Biolegend, 104315, APC anti-mouse/rat CD61 Antibody, 25μg

CD61 is a 110 kD integrin β chain also known as β 3 integrin or gpIIIa. It associates with the integrin α v chain (CD51) to form the vitronectin receptor. In addition, CD61 can associate with the integrin α IIb chain (CD41) to form the gpIIb/IIIa complex. CD61 is expressed on platelets, megakaryocytes, endothelium, smooth muscle, a subset of B cells, myeloid cells, osteoclasts, and mast cells. CD61, in conjunction with CD41 or CD51, mediates adhesion to fibronectin, fibrinogen, vitronectin, thrombospondin, and von Willebrand factor. Leukocyte-endothelial adhesion is mediated by the binding of α v /β 3 integrin or vitronectin receptor to CD31 (PECAM-1).
25μg
Verified Reactivity: Mouse, Rat
Antibody Type: Monoclonal
Host Species: Armenian Hamster
Immunogen: Vitronectin receptor protein from the mouse T-cell hybridoma 2B4.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Red Laser (633 nm)
Application Notes: The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 104325 and 104326).
Application References(PubMed link indicates BioLegend citation): Kieffer N, et al. 1990. Annu. Rev. Cell Biol. 6:329. (Block) Piali L, et al. 1995. J. Cell Biol. 130:451. (Block) Ashkar S, et al. 2000. Science 287:860. (Block) Schultz JF, et al. 1995. J. Biol. Chem. 270:11522. (Block) Moulder K, et al. 1991. J. Exp. Med. 173:343. (Activ) Carlson TR, et al. 2008.135:2193. PubMed Yamaji D, et al. 2009. Genes Dev. 23:2382. PubMed
Product Citations: Lan HT, et al. 2023. Evid Based Complement Alternat Med. 2022:6606423. PubMed Li X, et al. 2022. Cell. 185:1709. PubMed Mann M, et al. 2018. Cell Rep. 25:2992. PubMed Dignum T, et al. 2021. Cell Rep. 36:109675. PubMed Flommersfeld S, et al. 2021. Immunity. :. PubMed Yamamoto M, et al. 2019. Commun Biol. 2:292. PubMed Pervolarakis N, et al. 2020. Cell Rep. 108273:33. PubMed Ni T, et al. 2016. Nat Cell Biol. 18:1221-1232. PubMed Lucotti S, et al. 2019. J Clin Invest. 130:1845. PubMed
RRID: AB_2561733 (BioLegend Cat. No. 104315) AB_2561734 (BioLegend Cat. No. 104316)
Structure: Integrin family, 110 kD
Distribution: Platelets, megakaryocytes, endothelium, smooth muscle, activated T cells, subset of B cells, monocytes, macrophages, osteoclasts, mast cells
Function: Attachment to matrix
Ligand/Receptor: Fibrinogen, von Willebrand factor, fibronectin
Cell Type: B cells, Endothelial cells, Macrophages, Mast cells, Megakaryocytes, Monocytes, Osteoclasts, Platelets, T cells
Biology Area: Cell Adhesion, Cell Biology, Immunology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook. Academic Press. 2. Phillips DR, et al. 1991. Cell. 65:359. 3. Felding-Habermann B, et al. 1993. Curr Opinion Cell Biol. 5:864.
Gene ID: 1641629302
UniProt: View information about CD61 on UniProt.org
Clone: 2C9.G2 (HMβ3-1)
Regulatory Status: RUO
Other Names: β3 integrin, Integrin β3 chain, gpIIIa
Isotype: Armenian Hamster IgG