Biolegend, 105805, FITC anti-mouse CD117 (c-Kit) Antibody, 50μg

CD117 is a 145 kD immunoglobulin superfamily member also known as c-Kit and stem cell factor receptor (SCFR). It is a transmembrane tyrosine-kinase receptor that binds the c-Kit ligand (also known as steel factor, stem cell factor, and mast cell growth factor). CD117 is expressed on hematopoietic stem cells (including multipotent hematopoietic stem cells, progenitors committed to myeloid and/or erythroid lineages, and T and B cell precursors), mast cells, and acute myeloid leukemia (AML) cells. CD117 interaction with its ligand is critical for the development of hematopoietic stem cells.
50μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse bone marrow mast cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of acetone fixed frozen sections2, and spatial biology (IBEX)5,6. The 2B8 antibody does not block c-Kit activity.
Application References(PubMed link indicates BioLegend citation): Ikuta K, et al. 1992. P. Natl. Acad. Sci. USA 89:1502. (FC) Podd BS, et al. 2006. J. Immunol. 176:6532. PubMed (IHC) Bachelet I, et al. 2008. J. Immunol. 180:6064. PubMed (FC) Charles N, et al. 2010. Nat. Med. 16:701. PubMed (FC) Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations: Petenkova A, et al. 2023. Nat Commun. 14:2761. PubMed Wu J, et al. 2022. Stem Cells Int. 2022:4249843. PubMed Roberts JL, et al. 2022. Bone. 157:116310. PubMed Chen W, et al. 2020. Cell Discov. 6:56. PubMed Adamiak M, et al. 2015. Leukemia. . PubMed Mann M, et al. 2018. Cell Rep. 25:2992. PubMed Chen W, et al. 2020. Stem Cell Reports. 1.140972222. PubMed Timofeev O, et al. 2020. Cancer Res. 80:5231. PubMed Diesner S, et al. 2012. Immunol Lett. 141:210. PubMed Nosho S, et al. 2020. Int J Mol Sci. 21:00. PubMed Duan Y, et al. 2018. Stem Cells. 36:1430. PubMed Murakami K, et al. 2021. Cell Reports. 34(1):108579. PubMed Mitry MA, et al. 2020. American Journal of Physiology-Cell Physiology. 318(2):C380-C391. PubMed Chen W, et al. 2020. Cell Discov. 6:56. PubMed Gough D, et al. 2014. Blood. 124:2252. PubMed Brusilovsky M, et al. 2021. Nat Immunol. 22:1316. PubMed Liu B, et al. 2013. Development. 140:780. PubMed Cantor DJ et al. 2019. Cell reports. 26(1):108-118 . PubMed Lenkiewicz AM, et al. 2019. Stem Cell Rev. 0.896527778. PubMed Rodríguez L, et al. 2021. Biomolecules. 11: . PubMed Halvarsson C, et al. 2019. Antioxid Redox Signal. 31:211. PubMed Lyons DO, et al. 2018. PLoS One. 13:e0207704. PubMed Zhang B, et al. 2021. Nature. 599:471. PubMed Jatho A, et al. 2022. Cells. 11:. PubMed
RRID: AB_313214 (BioLegend Cat. No. 105805) AB_313215 (BioLegend Cat. No. 105806)
Structure: Ig superfamily, 145 kD
Distribution: Hematopoietic stem cells, AML, mast cells
Function: Growth factor receptor, tyrosine kinase
Ligand/Receptor: Stem Cell Factor (SCF)
Cell Type: Embryonic Stem Cells, Hematopoietic stem and progenitors, Leukemia, Mast cells, Mesenchymal Stem Cells
Biology Area: Immunology, Stem Cells
Molecular Family: CD Molecules
Antigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Galli SJ, et al. 1994. Adv. Immunol. 55:1. 3. Ikuta K, et al. 1992. Annu. Rev. Immunol. 10:759. 4. Besmer P, et al. 1986. Nature 320:415. 5. Witte ON. 1990. Cell 63:5.
Gene ID: 16590
UniProt: View information about CD117 on UniProt.org
Clone: 2B8
Regulatory Status: RUO
Other Names: c-KIT, Stem Cell Factor Receptor (SCFR)
Isotype: Rat IgG2b, κ