Biolegend, 110707, PE anti-mouse CD45.1 Antibody, 50μg

CD45.1 is an alloantigen of CD45, expressed by Ly5.1 bearing mouse strains (e.g., RIII, SJL/J, STS/A, DA). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in mice that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell types. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.
50μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: SJL mouse thymocytes and splenocytes
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The CD45.1 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: The A20 antibody does not react with leukocytes or mouse cells expressing the CD45.2 alloantigen. Additional reported applications (for relevant formats of this clone) include: immunoprecipitation3, in vitro blocking of B cell responses1,2, immunohistochemical staining of frozen sections: OCT embedded7 and acetone-fixed4-6 (direct immunofluorescence detection with fluorochrome conjugated A20 was used in (5) and (6)).
Application References(PubMed link indicates BioLegend citation): Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block) Yakura H, et al. 1986. J. Immunol. 136:2729. (Block) Shen FW, et al. 1986. Immunogenetics 24:146. (IP) Suzuki K, et al. 2000. Immunity 13:691. (IHC-F) Werner N, et al. 2002. Arterioscler. Thromb. Vasc. Biol. 22:1567. (IHC-F) Lessner SM, et al. 2002. Am. J. Pathol. 160:2145. (FC, IHC-F) Chen CC, et al. 2005. P. Natl. Acad. Sci. USA 102:11408 (IHC-F) Pascal V, et al. 2007. J. Immunol. 179:1751. (FC) Mende I, et al. 2006. Blood 107:1383. (IHC-F, FC) Phan TG, et al. 2007. Nature Immunol. 8:992. (FC) Wither DR, et al. 2009. J. Immunol. 183:5079. PubMed Pascal V, et al.2007. J. Immunol. 179:1751. PubMed Lee SW, et al. 2009. J. Immunol. 182:6753. PubMed Takada K, et al. 2009. J. Exp Med. 206:2253. PubMed Beamer CA, et al. 2007. Am. J. Respir. Cell. Mol. Biol. 37:729. (FC) PubMed Li LX, et al. 2010. J. Immunol. 184:1728. PubMed Hosoi A, et al. 2008. Cancer Res. 68:3941. (FC) PubMed Kenna TJ, et al. 2008. Blood 111:2091. PubMed Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed
Product Citations: Guo H, Cooper S, Friedman A, et al. 2017. PLoS One. 10.1371/journal.pone.0150809. PubMed Weiberg D, et al. 2018. PLoS One. 13:e0205247. PubMed Li X, et al. 2023. J Clin Invest. 133:. PubMed Kozlovski S, et al. 2023. Front Immunol. 13:1041552. PubMed Rundberg Nilsson A, et al. 2023. iScience. 26:106341. PubMed Houlder E, et al. 2023. Nat Commun. 14:1863. PubMed Zhang AQ, et al. 2023. Nat Biomed Eng. . PubMed Takihara Y, et al. 2022. Commun Biol. 5:776. PubMed Miranda K, et al. 2022. iScience. 25:104994. PubMed Albayrak E, et al. 2022. J Cell Biochem. 123:2009. PubMed McAlpine CS, et al. 2022. J Exp Med. 219: . PubMed Hägglöf T, et al. 2023. Cell. 186:147. PubMed Scherer S, et al. 2023. Nat Immunol. 24:501. PubMed De Giovanni M, et al. 2022. Cell. 185:815. PubMed McNamara HA, et al. 2020. Cell Host Microbe. 572:28. PubMed Hirata Y et al. 2018. Cell stem cell. 22(3):445-453 . PubMed Suresh R, et al. 2020. J Immunother Cancer. 8:. PubMed Otano I, et al. 2021. Nat Commun. 12:7296. PubMed Koh CH, et al. 2020. Cancer Immunol Res. 8:698. PubMed MacDonald K, et al. 2014. J Immunol. 192:3180. PubMed Christian LS, et al. 2021. Cell Reports. 35(6):109118. PubMed Sano T, et al. 2021. Cell Reports. 36:109608. PubMed Stewart I et al. 2018. Immunity. 49(3):477-489 . PubMed Delás MJ, et al. 2019. Cell Rep. 27:719. PubMed Bromley SK, et al. 2020. Cell Reports. 32(9):108085. PubMed Piepke M, et al. 2021. J Neuroinflammation. 18:265. PubMed Ron–Harel N, et al. 2019. Cell Rep. 28:3011. PubMed Lechner AJ et al. 2017. Cell stem cell. 21(1):120-134 . PubMed Bowers E, et al. 2018. Nat Med. 24:95. PubMed Klarquist J, et al. 2021. Cell Rep. 36:109591. PubMed Hayatsu N et al. 2017. Immunity. 47(2):268-283 . PubMed Mann M, et al. 2018. Cell Rep. 25:2992. PubMed LaFleur MW, et al. 2019. Nat Immunol. 20:1335. PubMed Fukushima T, et al. 2019. Cell Rep. 29:4144. PubMed Fumagalli V, et al. 2020. J Exp Med. :217. PubMed Perner C, et al. 2020. Immunity. 53(5):1063-1077.e7. PubMed Lo W, Allen D 2012. Nat Immunol. 13:880. PubMed Yoshimi A, et al. 2019. Nature. 574:273. PubMed Uchimura T et al. 2018. Immunity. 49(6):1049-1061 . PubMed Nakamura‐Ishizu A et al. 2018. Cell reports. 25(7):1772-1785 . PubMed Prasad M, et al. 2021. Front Immunol. 12:644483. PubMed Tang Y, et al. 2015. Exp Hematol. 43: 554-564. PubMed Hu Z, et al. 2017. Sci Rep. 7:41558. PubMed Levine LS, et al. 2021. Immunity. 54(4):829-844.e5. PubMed Uchil PD et al. 2018. Cell host & microbe. 25(1):87-100 . PubMed Hillel–Karniel C, et al. 2020. Cell Reports. 30(3):807-819.e4.. PubMed Lissner MM, et al. 2020. Elife. 9:00. PubMed Kaplan BLF et al. 2018. Current protocols in toxicology. 75:11:00 25. PubMed Kleppe M et al. 2018. Cancer cell. 33(1):29-43 . PubMed Wu L, et al. 2022. Theranostics. 12:842. PubMed Derecka M, et al. 2020. Nat Immunol. 261:21. PubMed Morales-Mantilla DE, et al. 2022. Elife. 11:. PubMed Russler-Germain EV, et al. 2021. Elife. 10:. PubMed Mansell E, et al. 2020. Cell Stem Cell. . PubMed Ringel AE, et al. 2020. Cell. 183(7):1848-1866.e26. PubMed Mishra BB, et al. 2017. Nat Microbiol. 2:17072. PubMed Malik A et al. 2018. Immunity. 49(3):515-530 . PubMed Chen X, et al. 2021. Cell Rep. 37:109991. PubMed Bei T, et al. 2021. Molecules. 26:. PubMed Deerhake ME, et al. 2021. Immunity. 54(3):484-498.e8. PubMed LaFleur MW, et al. 2019. Nat Commun. 10:1668. PubMed Li C, et al. 2016. Nat Commun. 7:12632. PubMed Leon-Rico D, et al. 2015. Lab Anim. 49:132. PubMed Uslu M, et al. 2020. J Cell Physiol. 235:9644. PubMed Ma K, et al. 2022. iScience. 25:104347. PubMed Nelson CE et al. 2019. Cell Rep. 28(12):3092-3104 . PubMed Jaako P, et al. 2016. Leukemia. 10.1038/leu.2016.159. PubMed Ulland T, et al. 2016. Nat Commun. 7:13180. PubMed Winkler ES, et al. 2020. Cell. 182(4):901-918.e18. PubMed Evrard M et al. 2018. Immunity. 48(2):364-379 . PubMed Alexander Mildner et al. 2017. Immunity. 46(5):849-862 . PubMed Agrawal M, et al. 2022. Blood. 140:1094. PubMed Li J, et al. 2011. Dev Comp Immunol. 35:872. PubMed Pfau S, et al. 2016. Genes Dev. 30: 1395 - 1408. PubMed Danzer H, et al. 2020. Elife. 9:00. PubMed Field CS, et al. 2020. Cell Metab. 31:422. PubMed Kohn M, et al. 2021. Front Immunol. 626627:12. PubMed Kwok I, et al. 2020. Immunity. 53(2):303-318.e5. PubMed Halvarsson C, et al. 2019. Antioxid Redox Signal. 31:211. PubMed Luo H, et al. 2019. Cell Rep. 26:945. PubMed Lu Y, et al. 2018. Cancer Cell. 33:1048. PubMed Ding Z, et al. 2017. Sci Rep. 10.1038/s41598-017-12488-z. PubMed Waldman MM, et al. 2022. Front Immunol. 13:856977. PubMed Tan DQ, et al. 2019. Cell Rep. 26:2316. PubMed Hodgson R, et al. 2022. Commun Biol. 5:1216. PubMed Patterson DG, et al. 2021. J Immunol. 207:1798. PubMed Aggarwal N, et al. 2021. Cell Rep. 37:110170. PubMed
RRID: AB_313496 (BioLegend Cat. No. 110707) AB_313497 (BioLegend Cat. No. 110708)
Structure: Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution: All hematopoietic cells except mature erythrocytes and platelets of the CD45.1 strain of mice
Function: Phosphatase, T and B cell activation
Ligand/Receptor: Galectin-1, CD2, CD3, CD4
Biology Area: Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
Molecular Family: CD Molecules
Antigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85. 3. Kishihara K, et al. 1993. Cell 74:143. 4. Pulido R, et al. 1988. J. Immunol. 140:3851.
Gene ID: 19264
UniProt: View information about CD45.1 on UniProt.org
Clone: A20
Regulatory Status: RUO
Other Names: T200, Ly-5.1, LCA
Isotype: Mouse (A.SW) IgG2a, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.