Biolegend, 108119, Pacific Blue™ anti-mouse Ly-6A/E (Sca-1) Antibody, 25μg

Ly-6A/E, also known as Sca-1, is an 18 kD member of the Ly-6 multigene family. Ly6A/E is a glycosylphosphatidylinositol (GPI)-linked protein expressed on hematopoietic stem cells. In mice expressing the Ly-6.2 haplotype (e.g., AKR, C57BL, C57BR, DBA/2, SJL, SWR, and 129), Ly-6A/E is also expressed on peripheral B lymphocytes and thymic and peripheral T lymphocytes. Strains expressing the Ly-6.1 haplotype (e.g., BALB/c, CBA, C3H/He, DBA/1, and NZB) have low Ly-6A/E expression on resting peripheral lymphocytes. The expression of Ly-6A/E on lymphocytes is upregulated upon activation from both Ly6.1 and Ly6.2 haplotype mice. Ly-6A/E is thought to be involved in the regulation of both T and B cell responses.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: IL-2-dependent mouse T-cell line (CTL-L)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The Ly-6A/E antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Violet Laser (405 nm)
Application Notes: The D7 antibody has been reported to induce T cell activation and inhibit TCR-induced IL-2 production. Additional reported applications (for the relevant formats) include: Western blotting1,2, immunoprecipitation1, in vitro lymphocyte activation3-6, induction of redirected lysis7, induction of T cell inhibitory signalling8, immunofluorescence9, and immunohistochemical staining of acetone-fixed frozen sections13 and Bouin-fixed, paraffin-embedded samples9. The two Sca-1 recognizing clones D7 and E13-161.7 have been shown to bind distinct epitopes due to the inability of D7 to block the binding of E13-161.7.14
Application References(PubMed link indicates BioLegend citation): Ortega G, et al. 1986. J. Immunol. 137:3240. (WB, IP) Palfree RGE, et al. 1986. Immunogenetics 23:197. (WB) Codias EK, et al. 1990. J. Immunol. 144:2197. Malek TR, et al. 1986. J. Exp. Med. 164:709. Codias EK, et al. 1990. J. Immunol. 145:1407. Ivanov V, et al. 1994. J. Immunol. 153:2394. Karlhofer FM, et al. 1991. J. Immunol. 146:3662. Fleming T, et al. 1994. J. Immunol. 153:1955. van Bragt MPA, et al. 2005. Biol. Reprod. 73:634. (IF, IHC) Umland O, et al. 2007. J. Immunol. 178:4147. Cridland SO, et al. 2009. Blood Cell. Mol. Dis. 45:149. (FC) PubMed Pronk CJ, et al. 2011. J. Exp Med. PubMed English A, et al. 2000. J. Immunol. 165:3763. (IHC) Bamezai A and Rock KL. 1995. Proc. Natl. Acad. Sci. USA 92:4294. Wiesner DL, et al. 2015. PLoS Pathog. 11:1004701. PubMed
Product Citations: Pietras E, et al. 2016. Nat Cell Biol. 10.1038/ncb3346. PubMed Luchsinger LL, et al. 2020. Cell Stem Cell. 25(2):225-240. PubMed Aguilo F, et al. 2011. Blood. 117:5057. PubMed Cheong LY, et al. 2023. Nat Commun. 14:1213. PubMed Kang YA, et al. 2023. J Exp Med. 220:. PubMed Ho TT, et al. 2021. J Exp Med. 218:. PubMed Omer-Javed A, et al. 2022. Cell. 185:2248. PubMed Dolfi B, et al. 2022. Cell Rep. 39:110949. PubMed Vasquez EG, et al. 2022. Cell Stem Cell. 29:1213. PubMed Abe I, et al. 2022. Dev Cell. 57:2623. PubMed Kraemer AN, et al. 2022. Front Immunol. 13:933191. PubMed Qiu J, et al. 2022. STAR Protoc. 3:101828. PubMed Pisterzi P, et al. 2023. Hemasphere. 7:e823. PubMed Ernst MPT, et al. 2023. Hemasphere. 7:e824. PubMed Huang Z et al. 2017. Cell metabolism. 26(3):493-508 . PubMed Florentin J, et al. 2022. Front Immunol. 13:882484. PubMed Søndergaard E, et al. 2020. Cell Reports. 29(9):2756-2769.e6.. PubMed Ambrosi TH, et al. 2021. Nature. 597:256. PubMed Dusseault J, et al. 2016. Diabetes. 65: 2652 - 2666. PubMed Giordani L, et al. 2020. Molecular Cell. 74(3):609-621.e6.. PubMed Zhou S, et al. 2020. Cell Res. 30:1063. PubMed Wosczyna MN, et al. 2021. Cell Stem Cell. 28(7):1323-1334.e8. PubMed Gao B, et al. 2018. Aging Cell. 3. PubMed Mann M, et al. 2018. Cell Rep. 25:2992. PubMed Dave K et al. 2017. eLife. 6 pii: e23382. PubMed Grassinger J, et al. 2009. Blood. 114:49:00. PubMed LeBlond ND, et al. 2020. J Lipid Res. 61:1697. PubMed Xiong X et al. 2018. Cell reports. 25(11):3006-3020 . PubMed Khateb M, et al. 2022. Cell Rep. 40:111219. PubMed Joshi PA, et al. 2019. Nat Commun. 10:1760. PubMed Wosczyna MN et al. 2019. Cell Rep. 27(7):2029-2035 . PubMed Hillel–Karniel C, et al. 2020. Cell Reports. 30(3):807-819.e4.. PubMed Di Genua C, et al. 2020. Cancer Cell. 37(5):690-704.e8.. PubMed Quarta M, et al. 2016. Nat Biotechnol. 10.1038/nbt.3576. PubMed Morales-Mantilla DE, et al. 2022. Elife. 11:. PubMed Kenswil KJG, et al. 2021. Cell Stem Cell. 28(4):653-670.e11. PubMed Nemeth M, et al. 2009. Stem Cells. 27:1109. PubMed Lebert-Ghali C, et al. 2016. Blood. 127: 87-90. PubMed Oguri Y, et al. 2020. Cell. 182(3):563-577.e20. PubMed Jonas B, et al. 2016. PLoS One. 11: 0159189. PubMed Chen Z, et al. 2019. J Exp Med. 216:152. PubMed Chu X, et al. 2021. Front Cell Dev Biol. 9:671841. PubMed Mueller A, et al. 2016. Nature. 540:276-279. PubMed Liang R, et al. 2020. Cell Stem Cell. 359:26. PubMed Koelwyn GJ, et al. 2020. Nat Med. 1452:26. PubMed Karamitros D, et al. 2015. Development. 142:70. PubMed Torreggiani E, et al. 2013. PLoS One. 8:75204. PubMed Haider N, et al. 2020. Cell Commun Signal. 18:26. PubMed Vasamsetti SB, et al. 2018. Immunity. 49:93. PubMed Barrett TJ, et al. 2021. Cell Rep. 36:109595. PubMed Wu X, et al. 2018. Cell. 172:423. PubMed Di Genua C, et al. 2019. Haematologica. 104:2215. PubMed LeBlond ND, et al. 2020. J Lipid Res. 61:1697. PubMed Caputo T, et al. 2020. Cell Mol Life Sci. . PubMed Alvarez S, et al. 2015. Nat Commun. 6: 8548. PubMed Sabnis A, et al. 2009. PLoS One. 7:e59. PubMed Agarwal A, et al. 2008. Blood. 112:1960. PubMed He M, et al. 2020. Cell Metabolism. 31(3):580-591. PubMed Liu L et al. 2018. Cell stem cell. 23(4):544-556 . PubMed Ambrosio F, et al. 2014. Free Radic Biol Med. 74:64. PubMed Viaud M, et al. 2020. Cell Reports. 30(10):3397-3410. PubMed Yamashita M, et al. 2019. Cell Stem Cell. 25:357. PubMed Halvarsson C, et al. 2019. Antioxid Redox Signal. 31:211. PubMed Luo H, et al. 2019. Cell Rep. 26:945. PubMed Sarshad AA, et al. 2018. Mol Cell. 71:1040. PubMed Yue F, et al. 2022. Cell Rep. 38:110267. PubMed Kang YA, et al. 2020. J Exp Med. 217:00:00. PubMed Guarnerio J, et al. 2018. Nat Commun. 9:66. PubMed
RRID: AB_493274 (BioLegend Cat. No. 108119) AB_493273 (BioLegend Cat. No. 108120)
Structure: Ly-6 multigene family, 18 kD
Distribution: Hematopoietic stem cells, activated T cells and B cells, subset of resting B cells and T cells
Function: Regulates B and T cell responses
Cell Type: B cells, Hematopoietic stem and progenitors, Mesenchymal Stem Cells, T cells
Biology Area: Immunology, Stem Cells
Antigen References: 1. Rock KL, et al. 1989. Immunol. Rev. 111:195. 2. Morrison SJ, et al. 1994. Immunity 1:661. 3. Spangrude GJ, et al. 1988. J. Immunol. 141:3697. 4. Malek T, et al. 1986. J. Exp. Med. 164:709.
Gene ID: 110454
UniProt: View information about Ly-6A/E on UniProt.org
Clone: D7
Regulatory Status: RUO
Other Names: Sca-1
Isotype: Rat IgG2a, κ