Biolegend, 108314, PE/Cyanine7 anti-mouse Ly-51 Antibody, 100μg

Ly-51 is a 140 kD protein also known as 6C3/BP-1. It is a homodimeric cell-surface glycoprotein with aminopeptidase A (APA) activity. Ly-51 is expressed on B cell progenitors, bone marrow stromal cell lines, thymic dendritic cells, and cortical epithelial cells. Ly-51 expression can be upregulated by IL-7 stimulation.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: C57BL/6 mouse Pre-B lymphoma cell line (L1-2) plus Abelson murine leukemia virus-specific cytotoxic T-cell clones
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation1,2 and immunohistochemical staining3-5, 7-8 of acetone-fixed frozen sections.
Application References(PubMed link indicates BioLegend citation): Pillemer E, et al. 1984. P. Natl. Acad. Sci. USA 81:4434. (IP) Hardy RR, et al. 1991. J. Exp. Med. 173:1213. (IP) Adkins B, et al. 1988. Immunogenetics 27:180. (IHC) Surh CD, et al. 1992. J. Exp. Med. 176:611. (IHC) Goverman J, et al. 1997. Immunol. Today 18:204. (IHC) Dumont-Lagace M, 2014. J. Immunol. 192:2219. PubMed Kvell K, et al. 2010. PLoS One 5:e10701. (FC, IHC) Griewank K, et al. 2007. Immunity 27:751. (FC, IHC)
Product Citations: Lucas B, et al. 2023. Nat Commun. 14:2066. PubMed Bai Y, et al. 2021. Front Cell Dev Biol. 9:730095. PubMed Goldfarb Y, et al. 2021. J Exp Med. 218:. PubMed Matsumura T, et al. 2022. Nat Commun. 13:7064. PubMed Zhang Y, et al. 2021. Commun Biol. 344:4. PubMed Liang Z, et al. 2017. Autophagy. 14:505. PubMed
RRID: AB_2566112 (BioLegend Cat. No. 108313) AB_2566113 (BioLegend Cat. No. 108314)
Structure: Aminopeptidase A, 140 kD
Distribution: B progenitors, bone marrow stromal lines, thymic dendritic cells, cortical epithelial cells
Function: Aminopeptidase, may function in IL-7-driven proliferation of B cell precursors
Cell Type: B cells, Dendritic cells, Epithelial cells
Biology Area: Immunology
Antigen References: 1. Goverman J, et al. 1997. Immunol. Today 18:204. 2. Morse HC, et al. 1987. J. Exp. Med. 165:920. 3. Sherwood P, et al. 1990. Int. Immunol. 2:399. 4. Lin Q, et al. 1998. J. Immunol. 160:4681.
Gene ID: 13809
UniProt: View information about Ly-51 on UniProt.org
Clone: 6C3
Regulatory Status: RUO
Other Names: 6C3/BP-1
Isotype: Rat IgG2a, κ