Biolegend, 106208, GoInVivo™ Purified anti-mouse CD152 Antibody, 100mg

CD152 is a 33 kD member of the immunoglobulin superfamily also known as CTLA-4 or Ly-56. It is expressed on activated T and B lymphocytes. CD152 is similar to CD28 in amino acid sequence, structure, and genomic organization and these two receptors share common B7 family counter-receptors (B7-1, B7-2). Whereas CD28 delivers a costimulatory signal in T cell activation, CTLA-4 negatively regulates cell-mediated immune responses. CD152 is thought to play a role in the induction and maintenance of immunological tolerance, as well as the development of protective immunity and thymocyte regulation. The 9H10 antibody can enhance T cell co-stimulation by blocking CTLA-4 interactions with the B7 co-receptors, favoring CD28 interactions.
100mg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Syrian Hamster
Immunogen: Mouse CTLA-4-human IgG1 fusion protein
Formulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level: Less than 1.0 EU/mg of the protein (< 0.1 pg/µg of the protein) as determined by the LAL test.
Preparation: The GoInVivo™ antibody was purified by affinity chromatography.
Concentration: The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. This GoInVivo™ solution contains no preservative; handle under aseptic conditions.
Application: ELISA - Quality tested Block, Costim, FC - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. For in vivo and in vitro applications, we recommend to perform a pilot experiment to determine the optimal concentration to use for each particular experiment.
Application Notes: Additional reported applications (for the relevant formats) include: in vivo and in vitro blocking1-4 of CTLA-4 action by soluble antibody and promotion of in vitro CTLA-4 signalling by immobilized antibody1. To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with anti-mouse CD16/CD32, clone 93 (Cat. No. 101301/101302), is recommended prior to immunofluorescent staining. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-Syrian hamster IgG (Cat. No. 405601) second step, followed by SAv-PE (Cat. No. 405204)).
Additional Product Notes: GoInVivo™ products are guaranteed to be pathogen-free based on the IDEXX BioResearch IMPACT test via PCR. For a full listing of pathogens tested, visit the GoInVivo™ Webpage.
Application References(PubMed link indicates BioLegend citation): Krummel MF, et al. 1995. J. Exp. Med. 182:459. (Block, Costim, FC) Krummel MF, et al. 1996. Int. Immunol. 8:519. (Block) Leach DR, et al. 1996. Science 271:1734. (Block) Wagner Jr, DH, et al. 1996. J. Exp. Med. 184:1631. (Block)
Product Citations: Wang Y, et al. 2016. Exp Ther Med. 81:12. PubMed Zhu Y, et al. 2023. Sci Immunol. eade4656:8. PubMed Zhao Y, et al. 2023. Cell Metab. 1688:35. PubMed Murphy S, et al. 2024. Cancer Res. 1597:84. PubMed
RRID: AB_2616629 (BioLegend Cat. No. 106205) AB_2616630 (BioLegend Cat. No. 106206) AB_2616631 (BioLegend Cat. No. 106207) AB_2616632 (BioLegend Cat. No. 106208) AB_2616633 (BioLegend Cat. No. 106209) AB_2616634 (BioLegend Cat. No. 106210)
Disclaimer: This product may be used for research purposes only. It is not licensed for resale and may only be used by the buyer. This product may not be used and is not licensed for clinical assays where the results of such assays are provided as a diagnostic service. If a diagnostic or therapeutic use is anticipated then a license must be requested from the University of California. The availability of such diagnostic and therapeutic use license(s) cannot be guaranteed from the University of California.
Structure: Ig superfamily, 33 kD
Distribution: Activated T cells
Function: Negative regulator of T cell activation
Ligand/Receptor: CD80 (B7-1), CD86 (B7-2)
Cell Type: T cells, Tregs
Biology Area: Immunology
Molecular Family: CD Molecules, Immune Checkpoint Receptors
Antigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Allison JP, et al. 1995. Science 270:932. 3. Waterhouse P, et al. 1995. Science 270:985. 4. Linsley PS, et al. 1991. J. Exp. Med. 174:561.
Gene ID: 12477
UniProt: View information about CD152 on UniProt.org
Clone: 9H10
Regulatory Status: RUO
Other Names: Cytotoxic T Lymphocyte-Associated Antigen-4 (CTLA-4), Ly-56
Isotype: Syrian Hamster IgG