Biolegend, 107715, PE/Fire™ 700 anti-mouse CD278 (ICOS) Antibody, 25μg

CD278 is a 47-57 kD protein, also known as inducible costimulatory molecule (ICOS), or H4. This protein is homologous to the CD28/CTLA-4 proteins. It forms homodimers and is expressed on activated T cells and a subset of thymocytes. It is able to costimulate T cells proliferation. In addition, ICOS has been shown to be involved in humoral immune responses (B cell germinal center formation). The ICOS ligand is B7-H2. ICOS stimulation has been shown to potentiate TCR-mediated IL-4 and IL-10 production and has been proposed to play a role in Th 2 cell development. It has also been shown to be involved in airway tolerance and the downregulation of pulmonary inflammation.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Syrian Hamster
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Fire™ 700 under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. * PE/Fire™ 700 has a maximum excitation of 565 nm and a maximum emission of 695 nm.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining2 of acetone-fixed frozen sections.
Application References(PubMed link indicates BioLegend citation): Dong C, et al. 2001. Nature 409(6816):97. Walker LSK, et al. 2003. J. Immunol. 170:91.
RRID: AB_2924446 (BioLegend Cat. No. 107715) AB_2924446 (BioLegend Cat. No. 107716)
Structure: CD28/CTLA-4, 47-57kD
Distribution: Activated T cells, subset of thymocytes
Function: Costimulates T cell activation, proliferation, humoral immune response
Ligand/Receptor: B7h/B7RP-1/GL-50
Cell Type: T cells, Thymocytes, Tregs
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: Rudd CE, et al. 2003. Nat. Rev. Immunol. 3:544. McAdam AJ, et al. 2000. J. Immunol. 165:5035. Mak TW, et al. 2003. Nat. Immunol. 4:765
Gene ID: 54167
UniProt: View information about CD278 on UniProt.org
Clone: 15F9
Regulatory Status: RUO
Other Names: Inducible COStimulatory molecule, H4, CD278
Isotype: Syrian Hamster IgG