Biolegend, 110307, PE anti-mouse H-2D, 50μg

The 15-5-5 antibody reacts with the H-2D k MHC class I alloantigen expressed on nucleated cells from mice of the H-2D k haplotype. H-2D k is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. The 15-5-5 antibody weakly cross-reacts with H-2K d and with cells from mice of the H-2 f haplotype. Reactivity with other haplotypes ( e.g., a,b,p,q,r,s) has not been reported.
50μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: C3H mouse splenocytes
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications (for the relevant formats) include: complement-mediated cytotoxicity1. This clone does not cross react with other haplotypes (e.g., a, b, p, q, r, s).
Application References(PubMed link indicates BioLegend citation): Ozato K, et al. 1980. J. Immunol. 124:533. Yamamoto M, et al. 1998. J. Immunol. 160:2188. Houlden BA, et al. 1991. J. Immunol. 146:425. (IP) PubMed
Product Citations: Wautier MP, et al. 2007. Blood. 894:110. PubMed Litzler LC, et al. 2023. J Exp Med. :220. PubMed Lim S, et al. 2023. Investig Clin Urol. 64:74. PubMed Xie X, et al. 2007. J Virol. 81:229. PubMed Sido J, et al. 2015. J Leukoc Biol. 98: 435-447. PubMed Fang R, et al. 2007. Infect Immun . 75:3112. PubMed
RRID: AB_313480 (BioLegend Cat. No. 110307)
Structure: MHC class I
Distribution: Nucleated cells of the H-2Dk haplotype
Function: Antigen presentation
Ligand/Receptor: CD3/TCR, CD8
Biology Area: Immunology, Innate Immunity
Molecular Family: MHC Antigens
Antigen References: 1. Watts C. 1997. Ann. Rev. Immunol. 15:821. 2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323. 3. York I, et al. 1996. Ann. Rev. Immunol. 14:369.
Gene ID: 14964
UniProt: View information about H-2Dk on UniProt.org
Clone: 15-5-5
Regulatory Status: RUO
Other Names: MHC class I
Isotype: Mouse (C3H.SW) IgG2a, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.