Biolegend, 104717, Alexa Fluor® 647 anti-mouse CD80 Antibody, 25μg

CD80 is a 60 kD highly glycosylated protein. It is a member of the Ig superfamily and is also known as B7-1, B7, and Ly-53. CD80 is constitutively expressed on dendritic cells and monocytes/macrophages, and inducibly expressed on activated B and T cells. The ligation of CD28 on T cells with CD80 and CD86 (B7-2) on antigen presenting cells (such as dendritic cells, macrophages, and B cells) elicits co-stimulation of T cells resulting in enhanced cell activation, proliferation, and cytokine production. CD80 appears to be expressed later in the immune response than CD86. CD80 can also bind to CD152, also known as CTLA-4, to deliver an inhibitory signal to T cells.
25μg
Verified Reactivity: Mouse
Reported Reactivity: Dog
Antibody Type: Monoclonal
Host Species: Armenian Hamster
Immunogen: CHO cell line transfected with mouse B7 (CD80)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality testedIHC-F - Verified
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. For immunohistochemistry on frozen tissue sections, a concentration range of 2.5 - 5.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation2, in vitro and in vivo blocking of CTLA-4 Ig to CD80 by blocking costimulation of T cells by activated B cells2-4, and immunohistochemical staining of acetone-fixed frozen sections1,4. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 104747-104752).
Application References(PubMed link indicates BioLegend citation): Harlan DM, et al. 1994. P. Natl. Acad. Sci. USA 91:3137. (IHC) Razi-Wolf Z, et al. 1992. P. Natl. Acad. Sci. USA 89:4210. (Block, IP) Hathcock KS, et al. 1994. J. Exp. Med. 180:631. (Block) Herold KC, et al. 1997. J. Immunol. 158:984. (Block, IHC) Ma XT, et al. 2006. Cancer Res. 66:1169. Andoniou CE, et al. 2005. Nature Immunology 6:1011. (FC) Lawson BR, et al. 2007. J. Immunol. 178:5366. Turnquist HR, et al. 2007. J. Immunol. 178:7018. Misra RS, et al. 2010. J. Exp Med. 207:1775. PubMed del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed Philipsen L, et al. 2013. Mol Cell Proteomics. 12:2551. PubMed
Product Citations: Mathur R, et al. 2019. Mucosal Immunol. 12:612. PubMed Balakrishnan PB, et al. 2022. Nano Res. 15:2300. PubMed Madar J, et al. 2023. Nat Commun. 14:2158. PubMed Piñeros AR, et al. 2022. Cell Rep. 39:111011. PubMed Lauterbach MA, et al. 2020. Immunity. 51(6):997-1011.e7.. PubMed Hu HJ, et al. 2020. Cell Death Dis. 1.168055556. PubMed Boccasavia VL, et al. 2021. Cell Reports. 34(11):108861. PubMed Shan M et al. 2018. Immunity. 49(4):709-724 . PubMed Jiang Y, et al. 2020. Adv Mater. 32:e2001808. PubMed Misra R, et al. 2010. J Exp Med. 207:1775. PubMed
RRID: AB_492825 (BioLegend Cat. No. 104717) AB_492824 (BioLegend Cat. No. 104718)
Structure: Ig superfamily, 60 kD
Distribution: Macrophages, activated B cells and T cells, dendritic cells
Function: T cell costimulation
Ligand/Receptor: CD28 (stimulatory), CD152(CTLA4) (inhibitory)
Cell Type: B cells, Dendritic cells, Macrophages, T cells, Tregs
Biology Area: Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family: CD Molecules, Immune Checkpoint Receptors
Antigen References: 1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Linsley PS, et al. 1991. J. Exp. Med. 174:561. 3. Salomon B, et al. 2001. Annu. Rev. Immunol. 19:225.
Gene ID: 12519
UniProt: View information about CD80 on UniProt.org
Clone: 16-10A1
Regulatory Status: RUO
Other Names: B7-1, B7, Ly-53
Isotype: Armenian Hamster IgG