Biolegend, 110706, FITC anti-mouse CD45.1 Antibody, 500μg

CD45.1 is an alloantigen of CD45, expressed by Ly5.1 bearing mouse strains (e.g., RIII, SJL/J, STS/A, DA). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in mice that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell types. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.
500μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: SJL mouse thymocytes and splenocytes
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)
Application Notes: The A20 antibody does not react with leukocytes or mouse cells expressing the CD45.2 alloantigen. Additional reported applications (for relevant formats of this clone) include: immunoprecipitation3, in vitro blocking of B cell responses1,2, immunohistochemical staining of frozen sections: OCT embedded7 and acetone-fixed4-6 (direct immunofluorescence detection with fluorochrome conjugated A20 was used in (5) and (6)).
Application References(PubMed link indicates BioLegend citation): Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block) Yakura H, et al. 1986. J. Immunol. 136:2729. (Block) Shen FW, et al. 1986. Immunogenetics 24:146. (IP) Suzuki K, et al. 2000. Immunity 13:691. (IHC-F) Werner N, et al. 2002. Arterioscler. Thromb. Vasc. Biol. 22:1567. (IHC-F) Lessner SM, et al. 2002. Am. J. Pathol. 160:2145. (FC, IHC-F) Chen CC, et al. 2005. P. Natl. Acad. Sci. USA 102:11408 (IHC-F) Pascal V, et al. 2007. J. Immunol. 179:1751. (FC) Mende I, et al. 2006. Blood 107:1383. (IHC-F, FC) Phan TG, et al. 2007. Nature Immunol. 8:992. (FC) Wither DR, et al. 2009. J. Immunol. 183:5079. PubMed Pascal V, et al.2007. J. Immunol. 179:1751. PubMed Lee SW, et al. 2009. J. Immunol. 182:6753. PubMed Takada K, et al. 2009. J. Exp Med. 206:2253. PubMed Beamer CA, et al. 2007. Am. J. Respir. Cell. Mol. Biol. 37:729. (FC) PubMed Li LX, et al. 2010. J. Immunol. 184:1728. PubMed Hosoi A, et al. 2008. Cancer Res. 68:3941. (FC) PubMed Kenna TJ, et al. 2008. Blood 111:2091. PubMed Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed
Product Citations: Koyama M, et al. 2015. J Exp Med. 212: 1303 - 1321. PubMed Guo H, Cooper S, Friedman A, et al. 2017. PLoS One. 10.1371/journal.pone.0150809. PubMed Bradley CP et al. 2017. Cell host & microbe. 22(5):697-704 . PubMed Buckley MT, et al. 2023. Nat Aging. 3:121. PubMed Lawson H, et al. 2021. Stem Cell Reports. 16:2784. PubMed Vasanthakumar A, et al. 2020. Nature. 579:581. PubMed Collin R, et al. 2020. J Immunol. 205:133. PubMed Behr FM, et al. 2021. Eur J Immunol. 51:151. PubMed Lee JH, et al. 2022. Cell Rep. 38:110485. PubMed Parga-Vidal L, et al. 2022. Eur J Immunol. 52:1095. PubMed Iberg CA, et al. 2022. Cell Rep. 39:110657. PubMed Voss LF, et al. 2023. Front Immunol. 13:1021370. PubMed Oh S, et al. 2023. Nat Biotechnol. :. PubMed Ali LR, et al. 2023. J Exp Med. 220: . PubMed Kasuya T, et al. 2023. Sci Rep. 13:1653. PubMed Ernst MPT, et al. 2023. Hemasphere. 7:e824. PubMed Patton T, et al. 2023. Cell Death Dis. 14:111. PubMed Sohn J, et al. 2022. Blood Adv. 6:2557. PubMed Meacham CE, et al. 2022. Nat Cell Biol. 24:697. PubMed McNamara HA, et al. 2020. Cell Host Microbe. 572:28. PubMed Weulersse M, et al. 2020. Immunity. 53(4):824-839.e10. PubMed Viny AD, et al. 2019. Cell Stem Cell. 25:682. PubMed Domingues AF, et al. 2020. Elife. 9:e51754. PubMed Baryawno N et al. 2019. Cell. 177(7):1915-1932 . PubMed Degn SE et al. 2017. Cell. 170(5):913-926 . PubMed Schönberger K, et al. 2022. Cell Stem Cell. 29:131. PubMed Sharma GP, et al. 2021. PLoS One. 16:e0259042. PubMed Stewart I et al. 2018. Immunity. 49(3):477-489 . PubMed Duan Q, et al. 2021. Front Cell Dev Biol. 9:761193. PubMed Chen S, et al. 2022. Cancer Discov. :. PubMed Hess NJ, et al. 2020. J Immunol. 205:272. PubMed Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed Koon H, et al. 2012. J Vis Exp. 68: 4208. PubMed Li Y, et al. 2020. Cell Rep. 30:1753. PubMed Yang X, et al. 2020. Blood Sci. 2:89. PubMed Bonnet C, et al. 2021. iScience. 24:103399. PubMed Liu X, et al. 2021. eLife. 0.416666666666667. PubMed Bartleson JM, et al. 2020. Nat Immunol. 1384:21. PubMed Brooks JF, et al. 2020. Cytotherapy. 22:436. PubMed Hayatsu N et al. 2017. Immunity. 47(2):268-283 . PubMed Tsuchiya N, et al. 2020. Cell Reports. 29(1):162-175.e9.. PubMed Inoue D, et al. 2021. Nat Genet. 53:707. PubMed Yang F, et al. 2020. Cancer Res. 80:3677. PubMed Mitchell JE, et al. 2021. Cell Reports. 35(2):108966. PubMed Yoshimi A, et al. 2019. Nature. 574:273. PubMed Vannini N, et al. 2019. Cell Stem Cell. 24:405. PubMed Uchimura T et al. 2018. Immunity. 49(6):1049-1061 . PubMed Paiva RS, et al. 2021. Eur J Immunol. 51:1968. PubMed Sandrock I, et al. 2015. PLoS One. 10: 0145010. PubMed Flommersfeld S, et al. 2021. Immunity. :. PubMed Bunn P, et al. 2014. J Immunol. 192:3709. PubMed He L, et al. 2019. iScience. 0.790972222. PubMed Kaplan BLF et al. 2018. Current protocols in toxicology. 75:11:00 25. PubMed Kleppe M et al. 2018. Cancer cell. 33(1):29-43 . PubMed Kinkel SA, et al. 2022. iScience. 25:104684. PubMed Fan Z, et al. 2020. EMBO Mol Med. 12:e11571. PubMed Ise W, et al. 2018. Immunity. 48:702. PubMed Lee S, et al. 2009. J Immunol. 182:6753. PubMed Sá da Bandeira D, et al. 2022. Cell Rep. 40:111114. PubMed Schepanski S, et al. 2022. Nat Commun. 13:4571. PubMed Mansell E, et al. 2020. Cell Stem Cell. . PubMed Lee P, et al. 2014. J Immunol. 192:178. PubMed Wan X, et al. 2018. Nature. 560:107. PubMed Booth CAG, et al. 2018. Cancer Cell. 33:274. PubMed Xu X, et al. 2018. Cell. 175:1336. PubMed Pinho S, et al. 2022. Nat Cell Biol. 24:290. PubMed Chen X, et al. 2021. Cell Rep. 37:109991. PubMed Trefzer A, et al. 2021. Cell Reports. 34(6):108748. PubMed Qin Q, et al. 2021. Cell Reports. 35(8):109161. PubMed Chatterjee D, et al. 2021. Cell Reports. 35(2):108996. PubMed Murakami K, et al. 2021. Cell Reports. 34(1):108579. PubMed Jameson K 2009. J Exp Med. 206:2253. PubMed Shin JJ, et al. 2020. Cell Rep. 32:108093. PubMed Frost JN, et al. 2021. Med (N Y). 2:164. PubMed Port JR, et al. 2020. J Virol. 94:. PubMed Sadik A, et al. 2020. Cell. 1252:182. PubMed Kretschmer L, et al. 2020. Nat Commun. 0.536805556. PubMed Wu B, et al. 2021. Immunity. 54(2):308-323.e6. PubMed Fallet B, et al. 2016. Immunology. 1: eaah6817. PubMed Hata T, et al. 2013. J Immunol. 191:83. PubMed Firl DJ et al. 2018. eLife. 7 pii: e33051. PubMed Zhang CR, et al. 2022. Blood Cancer Discov. 3:220. PubMed Stephens WZ, et al. 2021. Cell Rep. 37:109916. PubMed Zeis P, et al. 2020. Immunity. 53:775. PubMed Ulland T, et al. 2016. Nat Commun. 7:13180. PubMed Kobia FM, et al. 2020. PLoS Biol. 18:e3000850. PubMed Haniuda K, et al. 2020. Cell Rep. 33:108333. PubMed Celik H, et al. 2018. Cancer Cell. 34:741. PubMed Di Genua C, et al. 2019. Haematologica. 104:2215. PubMed Omatsu Y, et al. 2022. Nat Commun. 13:2654. PubMed Sebina I, et al. 2016. PLoS Pathog. 12:e1005999. PubMed Muri J, et al. 2020. Cell Reports. 29(9):2731-2744.e4.. PubMed van der Poel CE, et al. 2019. Cell Rep. 29:2745. PubMed Sema Kurtulus et al. 2018. Immunity. 50(1):181-194 . PubMed Wu G, et al. 2021. Nat Cancer. 2:1170. PubMed Xu Y, et al. 2021. iScience. 24:103445. PubMed Ikeda T, et al. 2014. PLoS One. 9:115198. PubMed Gundra UM, et al. 2017. Nat Immunol. 1.195833333. PubMed Wu B, et al. 2022. Nat Commun. 13:2155. PubMed Tanaka S, et al. 2021. Am J Transplant. 21:353. PubMed Alam IS, et al. 2020. Cancer Res. 80:4780. PubMed Heckler M, et al. 2021. Cancer Discov. Online ahead of prin. PubMed Xu G, et al. 2015. Free Radic Biol Med. 87: 15-25. PubMed Huang L, et al. 2015. PLoS Pathog. 11: 1005347. PubMed Vannini N, et al. 2016. Nat Commun. 7:13125. PubMed Alterauge D, et al. 2020. Cell Rep. 33:108232. PubMed Hall JA, et al. 2020. Cell Metabolism. 32(4):665-675.e6. PubMed Young K, et al. 2021. Cell Stem Cell. . PubMed Gay L, et al. 2013. Genes Dev. 27:98. PubMed Cabal-Hierro L, et al. 2020. Nat Commun. 11:1406. PubMed Tan DQ, et al. 2019. Cell Rep. 26:2316. PubMed Chen S, et al. 2018. Nat Commun. 9:5298. PubMed Okubo A, et al. 2021. Int J Mol Sci. 23:. PubMed Liu Y, et al. 2021. Nat Commun. 12:6831. PubMed Chang MH, et al. 2021. Cell Rep. 37:109902. PubMed
RRID: AB_313494 (BioLegend Cat. No. 110705) AB_313495 (BioLegend Cat. No. 110706)
Structure: Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution: All hematopoietic cells except mature erythrocytes and platelets of the CD45.1 strain of mice
Function: Phosphatase, T and B cell activation
Ligand/Receptor: Galectin-1, CD2, CD3, CD4
Biology Area: Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
Molecular Family: CD Molecules
Antigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85. 3. Kishihara K, et al. 1993. Cell 74:143. 4. Pulido R, et al. 1988. J. Immunol. 140:3851.
Gene ID: 19264
UniProt: View information about CD45.1 on UniProt.org
Clone: A20
Regulatory Status: RUO
Other Names: T200, Ly-5.1, LCA
Isotype: Mouse (A.SW) IgG2a, κ