Biolegend, 104731, Brilliant Violet 650™ anti-mouse CD80 Antibody, 125microl

CD80 is a 60 kD highly glycosylated protein. It is a member of the Ig superfamily and is also known as B7-1, B7, and Ly-53. CD80 is constitutively expressed on dendritic cells and monocytes/macrophages, and inducibly expressed on activated B and T cells. The ligation of CD28 on T cells with CD80 and CD86 (B7-2) on antigen presenting cells (such as dendritic cells, macrophages, and B cells) elicits co-stimulation of T cells resulting in enhanced cell activation, proliferation, and cytokine production. CD80 appears to be expressed later in the immune response than CD86. CD80 can also bind to CD152, also known as CTLA-4, to deliver an inhibitory signal to T cells.
125microl
Verified Reactivity: Mouse
Reported Reactivity: Dog
Antibody Type: Monoclonal
Host Species: Armenian Hamster
Immunogen: CHO cell line transfected with mouse B7 (CD80)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 650™ under optimal conditions.
Concentration: µg sizes: 0.2 mg/mLµL sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the µl size, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. For flow cytometric staining using the µg size, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.Brilliant Violet 650™ excites at 405 nm and emits at 645 nm. The bandpass filter 660/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 650™ is a trademark of Sirigen Group Ltd.Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation2, in vitro and in vivo blocking of CTLA-4 Ig to CD80 by blocking costimulation of T cells by activated B cells2-4, and immunohistochemical staining of acetone-fixed frozen sections1,4. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 104747-104752).
Application References(PubMed link indicates BioLegend citation): Harlan DM, et al. 1994. P. Natl. Acad. Sci. USA 91:3137. (IHC) Razi-Wolf Z, et al. 1992. P. Natl. Acad. Sci. USA 89:4210. (Block, IP) Hathcock KS, et al. 1994. J. Exp. Med. 180:631. (Block) Herold KC, et al. 1997. J. Immunol. 158:984. (Block, IHC) Ma XT, et al. 2006. Cancer Res. 66:1169. Andoniou CE, et al. 2005. Nature Immunology 6:1011. (FC) Lawson BR, et al. 2007. J. Immunol. 178:5366. Turnquist HR, et al. 2007. J. Immunol. 178:7018. Misra RS, et al. 2010. J. Exp Med. 207:1775. PubMed del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed Philipsen L, et al. 2013. Mol Cell Proteomics. 12:2551. PubMed
Product Citations: Hao H, et al. 2023. Nat Biomed Eng. . PubMed Tordesillas L, et al. 2018. Nat Commun. 9:5238. PubMed Mathew NR, et al. 2021. Cell Reports. 35(12):109286. PubMed Naing A et al. 2019. Cell reports. 26(5):1242-1257 . PubMed Jeong SH, et al. 2021. Nat Commun. 12:4405. PubMed Hossain DMS, et al. 2018. J Clin Invest. 128:644. PubMed Dobosz E, et al. 2021. Dis Model Mech. :14. PubMed Tacconi C, et al. 2021. Cell Reports. 35(2):108993. PubMed Pennock ND, et al. 2018. J Immunother Cancer. 0.318055556. PubMed Cosentino K, et al. 2022. Mol Cell. 82:933. PubMed Zhang Q, et al. 2021. Front Cell Dev Biol. 9:655552. PubMed Gomez S, et al. 2022. J Immunother Cancer. 10:. PubMed Lu X, et al. 2019. Circ Res. 125:1055. PubMed Zhang Q, et al. 2022. Commun Biol. 5:544. PubMed Moufarrij S, et al. 2020. Sci Rep. 10:3470. PubMed Singhal P, et al. 2016. Proc Natl Acad Sci U S A. 113: 122 - 127. PubMed Hering L, et al. 2020. Front Immunol. 1.747222222. PubMed Jones D, et al. 2015. J Immunol. 195: 4753 - 4759. PubMed
RRID: AB_11147759 (BioLegend Cat. No. 104731) AB_2686972 (BioLegend Cat. No. 104732)
Structure: Ig superfamily, 60 kD
Distribution: Macrophages, activated B cells and T cells, dendritic cells
Function: T cell costimulation
Ligand/Receptor: CD28 (stimulatory), CD152(CTLA4) (inhibitory)
Cell Type: B cells, Dendritic cells, Macrophages, T cells, Tregs
Biology Area: Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family: CD Molecules, Immune Checkpoint Receptors
Antigen References: 1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Linsley PS, et al. 1991. J. Exp. Med. 174:561. 3. Salomon B, et al. 2001. Annu. Rev. Immunol. 19:225.
Gene ID: 12519
UniProt: View information about CD80 on UniProt.org
Clone: 16-10A1
Regulatory Status: RUO
Other Names: B7-1, B7, Ly-53
Isotype: Armenian Hamster IgG