Biolegend, 112709, Ultra-LEAF™ Purified anti-mouse CD210 (IL-10 R) Antibody, 100μg

CD210 is a 90-110 kD IL-10 receptor. It is a class II cytokine receptor expressed on thymocytes, T cells, B cells, NK cells, monocytes and macrophages. Ligand binding of CD210 induces Jak1 and Tyk, resulting in STAT1 and STAT3 activation. IL-10 receptor stimulation results in the inhibition of cytokine production and the costimulation of B cell proliferation and differentiation. The only known ligand for this receptor is IL-10.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Soluble mIL-10R-FLAG-His
Formulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level: Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation: The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration: The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application: FC - Quality tested Block - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for the relevant formats) include: in vitro and in vivo blocking of ligand binding1-5. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 112706) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 112704) or biotinylated anti-rat IgG (Cat. No. 405402) second step, followed by SAv-PE (Cat. No. 405204)). The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 112710) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).
Application References(PubMed link indicates BioLegend citation): O'Farrell A, et al. 1998. EMBO J. 17:1006. (Block) Wang W, et al. 2004. FASEB J. 18:1043. (Block) Vieira PL, et al. 2004. J. Immunol. 172:5986. (Block) Iwashiro M, et al. 2001. P. Natl. Acad. Sci. USA 98:9226. (Block) Vicari AP, et al. 2002. J. Exp. Med. 196:541. (Block)
Product Citations: Jian JM, et al. 2022. Neurosci Bull. 1025:38. PubMed Gottschalk RA, et al. 2019. Elife. 8:e46836. PubMed Kuan Y, et al. 2013. PLoS One. 8:72422. PubMed Hartmann W, et al. 2020. Cell Reports. 29(8):2243-2256.e4.. PubMed Murdock B, et al. 2014. J Immunol. 193:4107. PubMed
RRID: AB_11147950 (BioLegend Cat. No. 112709) AB_11149684 (BioLegend Cat. No. 112710) AB_2616679 (BioLegend Cat. No. 112711) AB_2616680 (BioLegend Cat. No. 112712) AB_2832377 (BioLegend Cat. No. 112713) AB_2832378 (BioLegend Cat. No. 112714)
Structure: Class II cytokine receptor family, 90-110 kD
Distribution: Thymocytes, T and B cells, NK cells, monocytes, macrophages
Function: Inhibits cytokine production, costimulates B cell proliferation and differentiation
Ligand/Receptor: IL-10
Cell Type: B cells, Macrophages, Monocytes, NK cells, T cells, Thymocytes
Biology Area: Immunology
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors
Antigen References: 1. Ho A, et al. 1993. P. Natl. Acad. Sci. USA 90:11267. 2. Tan JC, et al. 1993. J. Biol. Chem. 268:21053. 3. Niemand C, et al. 2003. J. Immunol. 170:3263. 4. Corinti S, et al. 2001. J. Immunol. 166:4312.
Gene ID: 16154
UniProt: View information about CD210 on UniProt.org
Clone: 1B1.3a
Regulatory Status: RUO
Other Names: IL-10R, Interleukin-10 Receptor
Isotype: Rat IgG1, κ
Q: Do you guarantee that your antibodies are totally pathogen free?
A: BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
Q: Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
A: No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
Q: Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
A: No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.
Q: Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
A: We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.