Biolegend, 112804, Biotin anti-mouse CD119 (IFN-γ R α chain) Antibody, 500μg

CDw119 is a 90 kD immunoglobulin superfamily member, also known as IFN-γRα chain. It is a class II cytokine receptor family member that serves as a IFN-γ-binding chain associated with the IFN-γ β chain also known as AF-1. In addition to ligand binding, CDw119 participates in ligand trafficking. CDw119 is expressed on T and B cells, NK cells, fibroblasts, endothelial, and epithelial cells. Binding of IFN-γ induces receptor dimerization, internalization, Jak1 and Jak2 protein kinase activation and, ultimately, STAT1 activation. IFN-γ initiates and regulates a variety of immune responses.
500μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Armenian Hamster
Immunogen: Soluble recombinant mouse IFN-γR α chain protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for the relevant formats) include: Western blotting and immunoprecipitation1. The 2E2 antibody does not block the binding of IFN-γ to IFN-γ receptor. For the most successful immunofluorescent staining results, it may be necessary to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 112804) or biotinylated anti-Armenian hamster IgG (Cat. No. 405501) second step, followed by SAv-PE (Cat. No. 405204)).
Application References(PubMed link indicates BioLegend citation): Bach EA, et al. 1995. Science 270:1215.
Product Citations: Tang Q, et al. 2022. Mol Ther. 30:2709. PubMed Kiritsy MC, et al. 2021. Elife. 10:. PubMed Bruin A, et al. 2010. Blood. 116:2559. PubMed Cella M, et al. 2010. Proc Natl Acad Sci U S A. 107:10961. PubMed Ji L et al. 2019. Immunity. 51(2):272-284 . PubMed DanDan Huang, Lang Bao 2016. J Microbiol Immunol Infect. 49:859-865. PubMed Ribechini E, et al. 2017. Blood Adv. 0.699305556. PubMed
RRID: AB_2123476 (BioLegend Cat. No. 112803) AB_2123475 (BioLegend Cat. No. 112804)
Structure: Ig superfamily, class II cytokine receptor family, associates with IFN-γ AF-1, 90 kD
Distribution: T cells and B cells, NK cells, myeloid cells, fibroblasts, endothelial and epithelial cells
Function: Initiates and regulates immune response
Ligand/Receptor: IFN-γ
Cell Type: B cells, Endothelial cells, Epithelial cells, Fibroblasts, NK cells, T cells
Biology Area: Immunology
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors
Antigen References: 1. Basu M, et al. 1988. P. Natl. Acad. Sci. USA 85:6282. 2. Codias EK, et al. 1990. J. Immunol. 144:2197.
Gene ID: 15979
UniProt: View information about CD119 on UniProt.org
Clone: 2E2
Regulatory Status: RUO
Other Names: IFN-γRα , IFN-γ Receptor α chain, CDw119
Isotype: Armenian Hamster IgG
Q: How many biotin molecules are per antibody structure?
A: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.