Biolegend, 104314, Alexa Fluor® 647 anti-mouse/rat CD61 Antibody, 100μg

CD61 is a 110 kD integrin β chain also known as β 3 integrin or gpIIIa. It associates with the integrin α v chain (CD51) to form the vitronectin receptor. In addition, CD61 can associate with the integrin α IIb chain (CD41) to form the gpIIb/IIIa complex. CD61 is expressed on platelets, megakaryocytes, endothelium, smooth muscle, a subset of B cells, myeloid cells, osteoclasts, and mast cells. CD61, in conjunction with CD41 or CD51, mediates adhesion to fibronectin, fibrinogen, vitronectin, thrombospondin, and von Willebrand factor. Leukocyte-endothelial adhesion is mediated by the binding of α v /β 3 integrin or vitronectin receptor to CD31 (PECAM-1).
100μg
Verified Reactivity: Mouse, Rat
Antibody Type: Monoclonal
Host Species: Armenian Hamster
Immunogen: Vitronectin receptor protein from the mouse T-cell hybridoma 2B4.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Application Notes: The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 104325 and 104326).
Application References(PubMed link indicates BioLegend citation): Kieffer N, et al. 1990. Annu. Rev. Cell Biol. 6:329. (Block) Piali L, et al. 1995. J. Cell Biol. 130:451. (Block) Ashkar S, et al. 2000. Science 287:860. (Block) Schultz JF, et al. 1995. J. Biol. Chem. 270:11522. (Block) Moulder K, et al. 1991. J. Exp. Med. 173:343. (Activ) Carlson TR, et al. 2008.135:2193. PubMed Yamaji D, et al. 2009. Genes Dev. 23:2382. PubMed
Product Citations: Yu L, et al. 2020. Cell Stem Cell. 28(3):550-567.e12. PubMed Asselin-Labat M, et al. 2011. Mol Cell Biol. 31:4609. PubMed Elsarraj H, et al. 2013. J Cell Sci. 126:2446. PubMed Lopez DA, et al. 2022. Cell Rep. 41:111677. PubMed Baasch S, et al. 2021. Cell. . PubMed Brait VH, et al. 2019. Circ Res. 124:279. PubMed Gaylo‐Moynihan A et al. 2019. Immunity. 51(2):298-309 . PubMed Laidlaw T, et al. 2012. Blood. 119:3790. PubMed Feng Q et al. 2018. The Journal of physiology. 597(3):781-798 . PubMed
RRID: AB_2249337 (BioLegend Cat. No. 104313) AB_2234024 (BioLegend Cat. No. 104314)
Structure: Integrin family, 110 kD
Distribution: Platelets, megakaryocytes, endothelium, smooth muscle, activated T cells, subset of B cells, monocytes, macrophages, osteoclasts, mast cells
Function: Attachment to matrix
Ligand/Receptor: Fibrinogen, von Willebrand factor, fibronectin
Cell Type: B cells, Endothelial cells, Macrophages, Mast cells, Megakaryocytes, Monocytes, Osteoclasts, Platelets, T cells
Biology Area: Cell Adhesion, Cell Biology, Immunology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook. Academic Press. 2. Phillips DR, et al. 1991. Cell. 65:359. 3. Felding-Habermann B, et al. 1993. Curr Opinion Cell Biol. 5:864.
Gene ID: 1641629302
UniProt: View information about CD61 on UniProt.org
Clone: 2C9.G2 (HMβ3-1)
Regulatory Status: RUO
Other Names: β3 integrin, Integrin β3 chain, gpIIIa
Isotype: Armenian Hamster IgG