Biolegend, 105611, Alexa Fluor® 647 anti-mouse CD102 Antibody, 25μg

CD102 is a 55 kD immunoglobulin superfamily member, also known as ICAM-2. CD102 is a heavily glycosylated cell surface ligand for LFA-1 (CD11a/CD18). CD102 is constitutively expressed on vascular endothelial cells, some lymphocytes, monocytes, and platelets. CD102 is thought to be involved in lymphocyte recirculation and T cell activation. The 3C4(MIC2/4) antibody can block interactions between ICAM-2 and LFA-1.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: COS cells transfected with mouse ICAM-2 cDNA
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining2 of acetone-fixed frozen sections, in vitro blocking of ligand binding1, and immunoprecipitation1. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 105608).
Application References(PubMed link indicates BioLegend citation): Xu H, et al. 1996. J. Immunol. 156:4909. Gerwin N, et al. 1999. Immunity 10:9.
Product Citations: Dobie R, et al. 2020. Cell Reports. 29(7):1832-1847.e8.. PubMed Cohen K, et al. 2021. Cell Rep. 37:110026. PubMed Regev O, et al. 2022. Front Immunol. 13:849701. PubMed Minutti CM, et al. 2019. Immunity. 50:645. PubMed Biram A, et al. 2020. Cell Rep. 30:1910. PubMed De Niz M, et al. 2021. Cell Rep. 36:109741. PubMed Matthew B Buechler et al. 2019. Immunity. 51(1):119-130 . PubMed Souza C, et al. 2012. J Biol Chem. 287:43810. PubMed De Niz M, et al. 2022. STAR Protoc. 3:101450. PubMed
RRID: AB_2122183 (BioLegend Cat. No. 105611) AB_2122182 (BioLegend Cat. No. 105612)
Structure: Ig superfamily, 55 kD
Distribution: Vascular endothelial cells, some resting lymphocytes, monocytes, platelets
Function: Lymphocyte recirculation, costimulation
Ligand/Receptor: LFA-1, Mac-1
Cell Type: Endothelial cells, Lymphocytes, Monocytes, Platelets
Biology Area: Cell Adhesion, Cell Biology, Immunology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Casasnovas JM, et al. 1999. P. Natl. Acad. Sci. USA 96:3017. 3. Diamond MS, et al. 1990. J. Cell Biol. 111:3129.
Gene ID: 15896
UniProt: View information about CD102 on UniProt.org
Clone: 3C4 (MIC2/4)
Regulatory Status: RUO
Other Names: ICAM-2
Isotype: Rat IgG2a, κ