Biolegend, 109817, Alexa Fluor® 647 anti-mouse CD45.2 Antibody, 25μg

CD45.2 is an alloantigen of CD45, expressed by Ly5.2 bearing mouse strains (e.g., A, AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C57BR, C57L, C58, DBA/1, DBA/2, NZB, SWR, 129). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in the mouse that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: B10.S mouse thymocytes and splenocytes
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.06 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Application Notes: The 104 antibody does not react with mouse cells expressing the CD45.1 alloantigen. Additional reported applications (for the relevant formats) include: immunoprecipitation4, in vivo and in vitro blocking of B cell responses1,2, and immunohistochemical staining of acetone-fixed frozen sections3.
Application References(PubMed link indicates BioLegend citation): Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block) Yakura H, et al. 1986. J. Immunol. 136:2729. (Block) Suzuki K, et al. 2000. Immunity 13:691. (IHC) Shen FW, et al. 1986. Immunogenetics 24:146. (IP) Baldwin TA and Hogquist KA. 2007. J. Immunol. 179:837. Pascal V, et al. 2007. J. Immunol. 179:1751. Burman AC, et al. 2007. Blood 110:1064. Kincaid EZ, et al. 2007. J. Immunol. 179:3187. Phan TG, et al. 2007. Nature Immunol. 8:992. Nakano-Yokomizo T, et al. 2011. J. Exp Med. 208:1661. PubMed Wen T, et al. 2013. PNAS. 110:6067. PubMed Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed
Product Citations: Dalmas E et al. 2017. Immunity. 47(5):928-942 . PubMed Cautivo KM, et al. 2022. Immunity. 55:254. PubMed Lee JH, et al. 2022. Cell Rep. 38:110485. PubMed Baptista AP et al. 2019. Immunity. 50(5):1188-1201 . PubMed Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed Roufaiel M, et al. 2016. Nat Immunol. 10.1038/ni.3564. PubMed Castellanos CA, et al. 2021. Sci Immunol. 6:eabh0707. PubMed Mller K, et al. 2021. EMBO Molecular Medicine. 13(4):e13390. PubMed Yang W, et al. 2020. Mucosal Immunol. 13:788. PubMed Perner C, et al. 2020. Immunity. 53(5):1063-1077.e7. PubMed Emgård J, et al. 2018. Immunity. 143:419. PubMed Wong HS, et al. 2021. Cell. . PubMed Kelsey E Sivick et al. 2018. Cell reports. 25(11):3074-3085 . PubMed Ringel AE, et al. 2020. Cell. 183(7):1848-1866.e26. PubMed Béguelin W, et al. 2020. Cancer Cell. 37(5):655-673. PubMed Ronsard L, et al. 2021. Front Immunol. 12:730471. PubMed Seydoux E, et al. 2021. Cell Reports. 35(5):109084. PubMed Ghezraoui H, et al. 2018. Nature. 560:122. PubMed Hamilton J,et al. 2017. J Immunol. 10.4049/jimmunol.1700888. PubMed Chojnacki A, et al. 2019. Commun Biol. 2:181. PubMed Hodgson R, et al. 2022. Commun Biol. 5:1216. PubMed
RRID: AB_492871 (BioLegend Cat. No. 109817) AB_492870 (BioLegend Cat. No. 109818)
Structure: Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution: All hematopoietic cells except mature erythrocytes and platelets of the CD45.2 strain of mice
Function: Phosphatase, T and B cell activation
Ligand/Receptor: Galectin-1, CD2, CD3, CD4
Biology Area: Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family: CD Molecules
Antigen References: 1. Suzuki K, et al. 2000. Immunity 13:691.
Gene ID: 19264
UniProt: View information about CD45.2 on UniProt.org
Clone: 104
Regulatory Status: RUO
Other Names: Ly-5.2, LCA
Isotype: Mouse (SJL) IgG2a, κ