi S and Fukuda M. 1998. J. Biol. Chem. 273:30680. (WB) PubMed Liu F, et al. 2012. Blood. 119:3295. PubMed Pelletier AN, et al. 2012. J. Immunol. 188:5561. PubMed Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations: Kretschmer I, et al. 2016. J Biol Chem. 291: 4091 - 4106. PubMed Moreno-García Á, et al. 2020. Biomolecules. 10:00. PubMed Liu K, et al. 2021. Cell Metabolism. 33(3):666-675.e4. PubMed Vercauteren Drubbel A, et al. 2021. Cell Stem Cell. . PubMed Frieler RA, et al. 2017. Exp Neurol. 298:104. PubMed Djoki&cacute; V, et al. 2019. Front Immunol. 9:2891. PubMed Hepler C et al. 2017. eLife. 6 pii: e27669. PubMed Chan LC, et al. 2019. J Clin Invest. 129:3324. PubMed Khorooshi R, et al. 2020. Acta Neuropathol Commun. 8:02. PubMed Kimura S, et al. 2020. Nat Commun. 0.620833333. PubMed Masuda H, et al. 2022. MAbs. 14:2122275. PubMed Shi H, et al. 2022. Nutrients. 14:. PubMed Peng W, et al. 2022. Cell Mol Life Sci. 79:602. PubMed Elbasiony E, et al. 2023. NPJ Aging. 9:2. PubMed Pioli KT, et al. 2023. iScience. 26:106223. PubMed Dang YZ, et al. 2023. Neoplasma. 70:123. PubMed Palakurthi B, et al. 2023. Nat Commun. 14:2109. PubMed Shen Z, et al. 2023. iScience. 26:106572. PubMed Abdelwahab T, et al. 2023. iScience. 26:106698. PubMed Linden J, et al. 2020. J Interferon Cytokine Res. 40:292. PubMed Ramos SA, et al. 2022. J Allergy Clin Immunol. 149:767. PubMed Friesen M, et al. 2022. Brain Pathol. 32:e13032. PubMed Kang H, et al. 2022. Adv Mater. 34:e2106500. PubMed Brandt KJ, et al. 2022. Int J Mol Sci. 23: . PubMed Cha JH, et al. 2022. J Biol Chem. 298:101817. PubMed Cox MJ, et al. 2022. Leukemia. 36:1635. PubMed Yu C, et al. 2022. Invest Ophthalmol Vis Sci. 63:1. PubMed Grégoire C, et al. 2022. Immunity. 55:1216. PubMed Liu X, et al. 2022. Chin Med J (Engl). 135:1821. PubMed Zhang X, et al. 2022. EBioMedicine. 84:104275. PubMed Dedousis N, et al. 2022. J Lipid Res. 63:100284. PubMed Yang J, et al. 2022. Mol Ther Nucleic Acids. 30:184. PubMed Hirano K, et al. 2023. Life Sci Alliance. 6: . PubMed Ding L, et al. 2023. J Clin Invest. 133: . PubMed Al-Saafeen BH, et al. 2023. Front Immunol. 13:1017780. PubMed Montilla A, et al. 2023. Cell Death Dis. 14:16. PubMed Smith LA, et al. 2023. Front Oncol. 12:1031174. PubMed Gonçalves R, et al. 2023. iScience. 26:105972. PubMed Wang Y, et al. 2023. FASEB J. 37:e22776. PubMed Tanaka T, et al. 2023. Nat Immunol. 24:439. PubMed Wang R, et al. 2023. iScience. 26:105954. PubMed Vuerich R, et al. 2023. NPJ Regen Med. 8:8. PubMed Tan Y, et al. 2023. NPJ Regen Med. 8:7. PubMed Li Z, et al. 2022. Nat Commun. 13:1845. PubMed Sun D, et al. 2021. Cell Stem Cell. . PubMed Furuta Y, et al. 2017. PLoS One. 12(2):e0172509. PubMed Weng L, et al. 2016. Sci Rep. 6:38632. PubMed Hagan N, et al. 2020. Cell Death Dis. 1.086111111. PubMed Giorgetti E, et al. 2020. Cell Reports. 29(6):1539-1554.e7.. PubMed Wu N, et al. 2020. Cell Rep. 30:1129. PubMed Abels ER et al. 2019. Cell Rep. 28(12):3105-3119 . PubMed Xin G, et al. 2021. Cancer Immunol Res. 9:454. PubMed Qi Z, et al. 2022. Nat Commun. 13:182. PubMed d'Errico P, et al. 2021. Nat Neurosci. Online ahead of print. PubMed Afolabi LO, et al. 2021. Front Immunol. 12:701671. PubMed Maimets M, et al. 2016. Stem Cell Reports. 6:150-162. PubMed Karlen SJ, et al. 2018. J Neuroinflammation. 15:344. PubMed Cieslik KA, et al. 2018. J Gerontol A Biol Sci Med Sci. 73:1167. PubMed Kobayashi Y, et al. 2018. PLoS One. 13:e0195008. PubMed Riley JS, et al. 2020. Blood Adv. 4:1102. PubMed Chen J, et al. 2021. Sci Adv. 7:. PubMed Rodriguez-García A, et al. 2021. Nat Commun. 12:877. PubMed Jenull S, et al. 2021. Cell Reports. 36(3):109406. PubMed Fan C, et al. 2020. Stem Cell Res Ther. 0.765277778. PubMed Wei H, et al. 2020. Cell Death Dis. 11:290. PubMed Ogawa M, et al. 2019. J Biol Methods. 5:e102. PubMed Shmeleva EV, et al. 2019. Front Immunol. 10:1780. PubMed Shu T, et al. 2022. Eur Respir J. Online ahead of print. PubMed Tilstam PV, et al. 2021. J Clin Invest. 131:. PubMed Catrina AM, et al. 2021. Rom J Morphol Embryol. 62:109. PubMed Aoki H, et al. 2009. Development. 136:2511. PubMed Guha S, et al. 2014. Brain Behav Immun. 11:123. PubMed Verma M, et al. 2018. Brain Behav Immun. 69:223. PubMed Duarte N, et al. 2018. Hepatol Commun. 0.833333333. PubMed Rafiq S, et al. 2018. Nat Biotechnol. 36:847. PubMed Joseph R, et al. 2021. Br J Cancer. 125:176. PubMed Wang Y, et al. 2021. Nat Commun. 12:2343. PubMed Helm M, et al. 2022. Life (Basel). 12:. PubMed Glaubitz J, et al. 2022. Nat Commun. 13:4502. PubMed Sun Q, et al. 2022. Cell Death Dis. 13:724. PubMed Galle E, et al. 2022. Genome Biol. 23:207. PubMed Endo Y, et al. 2020. FASEB J. 34:16086. PubMed An YA et al. 2017. eLife. 6 pii: e24071. PubMed Meixiong J et al. 2019. Immunity. 50(5):1163-1171 . PubMed Cao W, et al. 2017. Immunity. 47:1182. PubMed Hsu JM, et al. 2018. Nat Commun. 9:1908. PubMed Serrano Martinez P, et al. 2022. iScience. 25:105047. PubMed Ogawa-Wong A, et al. 2022. Metabolites. 12:. PubMed Hongu T, et al. 2022. Nat Cancer. 3:486. PubMed Vijay R, et al. 2015. J Exp Med. 212: 1851 - 1868. PubMed Dumas AA, et al. 2020. EMBO J. 39:e103790. PubMed Fontana D, et al. 2020. Hemasphere. e497:4. PubMed He W et al. 2018. Immunity. 49(6):1175-1190 . PubMed Green DP, et al. 2019. Neuron. 101:412. PubMed Hillel–Karniel C, et al. 2020. Cell Reports. 30(3):807-819.e4.. PubMed An J, et al. 2022. iScience. 25:103570. PubMed Shen Y, et al. 2022. CNS Neurosci Ther. 28:46. PubMed Ye Z, et al. 2019. Mediators Inflamm. 2019:8709583. PubMed Miska J et al. 2019. Cell reports. 27(1):226-237 . PubMed Wang GZ, et al. 2019. Nat Commun. 10:1125. PubMed Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed Mencarelli A, et al. 2016. Sci Rep. 6:30802. PubMed Yang Z, et al. 2020. Sci Rep. 17757:10. PubMed Socodato R, et al. 2020. Sci Signal. 13: . PubMed Sardella C, et al. 2018. J Invest Dermatol. 138:500. PubMed Yee PP, et al. 2020. Nat Commun. 4.225. PubMed Oliver J, et al. 2009. J Am Soc Nephrol. 2.440972222. PubMed Marinescu CI, et al. 2021. Stem Cell Res Ther. 12:319. PubMed Chang CS, et al. 2022. iScience. 25:103738. PubMed Sinha VC, et al. 2021. Nat Commun. 12:5024. PubMed Formaglio P, et al. 2021. Immunity. 54:2724. PubMed Hu J, et al. 2022. J Neuroinflammation. 19:71. PubMed Slezak AJ, et al. 2022. ACS Cent Sci. 8:1435. PubMed Yu Y, et al. 2022. Nat Commun. 13:6857. PubMed Khatib–Massalha E, et al. 2020. Nat Commun. 2.921527778. PubMed Chauveau A, et al. 2020. Immunity. 52:794. PubMed Qi S et al. 2016. eLife. 5 pii: e14756. PubMed Bauer T, et al. 2021. Life (Basel). 11:. PubMed Ye P, et al. 2022. Front Cardiovasc Med. 8:810477. PubMed Ebrahimi-Nik H, et al. 2021. Nat Commun. 12:6423. PubMed Olson M, et al. 2014. J Immunol. 193:5420. PubMed Barker KA, et al. 2021. Journal of Clinical Investigation. 131(11):. PubMed Fang Y, et al. 2019. Aging (Albany NY). 11:4910. PubMed Wilson DH, et al. 2020. Nat Commun. 0.767361111. PubMed Ito T, et al. 2021. Commun Biol. 4:694. PubMed Liu X, et al. 2020. Cell Host Microbe. 28(5):683-698.e6. PubMed Walsh SM, et al. 2021. eLife. 10:00. PubMed Madenspacher JH, et al. 2020. JCI Insight. 5:00. PubMed Simula L et al. 2018. Cell reports. 25(11):3059-3073 . PubMed Chen Z, et al. 2019. J Exp Med. 216:152. PubMed Wang QA et al. 2018. Cell metabolism. 28(2):282-288 . PubMed Khare P, et al. 2017. J Autoimmun. 10.1016/j.jaut.2017.09.002. PubMed Feng J,et al. 2017. Nat Commun. . 10.1038/s41467-017-01056-8. PubMed Douam F, et al. 2017. Mbio. 10.1128/mBio.00819-17. PubMed Sun H, et al. 2022. Front Immunol. 13:818017. PubMed Li Y, Kaneda T 2016. Sci Rep. 6: 25077. PubMed Wijewarnasuriya D, et al. 2020. Cancer Immunol Res. 0.841666667. PubMed Bota-Rabassedas N, et al. 2021. Cell Reports. 35(3):109009. PubMed Meng X, et al. 2022. Proc Natl Acad Sci U S A. 119:. PubMed Hu M, et al. 2020. Cancer Immunol Res. 8:1150. PubMed Kitajima Y, et al. 2022. Cells. 11:. PubMed Dulken BW, et al. 2019. Nature. 571:205. PubMed Kamata T, et al. 2020. Cell Reports. 31(12):107802. PubMed Li Z, et al. 2022. Nat Commun. 13:6321. PubMed McCallion A, et al. 2022. Front Immunol. 13:961599. PubMed Matsumoto A, et al. 2021. iScience. 24:102839. PubMed Lee-Chang C, et al. 2021. J Exp Med. 218:. PubMed Wang W, et al. 2021. J Am Heart Assoc. 10:e019142. PubMed Senthil K, et al. 2021. Invest Ophthalmol Vis Sci. 62:38. PubMed Wang T et al. 2018. Immunity. 49(3):504-514 . PubMed Dhar P, et al. 2021. Commun Biol. 4:905. PubMed Dholakia J, et al. 2022. Gynecol Oncol. 164:170. PubMed Wang Y, et al. 2022. Front Immunol. 13:847581. PubMed Kronborg Hansen AK, et al. 2022. Int J Mol Sci. 23:. PubMed Zhang Y, et al. 2021. Commun Biol. 344:4. PubMed Liao Y, et al. 2020. Theranostics. 7.113888889. PubMed Watson N, et al. 2015. J Immunol. 194:2796. PubMed Liu B, et al. 2013. Development. 140:780. PubMed Bao Z, et al. 2020. Ann Transplant. 25:e921287. PubMed Montel‐Hagen A et al. 2019. Cell stem cell. 24(3):376-389 . PubMed Zhao N, et al. 2018. J Clin Invest. 26:84. PubMed Liu X, et al. 2021. Int J Med Sci. 18:3125. PubMed Song E, et al. 2020. Nature. 577:689. PubMed Canedo T, et al. 2021. Neuropsychopharmacology. 46:2358. PubMed Ma L, et al. 2022. Sci Adv. 8:eabm3470. PubMed Milon B, et al. 2021. Cell Rep. 36:109758. PubMed Dieu RS, et al. 2021. Front Neurosci. 15:685645. PubMed Singhal P, et al. 2016. Proc Natl Acad Sci U S A. 113: 122 - 127. PubMed Korin B, et al. 2020. Sleep. :43. PubMed An X, et al. 2020. Thyroid. . PubMed Xu L, et al. 2019. Haematologica. 10.3324/haematol.2018.207258. PubMed Genoud V, et al. 2018. Oncoimmunology. 7:e1501137. PubMed Wang X, et al. 2021. Inflammation. 44:1815. PubMed Sanz-Ros J, et al. 2022. Sci Adv. 8:eabq2226. PubMed Srivastava S, et al. 2020. Cancer Cell. 39(2):193-208.e10. PubMed Bollinger JL, et al. 2020. Neuropsychopharmacology. 45:1766. PubMed Yue F, et al. 2022. Cell Rep. 38:110267. PubMed Andreou T, et al. 2021. J Immunother Cancer. 9:. PubMed Nakao M, et al. 2021. Sci Rep. 11:18384. PubMed Kojima T, et al. 2016. Sci Rep. 6:36457. PubMed Gardenier J, et al. 2017. Nat Commun. 8:14345. PubMed Singh K, et al. 2021. Front Immunol. 640837:12. PubMed Wen G, et al. 2018. J Am Heart Assoc. 7:e008187. PubMed Stone S et al. 2018. Glia. 66(7):1331-1345 . PubMed Orhon I, et al. 2021. Autophagy. :1. PubMed Szõke D, et al. 2021. Nat Commun. 12:3460. PubMed Lau A, et al. 2022. Sci Adv. 8:eabm0142. PubMed Hung G, et al. 2022. NPJ Aging. 8:10. PubMed Gawish R, et al. 2022. Elife. 11:. PubMed Li J, et al. 2021. eLife. 10:00. PubMed Simula L, et al. 2020. Cell Death Differ. 27:2749. PubMed Engelmann R, et al. 2017. J Negat Results Biomed. 16:05. PubMed Tong Y, et al. 2018. EBioMedicine. 39:132. PubMed Lehmann ML, et al. 2018. Sci Rep. 8:11240. PubMed Lehmann M, et al. 2020. Cell Signal. 70:109588. PubMed Cugurra A, et al. 2021. Science. 373:. PubMed Davis FM, et al. 2021. J Exp Med. 218:. PubMed Lupan AM, et al. 2021. Aging (Albany NY). 13:15875. PubMed
RRID: AB_312970 (BioLegend Cat. No. 103105) AB_312971 (BioLegend Cat. No. 103106)
Structure: Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution: All hematopoietic cells except mature erythrocytes and platelets
Function: Phosphatase, T and B cell activation
Ligand/Receptor: Galectin-1, CD2, CD3, CD4, TCR, CD22, Thy-1
Cell Type: B cells, Dendritic cells, Mesenchymal Stem Cells, Tregs
Biology Area: Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family: CD Molecules
Antigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85. 3. Kishihara K, et al. 1993. Cell 74:143. 4. Pulido R, et al. 1988. J. Immunol. 140:3851.
Gene ID: 19264
UniProt: View information about CD45 on UniProt.org
Clone: 30-F11
Regulatory Status: RUO
Other Names: T200, Ly-5, LCA
Isotype: Rat IgG2b, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.