Biolegend, 106109, APC anti-mouse CD137 Antibody, 25μg

CD137 is a 39 kD TNF-receptor superfamily member also known as 4-1BB, TNFRSF9, Ly-63, and ILA (Induced by Lymphocyte Activation). CD137 is expressed as monomers, dimers, and tetramers on activated T and B cells. Interaction of CD137 with its ligand, 4-1BBL (CD137L), is important in T-B cell costimulation. The 17B5 antibody has been reported to block 4-1BBL costimulated T cell proliferation.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Syrian Hamster
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported applications (for the relevant formats) include: in vitro blocking of 4-1BB-mediated T cell proliferation2. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 106108).
Application References(PubMed link indicates BioLegend citation): Lee SW, et al. 2005. J. Immunol. 174:6803. (FC) Zheng G, et al. 2004. J. Immunol. 173:2428. (Block) Zheng H and G. G. Meadows 2005. J. Leukocyte Biol. 78:1070. (FC) PubMed Nishimoto H, et al. 2005. Blood 106:4241. (FC) del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
Product Citations: van Elsas MJ, et al. 2023. J Immunother Cancer. 11:. PubMed Duraiswamy J, et al. 2021. Cancer Cell. 39:1623. PubMed Huang Z et al. 2017. Cell metabolism. 26(3):493-508 . PubMed Ford K, et al. 2020. Cancer Res. 80:1846. PubMed Chow MT et al. 2019. Immunity. 50(6):1498-1512 . PubMed Qi X, et al. 2019. Nat Commun. 10:2141. PubMed Potluri HK, et al. 2022. J Immunother Cancer. 10:. PubMed Etxeberria I, et al. 2020. Cancer Cell. 36(6):613-629. PubMed Wang C, et al. 2021. Cell Rep. 37:110021. PubMed Palazon A, et al. 2017. Cancer Cell. . 10.1016/j.ccell.2017.10.003. PubMed
RRID: AB_2564296 (BioLegend Cat. No. 106109) AB_2564297 (BioLegend Cat. No. 106110)
Disclaimer: This product may be used for research purposes only. It is not licensed for resale and may only be used by the buyer. This product may not be used and is not licensed for clinical assays where the results of such assays are provided as a diagnostic service. If a diagnostic or therapeutic use is anticipated then a license must be requested from the University of California. The availability of such diagnostic and therapeutic use license(s) cannot be guaranteed from the University of California.
Structure: TNF-R superfamily, 39 kD
Distribution: Activated T cells and B cells
Function: T-B cell costimulation
Ligand/Receptor: 4-1BB ligand (CDw137L)
Cell Type: B cells, T cells, Tregs
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Alderson MR, et al. 1994. Eur. J. Immunol. 24:2219. 3. Schwarz H, et al. 1996. Blood 87:2839.
Gene ID: 21942
UniProt: View information about CD137 on UniProt.org
Clone: 17B5
Regulatory Status: RUO
Other Names: 4-1BB, TNFRSF9, Ly-63, ILA (Induced by Lymphocyte Activation), CD137
Isotype: Syrian Hamster IgG