Biolegend, 103801, Purified anti-mouse CD49e Antibody, 50μg

CD49e is a 135 kD protein, also known as α 5 integrin or VLA-5 α chain. It is a member of the integrin family, expressed on thymocytes, splenic B cells, activated T cells, and mast cells. CD49e associates with CD29 (integrin β 1 chain) to form the fibronectin receptor (VLA-5). CD49e plays a critical role in both adhesion and T cell costimulation. The primary ligand for CD49e/CD29 (VLA-5) is fibronectin. The 5H10-27(MFR5) antibody has been shown to block CD49e mediated interactions and promote the in vitro stimulation of CD8 + T cells.
50μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: (C57BL/6 x A/J)F1 mouse mast cell line
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality testedIHC-F - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining6,7 of acetone-fixed frozen sections, blocking of cell-cell adhesion1-5, inhibition of TNF-ß1 costimulated T cell proliferation3, and costimulation of T cell proliferation by cross-linked 5H10-27 antibody3. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 103817 and 103818).
Application References(PubMed link indicates BioLegend citation): Kinashi T, et al. 1994. Blood Cells 20:25. (Block) Halvorson MJ, et al. 1995. J. Immunol. 155:4567. (Block) Rich S, et al. 1996. J. Immunol. 157:2916. (Block Costim)) Uhlenkott CE, et al. 1996. Clin. Exp. Metastasis 14:125. (Block) Schultz JF, et al. 1995. J. Biol. Chem. 270:11522. (Block) Yang JT, et al. 1996. Mol. Biol. Cell. 7:1737. (IHC) Cuklerman E, et al. 2001. Science 294:1708. (IHC) Jia W, et al. 2005. Blood 106:3854. (FC)
Product Citations: Jia W, et al. 2005. Blood. 106:3854. PubMed Hutton C, et al. 2021. Cancer Cell. 39:1227. PubMed Kimura R, et al. 2012. Clin Cancer Res. 1.332638889. PubMed Cegarra C, et al. 2022. PLoS One. 17:e0274667. PubMed
RRID: AB_313050 (BioLegend Cat. No. 103801)
Structure: Integrin family, associates with integrin β1, 135 kD
Distribution: Thymocytes, activated T cells, mast cells
Function: Adhesion, costimulation
Ligand/Receptor: Fibronectin
Cell Type: Mast cells, Mesenchymal Stem Cells, T cells, Thymocytes, Tregs
Biology Area: Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Stem Cells
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Kinashi T, et al. 1994 Blood Cells 20:25. 3. Hemler ME. 1990. Annu. Rev. Immunol. 8:365.
Gene ID: 16402
UniProt: View information about CD49e on UniProt.org
Clone: 5H10-27(MFR5)
Regulatory Status: RUO
Other Names: α5 integrin, VLA-5 α chain, Integrin α5, Fnra, Itga5
Isotype: Rat IgG2a, κ