Biolegend, 107504, FITC anti-mouse TCR γ/δ Antibody, 500μg

T cell receptor (TCR) is a heterodimer consisting of an α and a β chain (TCR α/β) or a γ and a δ chain (TCR γ/δ). TCR γ/δ belongs to the immunoglobulin superfamily, involved in the recognition of certain bacterial and tumor antigens bound to MHC class I. The TCR γ/δ associates with CD3 and is expressed on a T cell subset found in the thymus, the intestinal epithelium, and the peripheral lymphoid tissues and peritoneum. Most γ/δ T cells are CD4-/CD8-, some are CD8+. T cells expressing the TCR γ/δ have been shown to play a role in oral tolerance, tumor-associated tolerance, and autoimmune disease. It has been reported that γ/δ T cells also play a principal role in antigen presentation. Immobilized UC7-13D5 antibody has been reported to activate TCR-γ/δ-bearing T cells in vitro , and to deplete peripheral TCR-γ/δ-bearing T cells in vivo .
500μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Armenian Hamster
Formulation: Tris-buffered solution pH 8.0, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro stimulation1,2,4. Although several papers have reported in vivo depletion3, 5,6, another study found that the effect of the antibody could be the functional blocking of the TCR signaling, rather than cell depletion7. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 107515 - 107520). Though clone UC7-13D5 has been used for the depletion of TCR γ/δ T cells in vivo3-5, there is a report suggesting that binding of clone UC7-13D5 to the γ/δ TCR leads to downregulation of the γ/δ TCR, making the γ/δ T cells undetectable although they are still present7.
Application References(PubMed link indicates BioLegend citation): Bluestone JA, et al. 1991. Immunol. Rev. 120:5. (IP Costim) Kruisbeek AM, et al. 1991. In Current Protocols in Immunology p. 3.12.1 - 3.12.14. (Costim) Hiromatsu K, et al. 1992. J. Exp. Med. 175:49. (Deplete) Dieli F, et al. 1997. Eur. J. Immunol. 27:206. (Costim) van der Heyde HC, et al. 1995. J. Immunol. 154:3985. (Deplete) Pollinger B, et al. 2011. J. Immunol. 186:2602. PubMed Koenecke C, et al. 2009. Eur J Immunol. 39(2):372-9.
Product Citations: Pandey SP, et al. 2022. Cell Host Microbe. 30:1003. PubMed Gravano D, et al. 2010. PLoS One. 5:e13528. PubMed Köchl R, et al. 2020. Elife. 9:00. PubMed Du Y, et al. 2022. Nat Commun. 13:231. PubMed Dmitrieva‐Posocco O et al. 2019. Immunity. 50(1):166-180 . PubMed Ma S, et al. 2022. Cell Rep. 38:110520. PubMed Jin C, et al. 2019. Cell. 176:998. PubMed Espinal A, et al. 2022. Int J Mol Sci. 23:. PubMed Khosravi N, et al. 2018. Cancer Immunol Res. 0.797222222. PubMed Wu J, et al. 2017. J Med Virol. 10.1002/jmv.24914. PubMed Burton OT, et al. 2018. Clin Exp Allergy. 48:288. PubMed
RRID: AB_313312 (BioLegend Cat. No. 107503) AB_313313 (BioLegend Cat. No. 107504)
Structure: Ig superfamily, associates with CD3 complex
Distribution: T subset in thymus, intestinal epithelium, peripheral lymphoid tissues and peritoneum
Function: Antigen recognition
Ligand/Receptor: Some bacterial or tumor antigens bound MHC class I
Cell Type: Epithelial cells, T cells, Tregs
Biology Area: Adaptive Immunity, Immunology
Molecular Family: TCRs
Antigen References: 1. Skarstein K, et al. 1995. Immunology 81:497. 2. Harrison LC, et al. 1996. J. Exp. Med. 184:2167. 3. Wildner G, et al. 1996. Eur. J. Immunol. 26:2140. 4. Brandes M, et al. 2005. Science 309:264.
Gene ID: 110066110067
UniProt: View information about TCR gamma/delta on UniProt.org
Clone: UC7-13D5
Regulatory Status: RUO
Other Names: TCR-γ/δ, γ/δ TCR
Isotype: Armenian Hamster IgG