Biolegend, 105513, PerCP/Cyanine5.5 anti-mouse CD94 Antibody, 25μg

CD94 is a 43/39 kD C-type lectin, also known as Kp43. It is present on all NK cells, NKT cells, and a subset of CD8-positive T lymphocytes in most mouse strains. CD94 is a type-II transmembrane protein with an extracellular lectin-like domain and a short cytoplasmic tail. CD94 is expressed as a disulphide-linked heterodimer with a NKG2 subunit believed to mediate signal transduction. When associated with NKG2A, the complex triggers inhibition; when associated with NKG2C, the complex triggers stimulation. The receptor complex of CD94 and NKG2 receptors bind to the ligand, Qa-1, and are thought to play a role in maintaining self-tolerance in developing NK cells.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: CHO cells expressing the C57BL/6 alleles of CD94 and NKG2A
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser: Blue Laser (488 nm)
Application References(PubMed link indicates BioLegend citation): Vance RE, et al. 1999. J. Exp. Med. 190:1801. Tang X, et al. 2006. J. Immunol. 177:7645. PubMed
RRID: AB_2810341 (BioLegend Cat. No. 105513) AB_2810342 (BioLegend Cat. No. 105514)
Disclaimer: This product may be used for research purposes only. It is not licensed for resale and may only be used by the buyer. This product may not be used and is not licensed for clinical assays where the results of such assays are provided as a diagnostic service. If a diagnostic or therapeutic use is anticipated then a license must be requested from the University of California. The availability of such diagnostic and therapeutic use license(s) cannot be guaranteed from the University of California.
Structure: C-type lectin; 43/39 kD
Distribution: NK cells, NK-T cells, CD8+ T subset
Function: Inhibits NK cell function
Ligand/Receptor: CD94/NKG2
Cell Type: NK cells, NKT cells, T cells
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules
Antigen References: Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. Lopez-Botet M, et al. 1997. Immunol. Rev. 155:165. Moretta A, et al. 1997. Immunol. Rev. 155:105. Phillips JH, et al. 1996. Immunity 5:163.
Gene ID: 16643
UniProt: View information about CD94 on UniProt.org
Clone: 18d3
Regulatory Status: RUO
Other Names: Kp43
Isotype: Rat IgG2a, κ
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.