Biolegend, 103154, APC/Fire™ 750 anti-mouse CD45 Antibody, 100μg

CD45 is a 180-240 kD glycoprotein also known as the leukocyte common antigen (LCA), T200, or Ly-5. It is a member of the protein tyrosine phosphatase (PTP) family, expressed on all hematopoietic cells except mature erythrocytes and platelets. There are different isoforms of CD45 that arise from variable splicing of exons 4, 5, and 6, which encode A, B, and C determinants, respectively. CD45 plays a key role in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation state of the cell as well as cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse thymus or spleen
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 750 under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.* APC/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 774 nm.
Excitation Laser: Red Laser (633 nm)
Application Notes: Clone 30-F11 reacts with all isoforms and both CD45.1 and CD45.2 alloantigens of CD45. Additional reported applications (for relevant formats) include: immunoprecipitation3, complement-dependent cytotoxicity1,5, immunohistochemistry (acetone-fixed frozen sections, zinc-fixed paraffin-embedded sections and formalin-fixed paraffin-embedded sections)4,6, Western blotting7, and spatial biology (IBEX)10,11. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 103163 and 103164).
Application References(PubMed link indicates BioLegend citation): Podd BS, et al. 2006. J. Immunol. 176:6532. (FC, CMCD) PubMed Haynes NM, et al. 2007. J. Immunol. 179:5099. (FC) Ledbetter JA, et al. 1979. Immunol. Rev. 47:63. (IP) Simon DI, et al. 2000. J. Clin. Invest. 105:293. (IHC) Seaman WE. 1983. J. Immunol. 130:1713. (CMCD) Cornet A, et al. 2001. P. Natl. Acad. Sci. USA 98:13306. (IHC) Tsuboi S and Fukuda M. 1998. J. Biol. Chem. 273:30680. (WB) PubMed Liu F, et al. 2012. Blood. 119:3295. PubMed Pelletier AN, et al. 2012. J. Immunol. 188:5561. PubMed Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations: Sagnella SM, et al. 2020. Cancer Cell. 37(3):354-370. PubMed Zhang Z, et al. 2022. Mol Nutr Food Res. 66:e2200300. PubMed Zhao Q, et al. 2023. Nutrients. 15: . PubMed Marritt KL, et al. 2023. Front Immunol. 13:1087991. PubMed Wang C, et al. 2023. Exp Mol Med. 55:347. PubMed Jacoby E, et al. 2021. NPJ Regen Med. 6:58. PubMed Bottek J, et al. 2020. Mucosal Immunol. 1.029166667. PubMed Zheng W, et al. 2021. Wellcome Open Research. 6:52. PubMed Israelow B, et al. 2021. Sci Immunol. 6:eabl4509. PubMed Park JJ, et al. 2021. Nat Commun. 12:1222. PubMed Almutairi F, et al. 2021. Front Immunol. 12:772288. PubMed Schaffert A, et al. 2020. Front Immunol. 10:3024. PubMed Fulham MA, et al. 2019. Am J Physiol Cell Physiol. 317:C687. PubMed Sharba S, et al. 2019. Virulence. 10:97. PubMed Hildebrand KM, et al. 2021. PLoS One. 16:e0253864. PubMed Parker KR, et al. 2020. Cell. 183(1):126-142.e17. PubMed Kennedy EM, et al. 2022. Nat Commun. 13:5907. PubMed Levesque S, et al. 2019. Oncoimmunology. 8:e1657375. PubMed Puigdelloses M, et al. 2021. J Immunother Cancer. 9:. PubMed Takahashi F, et al. 2022. iScience. 25:104278. PubMed Lai SM et al. 2018. Cell reports. 25(11):3099-3109 . PubMed Fan Z, et al. 2021. Cell Metabolism. 33:1793. PubMed Subramaniam N, et al. 2021. Blood Adv. 5:1259. PubMed Werner A, et al. 2021. iScience. 24:103076. PubMed Tran S, et al. 2020. Immunity. 53(3):627-640.e5. PubMed Li Z et al. 2018. Immunity. 49(4):640-653 . PubMed Galeano Nio JL, et al. 2020. eLife. 9:00. PubMed
RRID: AB_2572115 (BioLegend Cat. No. 103153) AB_2572116 (BioLegend Cat. No. 103154)
Structure: Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution: All hematopoietic cells except mature erythrocytes and platelets
Function: Phosphatase, T and B cell activation
Ligand/Receptor: Galectin-1, CD2, CD3, CD4, TCR, CD22, Thy-1
Cell Type: B cells, Dendritic cells, Mesenchymal Stem Cells, Tregs
Biology Area: Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family: CD Molecules
Antigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85. 3. Kishihara K, et al. 1993. Cell 74:143. 4. Pulido R, et al. 1988. J. Immunol. 140:3851.
Gene ID: 19264
UniProt: View information about CD45 on UniProt.org
Clone: 30-F11
Regulatory Status: RUO
Other Names: T200, Ly-5, LCA
Isotype: Rat IgG2b, κ