Biolegend, 105403, PE anti-mouse/human Helios Recombinant Antibody, 25tests

Helios is a member of the Ikaros family of zinc finger transcription factors. It contains a C-terminal region composed of 2 zinc-finger domains that mediate dimerization between the family members. Helios was originally cloned from a mouse thymoma line. It is mainly expressed in peripheral T cells and thymocytes. It is found at high levels in a subpopulation of regulatory T cells. Helios plays an important role in T cell development and homeostasis. Overexpression of Helios profoundly alters αβ T cell differentiation and activation. It has been determined that silencing of Helios in B cells is critical for maintaining normal B cell function. Helios is also involved in tumor immunity.
25tests
Verified Reactivity: Mouse, Human
Antibody Type: Recombinant
Host Species: Rat
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Clone QA20A52 is able to completely block the staining of clone 22F6 on target cells
RRID: AB_2894413 (BioLegend Cat. No. 105403) AB_2894413 (BioLegend Cat. No. 105404)
Structure: A zinc finger nuclear transcription factor of the Ikaros family, containing 2 zinc-finger domains in C-terminal region.
Distribution: Regulatory T cells (Tregs), conventional T cells, thymocytes.
Function: Plays an important role in T cell development and homeostasis, involved in tumor immunity.
Cell Type: T cells, Thymocytes, Tregs
Biology Area: Adaptive Immunity, Cell Biology, Immunology, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: 1. Kelly CM, et al. 1998. Curr Biol. 8:508. 2. Dovat S, et al. 2005. J Immunol. 175:3508. 3. Cortes M, et al. 1999. Curr Opin Immunol. 11:167. 4. Cai Q, et al. 2009. J Immunol. 183:2303. 5. Zhang Z, et al. 2007. Blood. 109:2190. 6. Hahm K, et al. 1998. Genes Dev. 12:782.
Gene ID: 2277922807
UniProt: View information about Helios on UniProt.org
Clone: QA20A52
Regulatory Status: RUO
Other Names: IKZF2, IKAROS family zinc finger 2, ANF1A2, ZNF1A2, ZNFN1A2
Isotype: Rat IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.