Biolegend, 110211, Alexa Fluor® 647 anti-mouse I-E, 25μg

The 14-4-4S antibody reacts with the I-E k MHC class II alloantigen. These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2 k bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins. The 14-4-4S antibody cross-reacts with cells from mice of the H-2 d , H-2 p , and H-2 r haplotypes and the rat MHC class II antigen RT1D. Cells from mice of the H-2 b , H-2 f , H-2 q , and H-2 s haplotypes do not express I-E antigen. The 14-4-4S antibody has been reported to block antigen presentation and induce differentiation of mouse cells bearing I-E k .
25μg
Verified Reactivity: Mouse, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: C3H mouse skin graft and splenocytes
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported applications (for the relevant formats) include: complement-dependent cytotoxicity1, immunoprecipitation2, in vitro induction of B-cell differentiation3, in vitro blocking of antigen presentation4, and immunohistochemical staining5,6 of acetone-fixed frozen sections.
Application References(PubMed link indicates BioLegend citation): Ozato K, et al. 1980. J. Immunol. 124:533. Nei U, et al. 1994. Int. Immunol. 6:61. (IP) Harton JA, et al. 1991. Immunogenetics 34:358. Roy M, et al. 1995. Eur. J. Immunol. 25:596. Farr A, et al. 1996. Eur. J. Immunol. 26:3185. (IHC) Desmet C, et al. 2004. J. Immunol. 173:5766. (IHC) Shih FF, et al. 2006. J. Immunol. 176:3438. (FC)
Product Citations: Li J, et al. 2022. Am J Transl Res. 14:5003. PubMed
RRID: AB_493214 (BioLegend Cat. No. 110211)
Structure: MHC class II
Distribution: B cell and activated T cells, APCs of H-2b mice
Function: Antigen presentation
Ligand/Receptor: CD3/TCR, CD4
Cell Type: Antigen-presenting cells, B cells, T cells, Tregs
Biology Area: Immunology, Innate Immunity
Molecular Family: MHC Antigens
Antigen References: 1. Watts C. 1997. Ann. Rev. Immunol. 15:821. 2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.
Gene ID: 14969
UniProt: View information about I-Ek on UniProt.org
Clone: 14-4-4S
Regulatory Status: RUO
Other Names: I-Ek, MHC class II
Isotype: Mouse (C3H.SW) IgG2a, κ