Biolegend, 108209, PE/Cyanine7 anti-mouse Ly-49C/F/I/H Antibody, 25μg

The 14B11 antibody reacts with a common epitope of Ly-49C/F/I/H family members also known as Ly-49 inhibitory receptors C, F, and I and activating receptor H. It does not react with other Ly-49 members (i.e., Ly-49A, B, D, and G). Ly-49 is a type-II transmembrane, disulfide-linked homodimer with C-type lectin extracellular domains that binds to MHC class I. Ly-49C/F/I/H is expressed on a subset of NK cells, NK-T cells and some CD8 + cells. Ly-49C/F/I, like other members of this family, inhibits NK cell activation through the recognition and interaction of certain MHC class I haplotypes on potential target cells. Ly-49C, Ly-49F, and Ly-49I contain an ITIM motif in their cytoplasmic tails, while Ly-49H lacks ITIM. Ly-49H is encoded by the Cmv1r gene, which promotes resistance to infection by mouse cytomegalovirus (CMV). BALB/c mice are reported to lack the Ly-49H and Ly-49I receptors. The 14B11 antibody, when bound to NK cells, is capable of inducing redirected cell lysis and blocking NK cell effector function.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Syrian Hamster
Immunogen: IL-2-activated killer cells (LAK) from C57BL/6 mice
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications (for the relevant formats) include: induction of redirected lysis1, and blocking2 of the binding of H-2d lymphoblasts to transfectants expressing Ly-49C, Ly-49F, and Ly-49I.
Application References(PubMed link indicates BioLegend citation): Corral L, et al. 1999. Hybridoma 18:359. Hanke T, et al. 1999. Immunity 11:67.
Product Citations: Kragten NAM, et al. 2022. Eur J Immunol. 52:389. PubMed
RRID: AB_2728130 (BioLegend Cat. No. 108209) AB_2728131 (BioLegend Cat. No. 108210)
Disclaimer: This product may be used for research purposes only. It is not licensed for resale and may only be used by the buyer. This product may not be used and is not licensed for clinical assays, where the results of such assays are provided as a diagnostic service. If a diagnostic or therapeutic use is anticipated, then a license must be requested for the University of California. The availability of such diagnostic and therapeutic use license(s) cannot be guaranteed from the University of California.
Structure: Ly-49 family, 110 kD
Distribution: NK and NK-T cells, some CD8+ T cells
Function: NK cell activation or inhibition
Ligand/Receptor: MHC Class I Molecules
Cell Type: NK cells, NKT cells, T cells
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules
Antigen References: 1. Ryan JC, et al. 1997. Immunol. Rev. 155:79. 2. Mason LH, et al. 1997. J. Immunol. 159:4187. 3. Lanier LL. 1998. Ann. Rev. Immunol. 16:359. 4. Yokoyama W, et al. 1993. Ann. Rev. Immunol. 11:613.
Gene ID: 16634
UniProt: View information about Ly-49C/F/I/H on UniProt.org
Clone: 14B11
Regulatory Status: RUO
Other Names: Ly-49 inhibitory receptors C, F and I and the activating receptor H
Isotype: Syrian Hamster IgG