Biolegend, 103316, Pacific Blue™ anti-mouse CD45RB Antibody, 100μg

CD45RB is an isoform of CD45 with exon 5 splicing (encodes B determinant). It is a 220 kD glycoprotein expressed on peripheral B cells, naïve T cells, thymocytes, weakly on macrophages, and dendritic cells. It plays a critical role in TCR and BCR signaling. As T cells become activated and progress from naïve to memory cells, CD45RB expression is downregulated. Additionally, functionally distinct CD4 + T cell subsets, which secrete differing cytokine profiles, can be separated by CD45RB intensity. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4 and Thy-1.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Cloned mouse Th2 cell lines
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is highly recommended that the reagent be titrated for optimal performance for each application. * Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Violet Laser (405 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections.
Application References(PubMed link indicates BioLegend citation): Bottomly K, et al. 1989. Eur. J. Immunol. 19:617. Norian LA and Allen PM. 2004. J. Immunol. 173:835.
Product Citations: Alam MM, et al. 2022. Cell Rep. 110090:38. PubMed Krummey SM, et al. 2020. Cell Reports. 30(5):1282-1291.e5.. PubMed Wilfahrt D, et al. 2021. Elife. 10:. PubMed
RRID: AB_2174406 (BioLegend Cat. No. 103315) AB_2174405 (BioLegend Cat. No. 103316)
Structure: Phosphatase receptor (CD45 gene exon 5), 220 kD
Distribution: Peripheral B cells, naïve T cells, most thymocytes, low density on macrophages and dendritic cells
Function: Phosphatase, T cell development and activation
Ligand/Receptor: Galectin-1, CD2, CD3, CD4, Thy1
Cell Type: B cells, Dendritic cells, Macrophages, T cells, Thymocytes, Tregs
Biology Area: Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
Molecular Family: CD Molecules
Antigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85. 3. Kishihara K, et al. 1993. Cell 74:143. 4. Pulido R, et al. 1988. J. Immunol. 140:3851.
Gene ID: 19264
UniProt: View information about CD45RB on UniProt.org
Clone: C363-16A
Regulatory Status: RUO
Other Names: CD45RB
Isotype: Rat IgG2a, κ