Biolegend, 105320, Alexa Fluor® 700 anti-mouse CD90.2 (Thy1.2) Antibody, 100μg

CD90.2 is a 25-35 kD immunoglobulin superfamily member also known as Thy1.2. It is expressed on hematopoietic stem cells and neurons, all thymocytes, and peripheral T cells in Thy1.2 bearing mouse strains (Balb/c, CBA/J, C3H/He, C57BL/-, DBA, NZB/-). CD90.2 is a glycosylphosphatidylinositol (GPI)-anchored membrane glycoprotein involved in signal transduction. CD90.2 is involved in costimulation of lymphocyte proliferation and induction of hematopoietic stem cells differentiation. CD90.2 has been shown to interact with CD45. The 30-H12 antibody has been reported to induce Ca 2+ flux in thymocytes and, in combination with antibody against the CD3/TCR complex, promote thymocyte apoptosis and inhibit CD3-mediated proliferative responses of mature T lymphocytes.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse thymus or spleen
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633nm / 635nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported applications (for the relevant formats) include: in vivo and in vitro depletion1,2,7, costimulation of CD3/TCR-mediated signal transduction3,4, and immunohistochemical staining5 of acetone-fixed frozen sections. The 30-H12 antibody does not react with Thy-1.1 alloantigen of the AKR/J and PL strains. To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with anti-mouse CD16/CD32, clone 93 (Cat. No. 101301 & 101302) is recommended prior to immunofluorescent staining. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 105351 & 105352).
Application References(PubMed link indicates BioLegend citation): Hathcock KS. 1991. Current Protocols in Immunology. 3.4.1. (Deplete) Seaman WE. 1983. J. Immunol. 130:1713. (Deplete) Nakashima I, et al. 1991. J. Immunol. 147:1153. (Costim) Nakashima I, et al. 1993. J. Immunol. 151:3511. (Costim) Ledbetter JA, et al. 1980. J. Exp. Med. 152:280. (IHC) Hardy B, et al. 2005. Int. Immunol. 17:615. Drobyski W, et al. 1996. Blood 87:5355. (Deplete) Dyer KD, et al. 2007. J. Immunol. 179:1693. (FC) PubMed Sungur CM, et al. 2013. PNAS. 110:7401. PubMed
Product Citations: Hirai T, et al. 2020. Immunity. 54(1):84-98.e5. PubMed Kwong Chung CK, et al. 2017. Immunology. 150:221. PubMed Christian DA, et al. 2022. Sci Immunol. 7:eabq7432. PubMed Skladanowska K, et al. 2022. Cell Rep. 41:111490. PubMed Matter AL, et al. 2022. J Immunol. 209:1880. PubMed Matsushita Y, et al. 2023. Nat Commun. 14:2383. PubMed Corral D, et al. 2022. Cell Rep. 39:110715. PubMed Giannou AD, et al. 2023. Immunity. 56:125. PubMed Yeh CH, et al. 2022. Immunity. 55:272. PubMed Potempa M, et al. 2022. J Immunol. 208:1742. PubMed Krausgruber T, et al. 2020. Nature. 583:296. PubMed Herz J, et al. 2015. J Exp Med. 212: 1153 - 1169. PubMed Riether C, et al. 2021. Cell Reports. 34(4):108663. PubMed AR P, et al. 2016. Circ Res. 118: 400-409. PubMed Panciera T, et al. 2016. Cell Stem Cell. 19:725-737. PubMed Dikiy S, et al. 2021. Immunity. 54(5):931-946.e11. PubMed Emgård J, et al. 2018. Immunity. 143:419. PubMed Watanabe M, et al. 2020. Nat Commun. 4.808333333. PubMed Schuldt N, et al. 2015. PLoS One. 10: 0145762. PubMed Seedhom M, et al. 2012. J Virol. 86:6010. PubMed Lu P, et al. 2021. Nat Commun. 12:1042. PubMed Monticelli L, et al. 2015. Proc Natl Acad Sci U S A. 112: 10762-10767. PubMed Toshiro Hirai et al. 2019. Immunity. 50(5):1249-1261 . PubMed Kalafati L, et al. 2020. Cell. 183:771. PubMed Williams JB, et al. 2020. Nat Commun. 0.876388889. PubMed Rive CM, et al. 2022. Mol Ther Methods Clin Dev. 26:4. PubMed Zeis P, et al. 2020. Immunity. 53:775. PubMed Nagashima H et al. 2019. Immunity. 51(4):682-695 . PubMed Urata S, et al. 2018. PLoS Pathog. 14:e1007172. PubMed Baumann D, et al. 2020. Nat Commun. 1.969444444. PubMed Di Pilato M, et al. 2021. Cell. 184(17):4512-4530.e22. PubMed Teng F, et al. 2021. Cell Rep. 37:110051. PubMed Goc J, et al. 2021. Cell. 184:5015. PubMed
RRID: AB_493724 (BioLegend Cat. No. 105319) AB_493725 (BioLegend Cat. No. 105320)
Structure: Ig superfamily, 25-35 kD
Distribution: Hematopoietic stem cells and neurons, all thymocytes, peripheral T cells of the Thy-1.2 bearing mice
Function: Lymphocyte costimulation, proliferation and differentiation of hematopoietic stem cells
Ligand/Receptor: CD45
Cell Type: Hematopoietic stem and progenitors, Neurons, T cells, Thymocytes
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Craig W, et al. 1993. J. Exp. Med. 177:1331. 3. Reif AE and Schlesinger M. 1989. Cell Surface Antigen Thy-1. 4. Mayani H, et al. 1994. Blood 83:2410.
Gene ID: 21838
UniProt: View information about CD90.2 on UniProt.org
Clone: 30-H12
Regulatory Status: RUO
Other Names: Thy-1.2
Isotype: Rat IgG2b, κ