Biolegend, 109004, PerCP/Cyanine5.5 anti-mouse CD90.1 (Thy1.1) Antibody, 100μg

CD90, also known as Thy-1, is a 28-30 kD GPI-linked membrane glycoprotein. It is a member of the immunoglobulin superfamily and has been shown to interact with CD45 in signal transduction during lymphocyte proliferation and differentiation. CD90 is expressed on hematopoietic stem cells, neurons, thymocytes, peripheral T cells, fibroblasts, stromal cells.
100μg
Verified Reactivity: Mouse, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Mouse Thy1.1 transfected cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.06 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. * PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Clone S20007C antibody reacts with rat CD90 and mouse CD90.1 (Thy 1.1), which is expressed by AKR/J, PL, and FVB strains, but not CD90.2 strains (C57BL/6, BalB/C, etc).Clone S20007C partially blocks binding of clone OX-7 on target cells.
RRID: AB_2941383 (BioLegend Cat. No. 109003) AB_2941383 (BioLegend Cat. No. 109004)
Structure: Ig superfamily, GPI-anchored membrane glycoprotein, 28-30 kD
Distribution: Hematopoietic stem cells, thymocytes, a subset of peripheral blood T cells, early myeloid and erythroid progenitors, immature B cells, neurons, activated endothelium, mast cells, dendritic cells, glomeruli
Function: Adhesion, signal transduction, lymphocyte co-stimulation, proliferation and differentiation of hematopoietic stem cells
Ligand/Receptor: Interacts with CD45
Cell Type: Antigen-presenting cells, B cells, Dendritic cells, Endothelial cells, Fibroblasts, Hematopoietic stem and progenitors, Mesenchymal Stem Cells, Neurons, T cells, Thymocytes
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Adhesion, Cell Death, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: Campbell DG, et al. 1981. Biochem J. 195:15-30. Hosseinzadeh H and Goldschneider I. 1993. J Immunol. 150:1670-9.
Gene ID: 2183824832
UniProt: View information about CD90.1 on UniProt.org
Clone: S20007C
Regulatory Status: RUO
Other Names: Thy-1, Thy-1.1, CD90, CD90.1
Isotype: Mouse IgG2b, κ
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.